用CODEHOP设计简并引物克隆反刍月形单胞菌K6乙酸激酶基因片段

利用CODEHOP设计细菌乙酸激酶的简并引物,选用1对引物ACKSe以高效丙酸生成菌反刍月形单胞菌K6基因组DNA进行PCR,得到749 bp PCR产物,产物经pMD18-T载体克隆转化至DH5α大肠杆菌中,测序后经Blastx比对,此DNA产物与其他菌属来源的乙酸激酶蛋白序列具有相似性,所克隆的序列即为K6的乙酸激酶基因片段。用CODEHOP程序化设计的简并引物可信性强,阳性率高。该基因的成功克隆为丙酸生成菌K6乙酸代谢工程研究提供了依据。     

高校管理体系的构建与教学质量的提高

当前,高校管理体系是健全高校运行过程中不可或缺的一部分,是提高教育教学质量的重要保障.对教育在管理过程中存在的问题进行分析,构建高校管理体系的合理模式,从而提高高校教育教学质量.     

锡林郭勒草原景观动态数据库及信息服务系统的建立

利用ASP技术结合SQL Server 2000数据库、web技术设计开发了具有浏览、检索、维护和添加功能的锡林郭勒草原景观的动态数据库及信息服务系统。完成了锡林郭勒草原草地景观动态监测的各种指标、数据、图像及其信息化管理,并将信息以图文并茂的形式直观地展现在用户面前。     

Effect of heme oxygenase on vascular remodeling in renal hypertension

AIM: To investigate the effect of heme oxygenase on vascular remodeling in renal hypertension. METHODS: Male Wistar rats were randomly divided into sham-operated, 2K1C （two-kidney one-clip） and hemin-induced groups. Four weeks after the treatments, the thickness of aortic media and HO enzymatic activity of the aorta were determined. Immunohistochemical staining was carried out to detect protein of HO-1 in the aorta. RESULTS: The blood pressure in 2K1C renal hypertension rats started to increase two weeks after the surgery and stabled at a high level at the 4th week. Hemin, an inducer of HO-1, markedly inhibited the increase in blood pressure. Aortic medium thickness of the 2K1C rats at 4th week was 27.5% thicker than that in the sham-operated rats. The thickness of aortic medium of the hemin-induced rats was 16.1% less than that in 2K1C group. At the 4th week after operation, protein level and enzymatic activity of HO-1 in aorta were higher than that in 2K1C group compared to those in the sham-operated group. CONCLUSION: Renal hypertension caused vascular remodeling and the activation of HO-1. HO-1 induction decreased the blood pressure of renal hypertension and reduced vascular remodeling.     

广东鹤山南亚热带丘陵人工林昆虫资源的研究（Ⅰ）

本文根据2001年和2002年的调查结果，记述了广东鹤山南亚热带丘陵退化生态系统恢复中四种人工林的昆虫种类，共计14目161科488种，本文记载11目84科216种。     

Effects of 4-hydroxytamoxifen on the growth and proliferation of prolactinomas CH3 cells

AIM: To investigate the effect of estrogen antagonists on the in vitro growth of human prolactinomas. METHODS: RT-PCR was applied to the detection of estrogen receptor (ER) mRNA expressed in a human prolactinomas CH3 cell strain. Estradiol and 4-hydroxytamoxifen (OHTam) were added respectively at different concentrations into the culture medium. Cell number and levels of ER mRNA were examined. RESULTS: The growth of CH3 cells became slower in estrogen-deprived medium than that in nomal culture and was higher in medium containing estrogen(E2) at concentration of 10^-8 mol/L than at concentration of 10^-6 mol/L. OHTam (10^-6mol/L) inhibited the growth of CH3 cell strain treated with E2. The expression of ER mRNA in CH3 cells was observed, the levels of ER mRNA in the E2 (10^-8mol/L) group, higher than those in estrogen deprived group. OHTam (10^-6mol/L) obviously inhibited the expression of ER mRNA. CONCLUSION: The growth of CH3 cells depends on estrogen, estrogen antagonists inhibits the growth of CH3 cells and decline the levels of ER mRNA. ER levels in human prolactinomas cell lines can be auto-regulated.     

GX实验是实施数学素质教育的成功范例

认为GX实验取得了很大成功. 其教学符合数学素质教育的全体性、全面性、主体性、基础性、发展性特征; 能有效培养学生的数学认知与方式、多种数学能力、数学意识与观念; 能促进学生情感、态度和个性品质的发展; 实现了数学素质教育的基本目标, 具有较强的可操作性、可接受性和可迁移性, 是数学素质教育的重要实践与成功范例. GX实验对目前我国基础教育数学课程改革具有启发与借鉴意义.     

桉属植物叶片DNA抽提

对植物组织DNA抽提是在分子水平上对植物进行系统分析与研究的基础性工作。我们使用改良CTAB法抽提桉属植物叶片中总的DNA获得了成功，其纯度和得率完全能满足常规分子生物学操作的要求。与DNA得率有关的几个问题在此一并探讨。