如皋火腿酯类物质氧化和水解分析

为了研究如皋火腿中的酯类物质。选取7条4.5~5.0 kg如皋火腿为试材,用常规方法测定其理化指标(水分、盐分和pH值),通过气相色谱测定从提取脂质中分离的游离脂肪酸含量,并测定其共轭二烯值与羰基值。在如皋火腿股二头肌和皮下脂肪中含盐量为11.60%和1.07%,水分为40.21%和3.65%,pH值为5.71和6.02,游离脂肪酸含量为49.65%和26.11%,除pH外均存在极显著差异。如皋火腿中含有丰富的游离脂肪酸,在皮下脂肪中油酸的含量最多,股二头肌中亚油酸的含量最多。火腿中存在少量的反式脂肪酸,皮下脂肪有反式的油酸,股二头肌中有反式油酸、亚油酸和亚麻酸。股二头肌的氧化明显高于皮下脂肪,可能与酶活力、氧化方式有关。     

中国农业大学校内文献资源共享机制研究

随着高等院校招生规模的扩大和信息资源更新速度的加快，生均可利用文献的拥有量并没方显著增加，文献的供给与需求之间出现了较大的缺口，而校内文献资源不能有效的共知共享也是可用文献资源不能满足需求的原因之一。本文通过对中国农业大学院系资料室现有资源与服务现状的调查分析，阐明了校内文献资源共享的必要性，提出了建立校内文献资源共享机制的建议。     

高纬度地区食用仙人掌无公害栽培技术

食用仙人掌,又名米邦塔,属仙人掌类植物,原产于美洲、非洲,既可作为观赏性植物,又可食用和药用。肉质绿色,有节无刺或基本无刺,茎节为扁平状,呈卵形,长14~45cm,株高2~3m。据测定,食用仙人掌的钾、钙、铜、铁、锰、黄酮类物质和多糖含量丰富,经常食用对高血糖、高血脂、高血压和高胆固醇有良好的疗效。食用仙人掌已成为人们日常生活中不可缺少的特色蔬菜和水果。我国南北各地20世纪90年代后期,北京、福建、黑龙江等地均开始了食用仙人掌的种植和生产销售。哈尔滨还出现了专门经营墨西哥仙人掌特色菜肴的酒店和快餐店。食用仙人掌产量一般在60…     

齐口裂腹鱼端脑形态和组织学研究

采用苏木精 -伊红、美兰染色技术对 5尾齐口裂腹鱼端脑的外部形态和组织结构进行了研究。观察发现 ,齐口裂腹鱼的端脑发达 ,由嗅球、嗅茎和左右端脑半球构成。嗅球位于嗅窝内 ,嗅囊的后方 ,以嗅茎与后面的左右端脑半球联系。嗅球的组织结构成层状结构 ,从外向内依次为上皮、神经纤维层和小细胞层。端脑半球外部被有原脑皮 ,两者之间的腔隙为脑室 ,其内的纹状体被横行和纵行的沟分成若干小叶。神经核分布于纹状体的周缘 ,主要有连前核、背嗅核、侧嗅核、视前核、梨状核、侧连核和脚内核等。本文对纹状体内各核的特征进行观察和描述。     

Web农业古汉语电子词典设计

简要介绍了Web农业古汉语电子词典项目缘起和设计方案,包括数据库设计、Web界面设计、数据库检索方法设计、古汉语词汇收集和释义等。项目完成后,可为读者阅读农业古籍提供一个实用的辅助工具。     

广东省甘蔗品种区试中基因型与环境互作分析

针对广东省2004-2005年甘蔗品种区域试验数据,应用Tai氏方法分析基因型与环境交互效应的结果表明,粤糖96-86高产、适应性强、稳定性好,居于首位;粤糖95-168和ROC22高产但适应性较低,粤糖96-24、粤糖97-64和ROC10低产但适应性强,粤糖96-177产量低而且适应性差,它们的稳定性表现不一.     

