Properties of the immunosuppressive factor induced by murine cytomegalovirus

Murine cytomegalovirus infection of spleen cultures induced the production of a small (less than 10,000 molecular weight) immunosuppressive factor (VISF), which suppressed concanavalin-A mitogenesis in fresh mouse spleen cells, and in fresh human peripheral blood leukocytes. The factor did not affect the growth of two murine T-cell lines or of mouse fibroblasts. A similar factor was also found in the serum of infected mice, at the time of maximum immune suppression. The properties of VISF indicate that the mechanism of MCMV immune suppression is different from that caused by several other viruses which are important in human and veterinary medicine.     

Effects of activation methods on DNA synthesis and development of parthenogenetic porcine embryos

This study investigated the timing of DNA synthesis and patterns of pronuclear (PN) formation during the first cell cycle, and its influence on developmental competence, velocity and proliferation index of porcine parthenote blastocysts produced by different activation treatments. Oocytes were activated as follows: electrical stimulation (EST), EST combined with 7.5 μg/ml cytochalasin B (EST + CCB), 10 μg/ml cycloheximide (EST + CHX) and 1.9 mm 6-dimethylaminopurine (EST + 6-DMAP) for 3 h. DNA synthesis and PN formation were evaluated using 1 mm 5'bromo-2'deoxy-uridne (BrdU) at 2 h intervals from 1 to 13 h or 5 to 13 h of post-activation (hpa), respectively. In EST, DNA synthesis started at 3 hpa, reached the peak at 11 hpa and decreased at 13 hpa. Treatment with 6-DMAP resulted in an early increase of DNA synthesis at 3 hpa, whereas CCB delayed DNA synthesis for 2 h. In EST and EST + 6-DMAP, most of the eggs showed 1PN, whereas, incidence of 2PN in EST + CCB was higher than 1PN. EST + CHX was observed with 1PN, 2PN and multiple PN. Blastocyst rate in EST + CCB and EST + 6-DMAP were significantly (p<0.05) higher than EST + CHX. But, the developmental velocity was not different among groups. Proliferation index of blastocysts, as indicated the number of blastomere at S-phase of the cell cycle was low in all groups. In conclusion, CCB, CHX and 6-DMAP used for producing porcine parthenogenetic embryos induced different onset of DNA synthesis and PN, but they did not affect the subsequent embryo development.     

High diversity,expanding populations and purifying selection in phytoplasmas causing coconut lethal yellowing in Mozambique

In this study, the putative phytoplasma species causing coconut lethal yellowing disease in Mozambique and Tanzania were characterized. The 16S rRNA and secA genes were sequenced. Phylogenetic analysis revealed that Mozambican coconut phytoplasmas belong to three different types: ‘Candidatus Phytoplasma palmicola’ 16SrXXII‐A, a second strain that was previously isolated in Tanzania and Kenya (16SrIV‐C), and a third strain that was different from all known lethal yellowing phytoplasma species. The third strain potentially represents a novel species and is closely related to pine phytoplasma. Co‐infection with ‘Ca. Phytoplasma pini’‐related and ‘Ca. Phytoplasma palmicola’ 16SrXXII‐A strains was observed. Furthermore, sequence variation in ‘Ca. Phytoplasma palmicola’ at the population level was consistent with purifying selection and population expansion.     

Evidence for divergence of restriction fragment length polymorphism patterns following in vivo replication of porcine reproductive and respiratory syndrome virus

OBJECTIVE: To determine stability of the restriction fragment length polymorphism (RFLP) pattern of a porcine reproductive and respiratory syndrome vaccine virus and patterns of other viral strains as they replicate in pigs. SAMPLE POPULATION: Field samples of porcine reproductive and respiratory syndrome virus (PRRSV) and samples from 2 weaned pigs, 2 nursery-age pigs, and 5 gilts experimentally infected with PRRSV. PROCEDURE: PRRSV was isolated from field samples, experimentally infected pigs, or pigs that were in contact with experimentally infected pigs. For each virus, RNA was isolated from infected cells, and RFLP patterns were determined. RESULTS: 61% of field samples had 2-5-2 RFLP patterns characteristic of the vaccine virus, 32% had field virus RFLP patterns, and 7% had intermediate RFLP patterns that indicated a virus with a close relationship to the vaccine virus. Viruses isolated from experimentally infected pigs had no change in RFLP patterns after up to 13 weeks of in vivo replication and transmission to contact pigs. CONCLUSIONS AND CLINICAL RELEVANCE: RFLP patterns distinguish the vaccine and field strains of PRRSV; however, as the vaccine virus spreads among a swine population, the RFLP pattern can change to a related intermediate pattern. A glycine at residue 151 of open reading frame 5 is another marker for the vaccine virus; this glycine is rapidly lost and eventually replaced with arginine as the vaccine virus replicates in pigs.     

