Occurrence of scab of Phaseolus vulgaris caused by Elsinoë phaseoli in South Africa

Scab of beans ( Phaseolus vulgaris ) caused by Elsinoë phaseoli Jenkins is reported for the first time from South Africa. The conidial state (a Sphaceloma sp.) was isolated in pure culture from symptomatic plants. Typical scab symptoms were produced on plants inoculated with the Sphaceloma sp. and the pathogen was re-isolated from the scab lesions. Asci and ascospores typical of E. phaseoli were found on lesions on artificially inoculated plants and on plants from the field.     

A survey of bone fractures in the dog and cat

A survey was made of 284 canine and 298 feline fractures admitted to a metropolitan small animal hospital over a 2-year period. Approximately 80% of fractures occurred in animals less than 3-years old. Males were more commonly involved than females in both species. Road accidents were the main cause of fracture but falls and crush injuries were more common in dogs than cats. Bones most commonly affected in the cat were femur (28·2%), pelvis (24·8%) and mandible (11·4%), and in the dog radius and ulna (17·3%), pelvis (15·8%), femur (14·8), and tibia (14·8%). Forty percent of fractures were treated conservatively or by external fixation, 46·8% by internal fixation, and 13-1% were not treated. Results were satisfactory in 96·7% of treated cases.     

Sodium fertilizer application to pasture. 4. Effects on mineral uptake and the sodium and potassium status of steers

Mineral availability and status in body tissues was examined with four rumen-fistulated steers arranged in a Latin-square change-over design. The steers were fed perennial ryegrass herbage from a pasture fertilized with four Na treatments: 0 (Nil), 32 (Low), 66 (Medium) or 96 (High) kg Na ha^-1 yr^-1. Na fertilizer increased herbage contents and intakes of Na, Mg and Ca but did not affect intakes of K, P and S. Herbage K:Na and K:(Ca+Mg) decreased in direct proportion to the amount of Na fertilizer applied. With increasing dietary Na intake, the Na content of saliva increased and the K content decreased. There was an increase in Na and a reciprocal decrease in K contents of rumen fluid with increasing dietary Na. Blood plasma Na contents were greater when the Na-treated herbages were fed than in the Nil treatment. There was no effect of treatment on blood plasma K content. Increasing dietary Na increased urinary Na but did not affect urinary K. Faecal mineral concentrations of Na, Ca, P and S were increased by increasing dietary Na, whereas K content was reduced and the Mg content was unaffected. There was no difference between treatments in the proportion of mineral rapidly solubilized, except Na which increased with increasing dietary Na content. The fractional rate of degradation was greater for Na, K, and Mg than for Ca and P. Based on effective solubilization, minerals were ranked in the order K > (Mg = Na) > Ca > P, and treatments were in the order Medium > (High = Low) > Nil. Increasing Na intake increased Ca but not P or Mg availability and increased the uptake of all three minerals.     

Medial collateral ligament desmitis of the distal interphalangeal joint in the hindlimb of a horse: treatment with cast immobilisation

A 12‐year‐old show‐jumping mare was presented for investigation of a chronic hindlimb lameness of 16 weeks duration. Perineural anaesthesia and ultrasonography localised the lesion to the medial collateral ligament of the distal interphalangeal joint of the left hindlimb. Treatment consisted of a heavily padded distal limb cast for 6 weeks, strict box rest and a strictly regulated hand walking program. Serial ultrasonographic examinations were performed throughout the rehabilitation period. Collateral ligament desmitis of the distal interphalangeal joint is a commonly diagnosed condition of the forelimb; however, hindlimb collateral ligament desmitis has been rarely reported. Ultrasonographic examination in this case was an invaluable diagnostic and follow‐up tool. Immobilisation of the foot by means of a heavily padded distal limb cast allowed excellent healing of the ligament and is presented as a novel treatment for collateral ligament desmitis. At the time of writing the mare had returned to her previous level of exercise and was showing no signs of lameness.     

