麦田抗性生物型猪殃殃对苯磺隆的抗性机制

为探讨猪殃殃Galium aparine抗药性生物型(R)对苯磺隆的抗性机制,测定了苯磺隆对猪殃殃抗性、敏感(S)生物型体内靶标酶 、代谢酶 及抗氧化酶 影响的差异。离体试验结果表明,苯磺隆对R、S生物型猪殃殃ALS的抑制中量(IC50)分别为0.682、0.718 μg/L(有效剂量),R、S生物型猪殃殃ALS对苯磺隆的敏感性不存在差异。活体试验结果表明,苯磺隆茎叶喷雾处理后,R、S生物型ALS活力均表现为先上升,但S生物型上升幅度小,且随后快速下降,第3 d即回落至对照之下,并维持在低于对照的水平,而R生物型ALS活力在第2 d可达对照的4.10倍,第5 d 基本回落至对照水平,之后基本维持在对照水平;R生物型GSTs活力在第1 d即开始上升,最高可达对照的 2.40 倍,而S生物型则表现为先下降,然后小幅回升,最高为对照的1.61倍,两者在10 d左右均回落至对照水平;R生物型SOD活力与对照基本相同,而S生物型虽略有下降,但R、S间不存在显著差异;两者POD活力虽均有大幅提高,但亦不存在显著差异。结果表明,低水平抗药性生物型猪殃殃对苯磺隆产生抗性的原因可能是ALS过量表达及GSTs对苯磺隆的代谢作用加强,而不是由于ALS的敏感性下降,同时POD、SOD在减轻药害中也具有一定作用。     

露地越冬春甘蓝新品种‘皖甘 8 号’

 ‘皖甘8号’甘蓝是以自交不亲合系9802-6与9701-3杂交育成的露地越冬春甘蓝一代杂种,具有抗寒性和耐抽薹性好,抗黑腐病等特点;叶球紧实,牛心形,单球质量1.2 kg;越冬栽培生育期145 d;适合我国黄河以南地区,尤其长江流域作越冬春甘蓝种植。     

野生药用花卉资源在北京小龙门森林公园的应用分析

在景观趋同的时代,野生花卉作为一种集自然美、抗性强、极具地方特色等优点于一身的种质资源,是体现地方景观风格特征的重要方面。通过对小龙门森林公园野生植物资源的调查和整理,该研究总结了具有较高观赏价值的药用野生花卉42种,隶属于20科,36属。并且对其应用于药草园的可行性进行了分析,旨在为打造有北京特色的药草园提供一定的参考。     

三种基因型羽衣甘蓝筛选适合叶绿体转化材料

以3种不同基因型的羽衣甘蓝品种"皱叶红心"、"白鸥"和"红鸥"为试材,以MS培养基为基础培养基,分别添加不同质量浓度的6-BA、NAA、IBA激素,筛选不同品种真叶叶片不定芽的诱导频率,同时确定不同材料对除草剂双丙氨膦和抗生素壮观霉素的致死临界质量浓度,为羽衣甘蓝的叶绿体转化研究筛选受体材料。结果表明:影响羽衣甘蓝叶片诱导不定芽因素中,激素质量浓度的影响比品种的影响更大;当诱导培养基中的6-BA质量浓度为1.0mg/L、NAA质量浓度为0.1mg/L时,诱导效果最佳,是3个品种的最适培养基;按照由高到低的顺序排列,3个品种的不定芽诱导率依次为"红鸥"、"白鸥"和"皱叶红心",因此择优利用品种"红鸥"作为后续转基因研究的受体材料;分别利用双丙氨膦和壮观霉素的不同质量浓度梯度进行筛选,研究二者对"红鸥"品种叶片分化的影响,发现双丙氨膦和壮观霉素的最低抑制质量浓度分别为4mg/L和50mg/L。该研究结果对今后利用叶绿体遗传转化技术改良羽衣甘蓝品种提供了技术支持。     

负载量对宁夏设施草莓光合作用和果实品质的影响

研究不同果实负载量处理对宁夏设施草莓生长发育、光合作用和果实品质的影响。结果表明:花期进行疏果,能显著增加草莓叶面积,提高果实中性转化酶、蔗糖合酶和细胞壁转化酶活性,降低了酸性转化酶活性、叶绿素含量和净光合速率,果实品质得到显著提高。每株草莓留果量超过2个果实后,产量不再显著增加。在设施环境条件下,花期疏果,可通过调节果实内蔗糖代谢关键酶活性调节库容量和库强,加快叶片发育和加强光合作用,最终提高果实的品质。     

不同架式对设施葡萄生长发育和主芽坏死的影响

为了提高设施葡萄的产量与品质,通过不同的架式处理,研究其对设施"红提"葡萄枝蔓生长发育、果穗高度、主芽发育和果实品质的影响。结果表明,F形和L形架式显著的降低1 a生枝蔓的长度和粗度,降低了平均单果穗高度,显著提高了枝蔓花芽的形成率和主芽的萌芽率,与对照相比,F形和L形架式显著提高了葡萄的产量与品质。因此在宁夏设施环境下,合理的架式可缓和红提葡萄的生长势,调节主芽的坏死率和萌芽率,最终影响葡萄的产量与品质。     

The mechanism of apoptosis induced by homoharringtonine in HL-60 cells

AIM: To study the signal transduction pathway of apoptosis initiation induced by homoharringtonine in HL-60 cells. METHODS: After establishing the model of apoptosis initiation induced by homoharringtonine in HL-60 cells, at the point of apoptosis initiation, molecular caspase-3, Bcl-2, Bax and Fas/FasL were measured with flow cytometry and transmission electron microscope. ERK2 and P38 expression in HL-60 cells were detected by using immunohistochemistry. RESULTS: The model of apoptosis initiation induced by homoharringtonine was established in HL-60 cells. At the point of apoptosis initiation, upregulation of caspase-3 and decrease in Bcl-2/Bax were observed. However, the expression of Fas/FasL did not significantly change. ERK2 expression decreased and P38 expression increased. CONCLUSIONS: Caspase-3, Bcl-2, Bax and mitogen activated protein kinase pathways were involved in signal transduction of apoptosis initiation induced by homoharringtonine in HL-60 cells.     

秋季叶面喷施尿素对酿酒葡萄光合作用和果实品质的影响

以“赤霞珠”葡萄为试材,在秋季对叶面喷施不同浓度的尿素,研究其对叶片光合作用和果实品质的影响,以期为提高酿酒葡萄叶片的光合作用和果实品质提供理论依据.结果表明:在酿酒葡萄的生长后期,叶面喷施1％～4％的尿素可明显提高葡萄叶片气孔导度和叶绿素含量,提高了叶片中蛋白质含量和光合作用关键酶活性,净光合速率也得到明显的提高.其中,3％尿素处理不但显著提高了叶片光合作用关键酶活性和净光合速率,也显著提高了酿酒葡萄果实的品质.因此,秋季叶面喷施适当浓度的尿素可通过调节气孔导度、叶绿素含量和光合作用关键酶活性来提高叶片光合速率,最终提高果实品质.     

Expression of Gab2 in human osteosarcoma U2-OS cells

AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line.