天祝县草产业发展的制约因素及其对策

通过分析天祝县草产业发展现状，指出天祝县草产业发展存在的问题，提出要大力发展天祝县草产业必须加强天然草原综合改良，科学合理利用草原资源，建立人工草地和牧草种子繁育基地，开发草产品；建立多元化的投入机制，培植草产业开发的龙头企业；进一步完善草地管理制度，全面彻底地落实草原有偿承包责任制；建立信息网络体系，把信息网络纳入畜牧业基础设施建设范围内，建立健全草畜产品信息网络。     

Biological characteristics of JAK2 transduced CD34+ cells from cord blood during ex vivo expansion

AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34⁺ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F₂JAK2).CD34⁺cells were enriched from cord blood with a MiniMACS system.The purified CD34⁺ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34⁺ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34⁺ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33⁺,CD61⁺ and Gly-A⁺ partial positive;CD38⁺ and HLA-DR⁺ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34⁺ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy.     

饲用植物的民族植物学--内蒙古阿鲁科尔沁旗的研究案例

论述了原住民利用饲用植物资源的传统知识和经验的民族植物学研究价值，提出了我国饲用植物资源的民族植物学研究的主要内容，并以内蒙古阿鲁科尔沁旗的研究结果作了案例进行了深入的探讨。     

暗期间断在植物生长发育中的应用研究(英文)

暗期间断属于光周期调控技术,被广泛应用于植物的生长发育研究。通过对暗期间断的类型、应用和机理三个方面研究成果进行了综述,其中在暗期间断类型部分总结了光质、间断暗期的次数和间隔时间方面的研究;在暗期间断应用部分总结了在植物形态发生、生理和生物化学方面的相关研究。在此基础上,提出了暗期间断在分子生物学研究及森林资源保护中的应用前景。     

烘烤工艺对烟叶色素及主要化学成分的影响

以烤烟G80为材料,研究了不同烘烤工艺对叶片色素含量及主要化学成分的影响.结果表明:中温中湿烘烤工艺有利于烟叶内部物质的充分转化,促进上等优质烟叶的形成.     

Effects of STK15 overexpression on growth of human esophageal carcinoma cell line KYSE150

AIM:To investigate the effects of serine/threonine kinase 15 (STK15) overexpression on the growth of human esophageal squamous-cell carcinoma (ESCC) cell line KYSE150. METHODS:Recombinant pEGFP-C1-STK15 expression vector was transfected into KYSE150 cells using LipofectamineTM 2000 and the expression of STK15 was detected by fluorescence microscopy and Western blotting. The proliferation of the cells in vitro was measured by MTT assay. The cell cycle distribution and apoptosis were detected by flow cytometry. The proliferation of the cells in vivo was measured by tumorigenicity experiment in nude mice. RESULTS:After recombinant pEGFP-C1-STK15 expression vector was stably transfected into KYSE150 cells, GFP-STK15 fusion protein localized to centrosome and spindle. The STK15-overexpressing colonies were further confirmed by Western blotting. MTT assay showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). Flow cytometry analysis showed that the percentage of the cells in G0/G1 phase and the cell apoptosis in STK15 overexpression group were decreased compared with control group (P<0.01). The tumorigenicity experiment in nude mice showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). CONCLUSION: Overexpression of STK15 in human ESCC KYSE150 cells promotes the cell growth in vitro and in vivo, indicating that STK15 may serve as a novel therapeutic target for esophageal carcinoma.     

棉花铃重AFLP标记的初步研究

本文以陆地棉铃重近等基因系为材料(大铃≥5.4g,小铃≤1.7g),构建大小铃两个近等基因池.利用AFLP标记技术对两基因池进行分析,64对引物组合共产生3840条稳定、清晰扩增带.五条扩增带在两基因池间呈现多态性,其中四条差异带出现在大铃基因池,编号分别为LB-1、LB-2、LB-3和LB-4;一条出现在小铃基因池,编号为SB-1.模板浓度梯度分析表明,在一定范围内,酶切模板浓度对AFLP结果影响较小.利用稍作修改的CTAB法提取的棉花DNA可用于AFLP分析.     

伊犁马体尺年龄对其竞赛性能影响的研究

对伊梨马体尺、年龄与不同赛程成绩关系的研究表明:体高对各赛程影响极显著(P<0.01),但随赛程增长,影响逐渐减弱;胸围和管 围对各赛程影响显著(P<0.05);年龄对长赛程影响显著(P<0.05)。