全文获取类型
收费全文 | 13588篇 |
免费 | 777篇 |
国内免费 | 1143篇 |
专业分类
林业 | 941篇 |
农学 | 681篇 |
基础科学 | 494篇 |
1280篇 | |
综合类 | 6961篇 |
农作物 | 952篇 |
水产渔业 | 599篇 |
畜牧兽医 | 1988篇 |
园艺 | 1034篇 |
植物保护 | 578篇 |
出版年
2024年 | 99篇 |
2023年 | 222篇 |
2022年 | 533篇 |
2021年 | 531篇 |
2020年 | 493篇 |
2019年 | 543篇 |
2018年 | 356篇 |
2017年 | 618篇 |
2016年 | 404篇 |
2015年 | 645篇 |
2014年 | 668篇 |
2013年 | 815篇 |
2012年 | 1161篇 |
2011年 | 1243篇 |
2010年 | 1211篇 |
2009年 | 1105篇 |
2008年 | 1059篇 |
2007年 | 916篇 |
2006年 | 824篇 |
2005年 | 625篇 |
2004年 | 415篇 |
2003年 | 245篇 |
2002年 | 233篇 |
2001年 | 224篇 |
2000年 | 178篇 |
1999年 | 53篇 |
1998年 | 11篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1987年 | 7篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1981年 | 1篇 |
1965年 | 1篇 |
1962年 | 5篇 |
1960年 | 1篇 |
1956年 | 6篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 10 毫秒
101.
XIAO Xiao-ping LI Yue-qin XU Bin LI Hong-jian ZHANG Xin ZHOU Tian-hong ZOU Yi 《园艺学报》2009,25(7):1254-1261
AIM: To study the anti-proliferation effect of overexpressed SSTR2 in experimental cancer with different profiles of endogenous SSTRs expressions and the possible signaling pathways involved. METHODS: In the first experiment, the growth of the tumor xenografts of the inoculated capan-2 cells and A549 cells overexpressing SSTR2 or LacZ was investigated in nude mice. In the second experiment, the adenoviral vector expressing SSTR2 were introduced into experimental capan-2 xenografts by intratumoral injection. The growth inhibition of these experiment tumors was observed and the potential influences on different signaling pathways were analyzed by immunoassays. RESULTS: Overexpression of SSTR2 inhibited the growth of tumors with different profiles of endogenous SSTRs, including experimental capan-2 xenografts that had endogenous SSTR2 expression. Overexpression significantly affected a number of components in apoptotic pathway, MAPK pathway and angiogenesis. CONCLUSION: SSTR2 is a promising candidate for gene therapy in a wide spectrum of cancers. 相似文献
102.
WAN Jian-xin GUO Qi WANG Jing-han PAN Yang-bin CUI Jiong FU Bin-bin XU Yan-fang 《园艺学报》2009,25(8):1628-1634
AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory. 相似文献
103.
4甜樱桃栽培技术
4.1建园
4.1.1园址的选择
与其他果树相比。甜樱桃对生态条件的要求较高。它不抗寒,不耐瘠薄,抗涝性差,怕干旱,忌大风。花期早而易遭霜冻,喜光性强。因此,应选择土壤比较肥沃、土层较深厚、中性或微酸性壤土及沙壤土, 相似文献
104.
105.
