全文获取类型
收费全文 | 96篇 |
免费 | 12篇 |
国内免费 | 1篇 |
专业分类
林业 | 4篇 |
基础科学 | 1篇 |
2篇 | |
综合类 | 11篇 |
农作物 | 2篇 |
水产渔业 | 7篇 |
畜牧兽医 | 79篇 |
植物保护 | 3篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2020年 | 4篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2017年 | 3篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 2篇 |
2013年 | 10篇 |
2012年 | 4篇 |
2011年 | 5篇 |
2010年 | 3篇 |
2009年 | 4篇 |
2007年 | 2篇 |
2006年 | 4篇 |
2005年 | 2篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2000年 | 1篇 |
1998年 | 6篇 |
1997年 | 7篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
排序方式: 共有109条查询结果,搜索用时 515 毫秒
81.
82.
83.
84.
Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil 下载免费PDF全文
MO Koch RR Weiss AA Cruz VT Soccol KA Gonçalves MAF Bertol OC Beltrame RL Dittrich 《Reproduction in domestic animals》2016,51(4):550-554
The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii. 相似文献
85.
M. ÍHA J. KUBEKA M. VAEK J. SEA T. MRKVIKA M. PRCHALOVÁ J. MATNA M. HLADÍK M. ECH V. DRATÍK J. FROUZOVÁ E. HOHAUSOVÁ O. JAROLÍM T. JZA M. KRATOCHVÍL J. PETERKA & M. TUER 《Fisheries Management and Ecology》2009,16(2):121-129
Abstract The inshore fish community of the Římov Reservoir in the Czech Republic was evaluated over 21 years using shore seining at night. The development of the fish community was divided into two separate phases: a highly dynamic and unstable phase dominated by perch, Perca fluviatilis L., was replaced by an extremely stable cyprinid phase dominated by roach, Rutilus rutilus (L.), and bream, Abramis brama (L.). The abundance of both these species oscillated during the cyprinid phase, but with decreasing amplitude. The proportion of piscivorous fish species such as asp, Aspius aspius (L.), pike, Esox lucius L., and pikeperch, Sander lucioperca (L.), increased slightly with time but remained low. The biomass of large Cladocera was negatively correlated with fish biomass only during the perch phase. The Shannon–Weaver index of diversity increased during the fish community succession, mainly because of greater evenness amongst the species. 相似文献
86.
RÉKA DOMOKOS 《Fisheries Oceanography》2009,18(6):419-438
The South Equatorial Counter Current (SECC) strongly influences the American Samoa Exclusive Economic Zone (EEZ) and changes strength on a seasonal and ENSO cycle. A strong SECC is associated with a predominantly anticyclonic eddy field as well as increased micronekton biomass and catch-per-unit-effort (CPUE) for albacore tuna, the economically important target species of the local longline fishery. A strong SECC carries chlorophyll a -rich waters from upwelling regions at the north coast of New Guinea towards the EEZ, most likely resulting in the observed increase in micronekton biomass, forage for albacore. Relatively stable anticyclonic eddies show a further increase in micronekton biomass, apparently advected from neighboring SECC waters. The presence of forage presumably concentrates albacore, thus resulting in the observed increase in CPUE. High shear regions of neither anticyclonic nor cyclonic eddies correlate with increased micronekton biomass. Areas characterized by South Equatorial Current (SEC) waters correspond to areas with the lowest micronekton biomass and the highest number of aggregative structures, which are most likely small pelagic fish shoals. Micronekton composition in SEC waters differs from that in the SECC. During El Niños, the seasonal signals at the north shore of New Guinea and in the SECC are exceptionally strong and correspond to higher albacore CPUE in the EEZ. My results suggest that the strength of upwelling and the resulting increase in chlorophyll a at New Guinea, as well as the Southern Oscillation Index, could be used to predict the performance of the local longline fishery for albacore tuna in the American Samoa EEZ. 相似文献
87.
ABSTRACTAims: To determine the presence of infection and co-infection of Plasmodium lineages in introduced birds at translocation sites for the North Island saddleback (Philesturnus rufusater), to investigate their role as Plasmodium spp. reservoirs.Methods: Blood samples were collected from introduced bird species, with a special focus on blackbirds (Turdus merula) and song thrushes (Turdus philomelos), at six locations in the North Island of New Zealand that were the origin, or translocation sites, for North Island saddleback. Where available, blood smears were examined, and blood samples were tested using nested PCR with subsequent sequence analysis, for the presence of Plasmodium spp.Results: Of the 55 samples tested using PCR analysis, 39 (71%) were positive for Plasmodium spp., and 28/40 (62%) blood smears were positive for Plasmodium spp. Overall, 31 blood samples were from blackbirds with 28/31 (90%) samples positive for Plasmodium spp. Six distinct avian Plasmodium lineages were identified, including three cosmopolitan lineages; Plasmodium vaughani SYAT05 was detected in 16 samples, Plasmodium matutinum Linn1 in 10 samples and Plasmodium elongatum GRW6 in eight samples. Mixed infections with more than one lineage were detected in 12 samples. Samples from two Australian magpies (Gymnorhina tibicen) were positive for Plasmodium. sp. lineage MYNA02, previously not identified in New Zealand.Conclusions and clinical relevance: This is the first report from New Zealand in which specific Plasmodium spp. mixed infections have been found in introduced birds. Co-infections with several cosmopolitan Plasmodium lineages were identified, as well as the first report in New Zealand of an exotic avian Plasmodium sp. lineage, in Australian magpies. Whilst the role of introduced birds in maintaining and spreading pathogenic avian malaria in New Zealand is unclear, there is a potential infection risk to native birds, especially where distributions overlap. 相似文献
88.
美国黑核桃组织培养技术 总被引:19,自引:12,他引:7
采用组织培养方法对美国黑核桃进行组织培养研究。结果表明:获得外植体的最佳方法是用新鲜且经层积处理的种子育苗.再用其实生苗的顶芽或茎段;适合黑核桃茎尖(段)培养的培养基为1/2MS(、其中大量元素用量减半,微量元素用量不变).且培养基中其他物质的含量为.VB15mg/L.VB62mg/L.烟酸2mg/L.腺素2mg/L.肌醇按1/2MS量.6-BA1.5mg/L.NAA0.05mg/L;外植体接种用流水冲洗.适当缩短转瓶周期(每10~l5d转瓶一次).可减轻褐变现象。 相似文献
89.
90.
Bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction 总被引:2,自引:0,他引:2
SUMMARY Eight sheep vaccinated with 106 pfu of attenuated Australian bluetongue virus serotype 23 (BTV-free sheep were challenged with virulent BTV 23. There was little subsequent variation in the mean clinical score, or in the mean lymphocyte and platelet concentrations in the peripheral blood of the eight vaccinated sheep. There was a marked thrombocytopenia and lymphopenia in the naive sheep as the mean lymphocyte and platelet concentrations fell to a minimum at days 8 and 11 after inoculation, respectively. Similar changes were observed in three other naive sheep inoculated with field isolates of BTV 1, 9 or 23. BTV was detected by nested polymerase chain reaction in whole blood of these sheep between days 6 and 28, in mononuclear leukocytes between days 3 and 14, and in platelets between days 6 and 21. 相似文献