荧光原位杂交(FISH)技术在转基因小鼠检测中的应用

制备转基因小鼠特有的DIG标记探针，应用染色体荧光原位杂交（FISH）技术，对转基因小鼠外周血细胞的遗传性状进行分析，检测其合子类型，以挑选纯合子小鼠用于保种。结果杂合型小鼠61％的细胞有一个荧光信号，而纯合型小鼠约24％的细胞有两个荧光信号，48％的细胞有一个荧光信号，野生型小鼠无荧光信号。因此染色体荧光原位杂交技术可作为检测转基因小鼠遗传特性的一种方法。     

青虾人工繁殖研究初报

青虾怀卵量与个体大小呈正相关 ,相对怀卵量在 6 0 0— 70 0粒 / g之间 ;统计出青虾雄雌性比在繁殖早期为 1.10 7:1、产卵盛期为 1.4 39:1;同时观察记录了青虾生殖蜕皮、交配、产卵、孵化全过程。     

Production and Identification of High Affinity Monoclonal Antibodies Against Pesticide Carbofuran

To produce high-affinity monoclonal antibodies against pesticide carbofuran, and the develop immunochemical assays for people＇s health and environmental protection, the hapten 4-[[（2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy） carbonyl]- amino]-butanoic acid （BFNB） of carbofuran was synthesized and Balb/c mice were immunized by the hapten-carrier （BFNB-bovine serum albumin, BFNB-BSA） conjugates. The splenocytes of immunized mice were fused with Sp2/0 cells and the cultural supernatants of hybridoma cells were screened by the indirect enzyme-linked immunoabsorbent assay （ELISA）, based on BFNB-ovoalbumin conjugates （BFNB-OVA）. Purified monoclonal antibody （McAb） was obtained from fluids of ascites, deposited by octanoic acid and ammonium sulfate. The affinity and the specificity of McAb were characterized by ELISA or indirect competitive ELISA. A hybridoma cell line （5D3） secreting anti-carbofuran McAb had been established. The titer of culture medium and ascites was up to 1：2.048 × 10^3 and 1：1.024 × 10^6, respectively, and the subtype of the McAb was IgG1. The affinity constant of the McAb was about 2.54 × 10×9 L mol^-1, with an IC50 value of 1.18 ng mL^-1 and a detection limit of 0.01 ng mL^-1. Cross-reactivity studies showed that the McAb was quiet specific for carbofuran, as among the four analogous compounds, they were all hardly recognized （4.59 × 10^-4% for 2,3-dihydro-2,2- dimethyl-7-benzofuranol and less than 3.0 × 10^4% for others）. The prepared McAb had a very high affinity and specificity, and it could be used to develop ELISA for rapid determination of carbofuran.     

Water Availability for Winter Wheat Affected by Summer Fallow Tillage Practices in Slope Dryland

The tillage experiments for winter wheat were conducted on the slope farmland in Luoyang,Henan Province in the semihumid to arid loess plateau areas of North China. Different tillage methods inclu-ding reduced tillage (RT), no-till (NT), 2 crops/year (2C), subsoiling(SS), and conventional tillage (CT)were compared to determine the effects of tillage methods on soil water conservation, water availability, andwheat yields in a search for better farming systems in the areas. The NT and SS showed good effects on waterconservation. The soil water storage increased 12 - 33 mm with NT and 9 - 24 mm with SS at the end of sum-mer fallow periods. The soil evaporation with NT and SS decreased 7 - 8 mm and 34 - 36 mm during the fallowperiods of 1999 and 2001, respectively. Evapotranspiration (ET) with NT and SS increased about 47 mm dur-ing wheat growth periods of 2000 to 2001. Treatment RT and 2C had low water storage and high water lossesduring the fallow periods. The winter wheat yields with conservation tillage practices were improved in the 2ndyear, increased by 3, 5 and 8 % with RT, NT and SS, respectively, compared with CT. The highest wheatyields were obtained with subsoiling, and the maximum economic benefits from no-till. All conservation tillagepractices provided great benefits to saving energy and labors, reducing operation inputs, and increasing eco-nomic returns. No-till and subsoiling have shown promise in increasing water storage, reducing water loss, en-hancing water availability, and saving energy, as well as increasing wheat yield.