【目的】水稻种子主要以淀粉形式储藏能量。淀粉合成需要多种酶类和调控因子参与,机制较为复杂。本研究利用水稻胚乳发育缺陷突变体,克隆和鉴定新的调控淀粉合成相关基因,旨在为研究淀粉合成及其调控提供理论依据。【方法】从化学诱变剂甲基亚硝基脲(1-methyl-1-nitroso-urea, MNU)处理的宁粳3号(Ningjing 3, WT)突变体库中筛选到一个能稳定遗传的胚乳粉质皱缩突变体,命名为fse4 (floury and shrunken 4 )。与籼稻品种Dular杂交获得F1种子(F2),通过图位克隆的策略确定FSE4候选基因。利用杂合植株(FSE4fse4)分离出的粉质种子,观察形态学特征,分析其理化性质。使用扫描电镜和半薄切片技术观察胚乳结构。使用qRT-PCR和免疫印迹分析淀粉合成相关基因表达模式和淀粉合成相关酶类的蛋白积累量。利用全自动氨基酸分析仪测定成熟胚乳各氨基酸含量。【结果】突变体fse4籽粒宽度、厚度以及千粒重显著下降,同时胚乳中总淀粉、总蛋白、直链淀粉含量亦显著下降,而脂肪含量显著上升;淀粉黏度、崩解值和消减值显著低于野生型。突变体fse4中多为单粒型淀粉颗粒,且排列分散。FSE4定位于第5染色体长臂约252 kb的区间内,测序发现编码Δ1-吡咯啉-5-羧酸合成酶基因 (Delta 1-pyrroline-5-carboxylate synthetase, P5CS)第1外显子上发生单碱基替换,导致一保守的氨基酸发生变异。突变体fse4中大部分淀粉合成相关基因表达量下调,多种淀粉合成相关蛋白积累量减少。突变体fse4米粉中多种氨基酸含量发生显著变化,游离氨基酸含量是其野生型的3.6倍。此外,外源喷施脯氨酸能部分恢复突变体fse4种子萌发缺陷表型。【结论】FSE4编码脯氨酸合成关键限速酶P5CS,该基因对胚乳中氨基酸的合成及代谢起重要的调控作用,并影响淀粉的合成与积累。 相似文献
106.
低磷胁迫对不同水稻品种叶片膜脂过氧化及保护酶 总被引:33,自引:1,他引:33
利用砂培方法,研究了耐低磷水稻品种(大粒稻、莲塘早3号)和低磷敏感水稻品种(沪占七、新三百粒)在低磷胁迫下,水稻叶片膜脂过氧化和保护酶活性的变化。研究结果表明,低磷胁迫使水稻叶片膜脂过氧化加剧,低磷敏感水稻品种的叶片膜脂过氧化程度高于耐低磷水稻品种;耐低磷品种叶片中SOD、CAT和POD活性在低磷胁迫期间相对稳定,而低磷敏感品种在低磷胁迫初期均有明显增加,尔后又快速下降,这表明保护酶的绝对活性与低磷胁迫无关,而变化趋势与低磷胁迫有关。 相似文献
107.
108.
LIAi-hong XuXin-ping DAIZheng-yuan CHENZong-xiang LIBao-jian ZHANGHong-xi PANXue-biao 《水稻科学》2004,11(3):95-100
Resistance of forty-one homozygous rice lines transformed with chitinase gene (RC24) and β-1,3 -glucanse gene β-1,3-Glu) to sheath blight was analyzed by inoculation. Among different lines, the resistance had significant differences according to the result by cluster analysis. The lines could be categorized into resistant, moderately resistant, moderately susceptible and susceptible types, while 92.1% of which belonging to moderately resistant or moderately susceptible type. For different resistant or susceptible lines, the resistance to rice sheath blight was remarkable correlated with the chitinase activity of transgenic lines except resistant type lines, in which enzyme activity coded by target gene was lower than moderately resistant type. The chitinase activity of transgenic lines tested at different time after inoculation or different organs of the same plant was uniform, which suggested that the expression of chitinase gene was constitutive in nature. Check varieties chitinase activity would change at different time after inoculation and reach a peak at sometime, but it had no difference at various pans of the same plant. 相似文献
109.
110.
Lu Zhong-xian S. VILLAREAL Yu Xiao-ping K. L. HEONG HU Cui 《水稻科学》2006,13(3):218-226
Biodiversity both theoretically and practically has relevance in addressing many problems of contemporary agriculture and allows the formation of functional groups that drive key ecosystem processes [1]. One of the most important processes in agroecosystems is pest regulation, because biodiversity is related closely to host-plant resistance, pest management attributes, natural biological control agents and their impacts, and stability as the ecological basis for pest management [2-3]. On one h… 相似文献