Morphological quality of sweet corn (Zea mays L.) ears as response to soil moisture tension and phosphate fertilization in Campeche, Mexico

In Mexico, corn production, part of which is sweet corn, is mainly destined for human consumption. In the present work, the morphological quality of sweet corn ears was assessed in response to four levels of soil moisture tension indicating irrigation start (−5, −30, −55, and −80 kPa) and three levels of phosphate fertilization (60, 80 and 100 kg ha⁻¹) in carstic soils in the south-east of Mexico. A factorial experimental design with three replicates was used. The following variables were determined: fresh weight (SCFWh), dry weight (SCDWh), diameter (SCDh), and length (SCLh) of sweet corn ears, all without husk, as well as number of kernels (NKxE), number of unfilled kernels (NUK), number of rows (NRxE), and dry kernel weight per ear (DKW). Yield of fresh (YFSCh) and dry (YDSCh) sweet corn ears, both without husk, and the harvest index (HI) were also determined. HI did not show significant statistical differences among irrigation or fertilization treatments. Regarding the other variables, the effect of the more humid treatments (−5 and −30 kPa) and the effect of the higher phosphorus doses (80 and 100 kg ha⁻¹) were statistically equal (P ≤ 0.01) with the lowest NUK and the highest values of all other variables; therefore, irrigation start at soil moisture tension of −30 kPa and phosphate fertilization application of 80 kg ha⁻¹ are recommended. At this level of soil moisture, the mean values over the three fertilization levels and all the replicates, obtained for SCFWh, SCDh, SCLh and NKxE were 198.5 g, 4.39 cm, 26.72 cm and 467 grains, respectively. According to the regression models, moisture tensions from −11.8 to −24.0 kPa, and phosphate fertilization doses from 87.7 to 102.2 kg ha⁻¹ minimize NUK and maximize the values of the rest of the variables. The highest irrigation water use efficiency was found in the moisture tension treatment of −30 kPa with an increase of 27 kg ha⁻¹ ears for each millimeter of applied irrigation water.     

THE INFLUENCE OF SUPPLEMENTARY FEEDING ON THE HERBAGE INTAKE AND GRAZING BEHAVIOUR OF DRY COWS

The herbage intake and grazing behaviour of 4 dry cows on autumn grass receiving 2, 4, 6 or 8 kg concentrates/day, was assessed in a 4×4 Latin Square experiment. Total OM intake increased from 11±5 kg/day with 2 kg concentrates to 13±6 kg/day with 8 kg concentrates. The average supplementary effect was 0±46 kg increase in OM intake/kg OM fed as concentrates. Minutes per day spent grazing decreased from 495 with 2 kg concentrates to 359 with 8 kg. The mean decline was 22 min./kg concentrates or 28 min./kg OM in concentrates eaten. Herbage was eaten at an average rate of 47 min./kg OM.     

Objective method for evaluation of texture of dehydrated mashed potatoes using sensory evaluation as a guideline

The texture of four reconstituted dehydrated mashed potato products was evaluated subjectively. Freshly mashed potatoes served as a reference. Among textural characteristics such as firmness, smoothness and glueyness it was found that glueyness correlated most strongly with the overall textural quality of the samples. A texturometer consisting of a load cell, a vertically driven plunger and a recorder was used to evaluate objectively firmness and glueyness of the samples by measuring the forces needed to compress a sample and to pull the plunger from the compressed sample. Bulk density of the samples was also determined. The objective glueyness correlated strongly with both subjective glueyness and overall textural quality scores. Density correlated quite strongly with objective firmness, objective glueyness and overall textural quality scores. The method and equipment used to measure glueyness appears to have promise as a quality control and/or product development tool in the dehydrated mashed potato industry.     

Harvesting potatoes with minimum damage

Temperature, tuber condition and harvester operation must be considered if potatoes are to be harvested with minimum damage. In general, total damage increased as temperature decreased; however, the type of damage resulting from impact was influenced by temperature and tuber condition. The data indicate that a tuber turgidity level occurs wherein total damage susceptibility level is minimal for a given tuber temperature and that the proper tuber turgidity level changes as temperature changes. A tuber hydration level which produces little damage when bruised at a flesh temperature of 65–70 F results in severe damage (shatter bruise) when subjected to the same force at 45–50 F. Conversely, a hydration level which results in severe blackspot at 65–70 F may result in slight to moderate total damage when subjected to the same impact at 50–55 F. Data from several commercial harvester studies indicate that damage may increase rather than decrease with a reduction of harvester speed. The ratio of chain speeds to forward speed was found to be more important than forward speed alone. The optimum ratios varied and were dependent on potato yield. Theoretical ratios based on the volume of tubers at different yield levels and harvester chain capacities were calculated and compared with actual ratios of commercial harvest operation. Harvesters operated with chain speed to forward speed ratios near the theoretical ratios resulted in low tuber damage (25%) with forward speed ranges of 1.60 to 3.25 mph. Harvesters operating with ratios differing considerably from theoretical ratios resulted in a high level of damage (66%). The catechol test identified shatter bruise damage which ruptured the skin, but did not detect internal blackspot. Therefore, the catechol test should be used with discretion when attempting to determine the total damage level. Lye peeling and abrasive peeling identified both blackspot and severe shatter bruise damage and should be used when determining harvester operation speeds when both types of damage occur.