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71.
T Matsuno F Hariguchi T Okamoto 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(1):13-17
Pamaquine and primaquine, the well known antimalarial 8-aminoquinolines, have not been reported for their anticoccidial activity. A series of battery experiments was conducted to investigate their activity against a laboratory strain of Eimeria tenella, E. necatrix, E. acervulina, E. maxima, or E. brunetti and revealed that both drugs were effective against E. tenella and E. necatrix, but not against the other three species. Pamaquine suppressed the symptoms of E. tenella induced coccidiosis at concentrations above 125 ppm in feed and primaquine controlled the clinical signs as well at levels above 31.2 ppm. The activity against E. necatrix was observed with pamaquine at 250 ppm and with primaquine at levels above 125 ppm. Pamaquine showed a tendency apparently to reduce body weight gain at 125-500 ppm, whereas primaquine showed the same tendency at 500 ppm. In a concomitantly conducted experiment, this adverse effect of pamaquine was averted in its molecular complexes with benzophenone, nitropyrazole, dinitrobenzoic acids and quinoline, and in its salts of sulfate or zinc chloride, and yet these compounds retained the same anticoccidial activity as of pamaquine. This suggests that these compounds had the broadened safety margin. Judging from their susceptibility to these compounds. E. tenella and E. necatrix will have similar metabolic functions to those of blood cell parasitizing protozoa like plasmodia and prioplasma, which are easily suppressed by this class of compound. 相似文献
72.
Y Akihara Y Shimoyama K Kawasako M Komine K Hirayama A Terasawa T Ohmachi K Matsuda M Okamoto H Taniyama 《Reproduction in domestic animals》2007,42(5):495-501
In the present study, 15 canine ovaries without morphological lesions were examined histologically and immunohistochemically by using a large number of proteins including AE1/AE3, cytokeratin7 (CK7), CK13, CK20, vimentin, desmin, alpha smooth muscle actin (alphaSMA), calponin, S100, Neurofilaments, Inhibinalpha, placental alkaline phosphatase (PLAP) and neuron-specific enolase. Ovarian structures observed in this study included surface epithelium (SE), cortical tubules (CT), tunica albuginea (TA), stromal cells (SC), internal endocrine cells (IE), rete ovarii (RO) and fallopian tubes (FT). SE, CT, RO and FT were broadly immunoreactive for desmin. Besides AE1/AE3 and vimentin, desmin was also closely linked to these structures. Rete ovarii forming a reticular structure showed a positive reaction to S100. Surface epithelium was immunoreactive for PLAP at a significantly high level. In conclusion, these results indicate a specific segment of immunoreactivity as well as the broad range of immunoreactivity in canine ovary. The distinct patterns of immunoreactive for various kinds of proteins will play an important role in facilitating their identification and discrimination even in a normal canine ovary with a complex structure. 相似文献
73.
74.
Chuma T Ikeda T Maeda T Niwa H Okamoto K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):1027-1029
Cecal contents (16 samples/each flock) of broilers derived from 212 flocks were investigated for colonization of Campylobacter from 1995 to 1999 in the southern part of Japan, and the isolates were tested for antimicrobial susceptibilities. C. jejuni-positive flocks numbered 42 (19.8%) and C. coli-positive ones 26 (12.3%); Campylobacter spp. were recovered from 68 flocks (32.1%) in total. MICs of ampicillin, erythromycin (EM), tetracycline, nalidixic acid (NA), norfloxacin (NFLX), and ofloxacin (OFLX) to these 68 Campylobacter isolates were determined. Quinolone-resistant Campylobacter isolates numbered 22 (32.4%). All the isolates except one were cross-resistant to NA, OFLX, and NFLX. A high frequency of quinolone-resistance was found in both C. jejuni and C. coli, whereas a high level of EM-resistance was found in only C. coli strains. All C. jejuni isolates were sensitive to EM. 相似文献
75.
76.
Soliman M Okamoto S Kitamura H Kushibiki S Kimura K Saito M 《The Japanese journal of veterinary research》2004,52(2):95-99
The responses of plasma cortisol and adrenocorticotropic hormone (ACTH) were examined to intravenous injection of recombinant bovine tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (INF-gamma) in Holstein cows. INF-gamma induced dose-dependent rises in the plasma levels of both cortisol and ACTH, while TNF-alpha induced comparable plasma cortisol responses with much smaller rises in plasma ACTH. The results suggest a direct stimulatory action of TNF-alpha on cortisol secretion from the adrenal gland in cattle. 相似文献
77.
78.
Nitric oxide (NO) serves as a signal in plants. An Arabidopsis mutant (Atnos1) was identified that had impaired NO production, organ growth, and abscisic acid-induced stomatal movements. Expression of AtNOS1 with a viral promoter in Atnos1 mutant plants resulted in overproduction of NO. Purified AtNOS1 protein used the substrates arginine and nicotinamide adenine dinucleotide phosphate and was activated by Ca2+ and calmodulin-like mammalian endothelial nitric oxide synthase and neuronal nitric oxide synthase, yet it is a distinct enzyme with no sequence similarities to any mammalian isoform. Thus, AtNOS1 encodes a distinct nitric oxide synthase that regulates growth and hormonal signaling in plants. 相似文献
79.
Sawabe A Morita M Kiso T Kishine H Ohtsubo Y Ouchi S Okamoto T 《Journal of agricultural and food chemistry》1999,47(2):588-593
New polyisoprenepolyols (hypsiziprenol AA and BA) were isolated from an edible mushroom (Hypsizigus marmoreus). These polyols occur as a mixture of homologous polyisoprene derivatives with 40-70 carbon atoms. Analyses by FAB/MS in the positive and negative ion modes are complementary with each other in that the former provides information on the number of hydroxy groups present while the latter specifies the isoprenoid sequence, and thus become a powerful tool for analyzing the structures of polyisoprenepolyols. No polyisoprenepolyols obtained here were found to have antitumor activity on NCI-H292 and EL-4 cell lines. 相似文献
80.
Malathion was incubated in water extracts of vegetables at various temperatures and pH, and the amount of malathion present over time was analyzed by a gas chromatograph with a flame photometric detector. Malathion was degraded to a nondetectable level in a 1% asparagus extract incubated at pH 7.4 and 37 degrees C for 4 h. Carrot extract showed the second highest rate of malathion degradation (76%), followed by kale extract (23.7%), spinach extract (9.7%), and broccoli extract (1.5%) under the same conditions. The highest degradation rates of malathion were observed at 37 degrees C, when three different temperatures were tested (5, 25, and 37 degrees C) at pH 7.4. Rate constants were 0.134 min(-)(1) from a 1% asparagus solution and 0.095 min(-)(1) from a 0.5% asparagus solution. The highest degradation rate of malathion was achieved at pH 9 among the pHs tested (pH 4, 7.4, and 9) in a 0.5% asparagus solution. The 0.5% asparagus solution degraded dicarboxylic acid esters by almost 100% for dimethyl succinate and diethyl adipate, by 64% for diethyl acetyl succinate, and 30% for diethyl benzyl malonate when incubated at pH 9 for 20 min. The results support the hypothesis that the enzyme that degrades malathion in the asparagus solutions is a carboxylesterase. 相似文献