实施地理标志农产品品牌战略的思考

地理标志农产品品牌战略是指农产品地理标志申报成功后,对地理标志农产品品牌的推广、维护和提升等一系列有关品牌建设的长期规划.实施品牌战略,是提高地理标志农产品市场竞争力最有效的途径,它对发展中国现代农业具有重要的战略意义和现实意义.本文分析了中国地理标志农产品品牌战略的实施现状和存在问题,并提出了相关政策建议.     

Identification of Co-dominant SSR Markers Associated with Genes Controlling α′-and α-subunit-null β-conglycinin Phenotypes in Soybean (Glycine max (L.) Merr.)

Studies have shown that the three subunits of β-conglycinin are the main potential allergens of soybean sensitive patients.And β-conglycinin has adverse effects on nutrition and food processing.So solation and production of lines with lower β-conglycinin content has been the focus of recent soybean breeding projects.Soybean lines with deficiency in one or all subunits of β-conglycinin have been obtained.An effective and rapid system to identify such mutations will facilitate genetic manipulation of the β-conglycinin subunit composition.Here,two segregating F_2 populations were developed from crosses between Cgy-1/cgy-1 (CC),an α′-lacking line (Δα′),and DongNong 47 (DN47),a wild-type (Wt) Chinese soybean cultivar with normal globulin components,and Cgy-2/cgy-2 (CB),an α-lacking line (Δα),and DN47.These populations were used to estimate linkage among the cgy-1 (conferring α′-null) and cgy-2 (α-null) loci and simple sequence repeat (SSR) markers.Seven SSR markers (Sat_038,Satt243,Sat_307,Sat_109,Sat_231,Sat_108 and Sat_190) were determined to co-segregate with cgy-1,and six SSR markers (Satt650,Satt671,Sat_418,Sat_170,Satt292 and Sat_324) co-segregated with cgy-2.Linkage maps being composed of seven SSR markers and cgy-1 locus,and six SSR markers and the cgy-2 locus were then constructed.It assigned that the cgy-1 gene to chromosome 10 at a position between Sat_307 and Sat_231,and the cgy-2 gene to chromosome 20 at a position between Satt650 and Satt671.These markers should enable map-based cloning of the cgy-1 and cgy-2 genes.For different subunit-deficiency types[α′-null,α-null and (α′+α)-null types],the two sets of SSR markers could also detect of polymorphism between three normal cultivars and seven related mutant lines.The identification of these markers is great significance to the molecular marker-assisted breeding of soybean β-conglycinin subunits.     

日粮添加免疫生长促进剂C96对雏鸡若干血液指标的影响

选择１２０只１日龄ＡＡ商品健康雏鸡,随机分为４组,每组３０只。初步观察了日粮添加免疫生长促进剂Ｃ９６对血液中红细胞总数（ＲＢＣ）、比容（ＰＣＶ）、血红蛋白含量（Ｈｂ）、网织红细胞比例（ＲＣＣ）、白细胞总数（ＷＢＣ）等的影响。结果表明：肉雏鸡在１５ｄ时,日粮添加Ｃ９６为１１ｍｇ／ｋｇ,饲料的Ｃ９６组和疫苗－Ｃ９６组,与对照组和疫苗组比较,ＲＢＣ和Ｈｂ增高,ＷＢＣ显著下降,ＲＣＣ降低,ＰＣＶ无明显规律性变化;３０ｄ和４５ｄ时,添加Ｃ９６组和疫苗－Ｃ９６组的ＲＢＣ、Ｈｂ和ＰＣＶ,与对照组和疫苗组基本相近,ＷＢＣ和ＲＣＣ均比对照组和疫苗组低或明显低。但３０ｄ时疫苗组的ＷＢＣ比对照组高。整个试验期,ＲＢＣ、ＷＢＣ和ＰＣＶ随日龄的增长呈上升趋势,ＲＣＣ呈波动性下降。     

New technique of sex preselection for increasing female ratio in boar sperm model