Sodium fertilizer application to pasture. 5. Effects on herbage digestibility and mineral availability in sheep

The effects of feeding herbages fertilized with 0 (nil), 32 (low), 66 (medium) or 96 (high) kg of sodium per hectare per year to sheep on herbage digestibility and mineral availability were examined. Modified acid detergent fibre (MADF), ash, magnesium (Mg) and phosphorus (P) contents were increased by the application of sodium (Na) treatments, while potassium (K), sulphur (S) and true protein (TP) contents were reduced. Dry matter (DM) and MADF intakes were increased in the low and medium treatments and DM and MADF digestibility increased in all Na-treated herbages. The medium- and high-sodium treatments increased salivary Na content and Na/K ratio and decreased K content. Blood K content was not affected by treatment. Intake, faecal output, apparent availability, urinary output and balance of Na were increased by the application of Na treatments. Potassium intake, balance, apparent retention and urinary output were increased by the low, unaffected by the medium and reduced by the high sodium treatment. The low and medium Na treatments increased the intake and apparent availability of Mg; Mg balance and apparent retention were increased by all Na treatments. Feeding the Na-treated herbages increased intakes, apparent availability, balance and apparent retention of Ca, P and total cations.
The low and medium Na treatments increased S intake, faecal output and apparent retention. It is suggested that through increasing the balance and apparent retention of minerals, the application of sodium fertilizer to pasture will improve the mineral status of sheep, particularly at the low and medium application rates employed in this experiment.     

Reaction time as a function of the cardiac cycle in young adults

Simple reaction times to auditory stimuli varied with the phase of the cardiac cycle in which the stimuli were presented, tending to be fastest to stimuli presented during the P-phase of the electrocardiogram. One hundred reaction times obtained from each of 56 men and women between the ages of 20 and 30 years were analyzed.     

Oil-Water Interface Templating of Mesoporous Macroscale Structures

Ordered mesostructured porous silicas that are also macroscopically structured were created by control of the interface on two different length scales simultaneously. Micellar arrays controlled the nanometer-scale assembly, and at the static boundary between an aqueous phase and an organic phase, control was achieved on the micrometer to centimeter scale. Acid-prepared mesostructures of silica were made with the p6, Pm3n, and the P63/mmc structures in the form of porous fibers 50 to 1000 micrometers in length, hollow spheres with diameters of 1 to 100 micrometers, and thin sheets up to 10 centimeters in diameter and about 10 to 500 micrometers in thickness. These results might have implications for technical applications, such as slow drug-release systems or membranes, and in biomineralization, where many processes are also interface-controlled.     

The effect of PUFA enriched Artemia on growth, survival and lipid composition of western rock lobster, Panulirus cygnus, phyllosoma

Western rock lobster, Panulirus cygnus, phyllosoma were grown from hatching to stage IV. Larvae were fed with Artemia enriched with a (i) base enrichment (Base) containing 520 g kg^?1 squid oil or tailor made enrichments in which oils high in polyunsaturated fatty acid (PUFA) have been added at the expense of squid oil. These treatments were (ii) base enrichment supplemented with docosahexaenoic acid (DHA) rich oil, (iii) base enrichment supplemented with arachidonic acid (AA) rich oil, or (iv) base enrichment supplemented with DHA and AA (D + A) rich oils. Total survival of phyllosoma to stage IV was high, with no significant difference between treatments (range 12.3–17.5%). By stage IV, the larvae fed the DHA or AA enriched Artemia were significantly larger (3.33 mm length) than larvae fed the Base or D + A enriched Artemia (3.18–3.24 mm length). Phyllosoma were sampled at stages II and III for biochemical analysis. The major lipid class (LC) in all phyllosoma was polar lipid (PL) (88.9–92.4%), followed by sterol (ST) (6.2–9.7%). Triacylglycerol (TAG), free fatty acid (FFA) and hydrocarbon/wax ester were minor components (≤1%) in all phyllosoma samples. In contrast, the major LC in all enrichments and enriched Artemia was TAG (76.3–85.1% and 53.4–60.2%, respectively), followed by PL (11.4–14.8% and 30.6–38.1% respectively). The main fatty acids (FA) in phyllosoma were 16:0, 18:1n‐9, 18:1n‐7, 18:0, AA, eicosapentaenoic acid (EPA) and DHA. Addition of AA, and to a lesser extent DHA, to enrichments resulted in increased levels of those FA in Artemia and phyllosoma compared with the Base enrichment. This was particularly evident for stage III larvae. Comparatively, elevated growth for phyllosoma to stage IV was achieved with DHA and AA enriched diets. Our findings highlight the importance of lipids and in particular essential long‐chain PUFA, as nutritional components for phyllosoma diets.