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741.
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The percentage, distribution, shape, intensity of staining and morphometrical parameters of the pituitary TSH immunoreactive cells and the histological features of the thyroid glands, were compared between adult rats with intact adrenals, without adrenals and biadrenalectomized animals with neonatal adrenal grafts. After the removal of the adrenal glands, TSH immunoreactive cells increased in percentage and exhibited a higher complexity of the cellular outline, than that of the intact animals. The nuclear, cytoplasmic and cell areas were significantly increased. However the bigger enhancement of the cytoplasmic area relative to the nuclear area, produced a decrease in the nuclear/cell area ratio. The thyroid glands showed some histological evidences of activation. After the transplantation of neonatal adrenal glands to adult rats, several adrenocortical nodules were present in the lumen of the small bowel segment. These adrenal masses induced a great decrease in the TSH cell area, which coupled with a smaller but significant variation of the nuclear area, led to an increase in the nuclear/cell area ratio relative to that observed in adrenalectomized animals. In addition, the distribution, shape and intensity of the immunoreactive material was similar to that observed in intact animals. In this experimental group, thyroid histology was observed to be similar to that of the intact animals. 相似文献
744.
A serological study of canine parvovirus (CPV) infections in a colony of dogs was conducted over a period of 8 months. Twenty-two of 24 adult dogs sampled initially had significant antibody titres to CPV. Nine litters of puppies wer e bled at fortnightly intervals and the sera tested for antibodies to CPV. Twenty-nine of 35 natural CPV infections observed were subclinical. A puppy in one litter developed vomiting and diarrhoea during the same period as seroconversion to CPV. Four puppies from a further litter developed histologically confirmed myocarditis. Serological testing of this latter litter indicated that the virus infections occurred between 2 weeks prior to and 1 week post whelping, and that clinical disease developed 20–40 days after viral infection. The mean half life of the decline of passively derived immunity to CPV was 8.3 days. 相似文献
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747.
S R Ostrowski B P Smith S J Spier B B Norman M N Oliver 《Journal of the American Veterinary Medical Association》1989,195(4):481-484
Cattle that had recovered from clinical oak bud toxicosis (as assessed by normal BUN and serum creatinine values and good appetite) performed better than did clinically normal herdmates when weight gain and feed efficiency were compared. Both groups had a rate of weight gain (oak bud-exposed cattle, 1.76 kg/steer/d; control cattle, 1.57 kg/steer/d) that exceeded the feedlot mean value (approximately 1.4 kg/d) for cattle (of similar starting weight and diet) that had zeranol implants, and the difference was significant (P less than 0.005) for both groups. The difference in weight gain performance of oak bud-exposed cattle, compared with control cattle, was not highly significant; however, the improved feed efficiency was significantly (P less than 0.05) better than that in controls (2.57 kg of feed/kg of weight gain vs 3 kg of feed/kg of weight gain). On the basis of accepted criteria (increased rate of weight gain and efficiency of feed conversion), the group of oak bud-exposed cattle of this study had compensatory weight gain. Thus, cattle with good evidence of clinical recovery from oak bud toxicosis can be expected to perform in the feedlot at least as well as cattle not exposed to oak bud toxicosis, and may have compensatory weight gain. 相似文献
748.
Frank LA Schmeitzel LP Oliver JW 《Journal of the American Veterinary Medical Association》2001,218(2):214-216
OBJECTIVE: To evaluate adrenal sex hormone concentrations in neutered dogs with hypercortisolemia. DESIGN: Case series. ANIMALS: 11 neutered dogs with hypercortisolemia. PROCEDURE: Serum samples obtained before and 1 hour after administration of ACTH were evaluated for concentrations of cortisol, progesterone, testosterone, dehydroepiandrosterone sulfate or androstenedione or both, and 17-hydroxyprogesterone. RESULTS: For all dogs, concentrations of 1 or more adrenal sex hormones were substantially greater than reference range values before or after administration of ACTH. Testosterone concentration was not greater than reference range values in any of the dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Results emphasize the importance of ruling out hypercortisolemia before measuring adrenal sex hormone concentrations as a means of diagnosing adrenal hyperplasia syndrome (alopecia X) in dogs. 相似文献
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Almeida RA Oliver SP 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(10):759-763
We reported previously that pre-incubation of Streptococcus uberis with collagen induced expression of S. uberis surface proteins. In a subsequent study, we showed that incubation of S. uberis with extracellular matrix proteins, particularly collagen, increased adherence and internalization of S. uberis to mammary epithelial cells. In the present report, the potential mechanism by which S. uberis exploits the presence of collagen to enhance adherence to bovine mammary epithelial cells was evaluated. Adherence assays were conducted with S. uberis pre-treated with and without collagen and co-cultured in medium supplemented with or without collagen. Pre-incubation with collagen followed by co-culture in medium containing collagen up-regulated ligands that enhanced adherence of S. uberis to mammary epithelial cells. Collagen-up-regulated ligand(s) also increased adherence of S. uberis to mammary epithelial cells in the absence of collagen, but adherence was lower than when collagen was present during the adherence assay. Chloramphenicol was added to the culture medium to inhibit bacterial protein synthesis. Adherence decreased significantly in chloramphenicol-treated S. uberis pre-treated or co-cultured in the presence of collagen. These results suggest that S. uberis expresses ligands with affinity for collagen that are up-regulated by collagen. We hypothesize that these ligands increase adherence by using collagen as a bridge between the bacterium and host cell and/or by direct interaction with host cell receptor(s). 相似文献