In this study, we tried to optimize the porcine semen extender conditions to maximize the differences between live X chromosome-bearing (X) spermatozoa and to Y chromosome-bearing (Y) spermatozoa without a decline in the fertility rate at different pH conditions during storage. We observed the viability of X and Y boar spermatozoa in acidic (pH 6.2), original (pH 7.2), and alkaline condition (pH 8.2) for 5 days to investigate the effect of storage conditions on the X to Y spermatozoa ratio. The functional parameters of spermatozoa were also examined to evaluate sperm quality. Sperm motility was preserved at pH 7.2 and pH 6.2 for 3 days, while sperm motility at pH 8.2 decreased significantly after 2 days. Non-capacitated spermatozoa increased while capacitated spermatozoa decreased during storage. Sperm viability decreased significantly duration-dependent under all pH conditions, but there was no significant difference during storage at pH 6.2 and 7.2. The X: Y ratio of live spermatozoa in acidic condition was maximized (1.2:1) without affecting the sperm function and fertility-related protein expression after 2 days compared to original conditions. Moreover, insemination of sows using acidic extender increased the number of female pups on days 1 and 2 of preservation. These results indicate that the production of female offspring may increase when acidic BTS is used for 2 days without affecting the success rate of AI. Above all, this method is simple and economical compared to other methods.     

广东鹤山、东莞莲花山小蛾类（Ⅰ）

对广东省鹤山和东莞莲花山自然保护区的小蛾类昆虫进行调查，计有8科35属43种。其中广东新记录种14种。     

槽式堆肥中搅拌螺旋输送能力的研究

针对深槽式堆肥系统的搅拌螺旋物料输送能力展开研究.在特定的工作倾角下,调整搅拌螺旋的旋转速度和横向移动速度,计量物料的输送状态.实验表明:在不同的横向运动方向上,搅拌螺旋的输送能力不同;较高的转速和较低的横向移动速度有利于物料输送.     

谏壁发电厂泵站改造实践

为解决渣浆泵在运行时轴封水供应困难的问题，计算了谏壁发电厂松林山泵站的装置特必,天详细分析论证的基础上，确定了泵站的改造方案，进行了叶轮的优化设计。     

苏云金杆菌杀虫晶体蛋白作用机制的研究进展

自本世纪６０年代以来,由于化学农药引起的环境污染、害虫普遍产生抗药性等原因,生物防治越来越受到人们的重视。在现有的各种生物防治策略中,研究和利用微生物杀虫剂发展很快,最突出的方法是利用苏云金杆菌（Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓ,Ｂｔ）进行防...     

3种螯合剂对铅胁迫下香樟幼苗生长及铅富集的影响

为了研究在添加不同螯合剂的情况下,植物对土壤Pb(铅)胁迫的生理生长反应及对Pb的富集转运情况,揭示其变化机制,筛选出较好的螯合剂及适宜的质量摩尔浓度,选择香樟幼苗为试验对象,在Pb质量分数为1045 mg·kg^-1土壤中分别添加柠檬酸、苹果酸和乙二胺四乙酸(EDTA)3种螯合剂,每种螯合剂分别设置了0、2、4、6、8 mmol·kg^-15个质量摩尔浓度,测量香樟幼苗的生长指标、生理指标以及Pb富集转运等指标并进行统计分析。结果表明:3种螯合剂对香樟幼苗生长生理及Pb的富集转运均有显著影响,随着3种螯合剂质量摩尔浓度的增加,Pb胁迫加重;同一质量摩尔浓度下,多数指标,经EDTA处理的分别与经柠檬酸和苹果酸处理的结果有显著差异,柠檬酸和苹果酸处理二者之间差异不显著;EDTA质量摩尔浓度不大于2 mmol·kg^-1,柠檬酸、苹果酸质量摩尔浓度不大于4 mmol·kg^-1,香樟生长状况及Pb吸收综合效果最好。综合分析得出:柠檬酸、苹果酸、EDTA都具有促进植物吸收土壤Pb的作用,3种螯合剂均能提高香樟幼苗的富集系数和转移系数;当土壤Pb质量分数为1045 mg·kg^-1、EDTA质量摩尔浓度为2 mmol·kg^-1、柠檬酸和苹果酸质量摩尔浓度为4 mmol·kg^-1时,香樟幼苗吸收土壤Pb的效果最佳。在达到同等效率的前提下,建议优先选用柠檬酸或苹果酸为宜。