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Schukken YH González RN Tikofsky LL Schulte HF Santisteban CG Welcome FL Bennett GJ Zurakowski MJ Zadoks RN 《Veterinary microbiology》2009,134(1-2):9-14
In this paper, we analyzed a very large field data set on intramammary infections (IMI) and the associated somatic cell count (SCC) in dairy cows. The objective of the study was to analyze the impact of coagulase-negative staphylococci (CNS) IMI on cow SCC, both mean and variability, and on the potential of these infections to have a major impact on the bulk milk SCC (BMSCC). Data and milk samples for bacterial culture were collected by Quality Milk Production Services (QMPS) between 1992 and March of 2007. The QMPS program services dairy farms in New York State and other states in the Northeastern USA and operates in conjunction with Cornell University. Only records from cows where SCC and milk production data were available, and where only one organism was isolated from bacterial cultures of milk samples (or where culture was negative) were used for this analysis. A total of 352,614 records from 4200 whole herd mastitis screening sampling qualified for this study. Within herds an average of 15% (S.D. 12%) of cows sampled were infected with CNS, ranging between 0 and 100%. Average within herd prevalence of cows with a CNS IMI and an SCC over 200,000 cells/ml was 2% (S.D. 4%) with a minimum of 0% and a maximum of 50%. Results of linear mixed models showed three distinct populations of IMI statuses: negative cultures with the lowest SCC; CNS and Corynebacterium bovis with a moderate increase in SCC, and Streptococcus agalactiae, Streptococcus spp. and Staphylococcus aureus showing an important increase in SCC. Surprisingly, milk production was slightly but significantly higher in CNS infected cows compared to culture-negative cows, whereas it was strongly reduced in cows with a major pathogen IMI. The percentage contribution of CNS infections to the BMSCC was 17.9% in herds with a BMSCC less than 200,000 cells/ml. This value decreased to 11.9 and 7.9% in herds with bulk milk SCC between 200,000 and 400,000 and over 400,000 cells/ml, respectively. We concluded that very few herds with milk quality problems would have an important increase in BMSCC that could be mostly attributed to CNS infections. On the other hand, in herds with low BMSCC, CNS infections may be an important contributor to the total number of somatic cells in the bulk milk. 相似文献
23.
Blagburn BL Dryden MW Payne P Rust MK Jacobs DE Bond R Hutchinson MJ Denholm I Mehlhorn H Vaughn M Mencke N Schroeder I Hostetler J Endrizzi M 《Veterinary therapeutics : research in applied veterinary medicine》2006,7(2):86-98
A flea larval bioassay was developed by an international team of scientists to monitor the susceptibility of fleas (Ctenocephalides felis) to imidacloprid (Advantage, Bayer HealthCare). The assay was validated using laboratory and field isolates of C. felis. Flea eggs representing different field isolates of C. felis were collected by veterinarians in the United States, United Kingdom, and Germany. Of the 972 flea isolates obtained during the 5-year study, 768 contained sufficient numbers of eggs to conduct the larval bioassay. Greater than 5% survival occurred for only six of the field isolates evaluated. Further evaluation and analysis of these isolates demonstrated that they did not differ significantly in their susceptibility to imidacloprid from the reference strains used to develop the assay. Collections of field flea isolates will continue in an attempt to detect and document any change in the susceptibility of field flea populations to imidacloprid. 相似文献
24.
Anders Michelsen Michael Andersson Annelise Kjøller 《Soil biology & biochemistry》2004,36(11):1707-1717
A thorough understanding of the role of microbes in C cycling in relation to fire is important for estimation of C emissions and for development of guidelines for sustainable management of dry ecosystems. We investigated the seasonal changes and spatial distribution of soil total, dissolved organic C (DOC) and microbial biomass C during 18 months, quantified the soil CO2 emission in the beginning of the rainy season, and related these variables to the fire frequency in important dry vegetation types grassland, woodland and dry forest in Ethiopia. The soil C isotope ratios (δ13C) reflected the 15-fold decrease in the grass biomass along the vegetation gradient and the 12-fold increase in woody biomass in the opposite direction. Changes in δ13C down the soil profiles also suggested that in two of the grass-dominated sites woody plants were more frequent in the past. The soil C stock ranged from being 2.5 (dry forest) to 48 times (grassland) higher than the C stock in the aboveground plant biomass. The influence of fire in frequently burnt wooded grassland was evident as an unchanged or increasing total C content down the soil profile. DOC and microbial biomass measured with the fumigation-extraction method (Cmic) reflected the vertical distribution of soil organic matter (SOM). However, although SOM was stable throughout the year, seasonal fluctuations in Cmic and substrate-induced respiration (SIR) were large. In woodland and woodland-wooded grassland Cmic and SIR increased in the dry season, and gradually decreased during the following rainy season, confirming previous suggestions that microbes may play an important role in nutrient retention in the dry season. However, in dry forest and two wooded grasslands Cmic and SIR was stable throughout the rainy season, or even increased in this period, which could lead to enhanced competition with plants for nutrients. Both the range and the seasonal changes in soil microbial biomass C in dry tropical ecosystems may be wider than previously assumed. Neither SIR nor Cmic were good predictors of in situ soil respiration. The soil respiration was relatively high in infrequently burnt forest and woodland, while frequently burnt grasslands had lower rates, presumably because most C is released through dry season burning and not through decomposition in fire-prone systems. Shifts in the relative importance of the two pathways for C release from organic matter may have strong implications for C and nutrient cycling in seasonally dry tropical ecosystems. 相似文献
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M Craddick R Patel A Lower S Highlander M Ackermann D McClenahan 《Veterinary immunology and immunopathology》2012,149(1-2):58-65
Mannheimia haemolytica, one of the agents associated with bovine respiratory disease complex, can cause severe lung pathology including the leakage of vascular products into the airways and alveoli. Previous work by this laboratory has demonstrated that bovine lung endothelial and epithelial cells undergo dramatic permeability increases when exposed to adenosine-5'-triphosphate (ATP). Therefore, we wanted to determine if ATP levels were elevated in bronchoalveolar lavage (BAL) samples from calves experimentally infected with M. haemolytica. In addition, cultured bovine pulmonary epithelial (BPE) cells were stimulated with heat-killed and live M. haemolytica bacteria, lipopolysaccharide (LPS), lipoteichoic acid (LTA), interleukin-1 (IL-1), and zymosan activated plasma (ZAP) to determine whether they might release extracellular ATP during in vitro infection. Calves experimentally exposed to M. haemolytica had an approximately 2-fold higher level of ATP in their BAL samples compared to control. BPE cells exposed to increasing numbers of heat-killed or live M. haemolytica had significantly increased levels of ATP release as compared to time-matched controls. Finally, BPE cells treated with several concentrations of LPS and IL-1 had increases in ATP release as compared to time-matched controls. This increase appeared to be a result of active ATP secretion by the cells, as cell viability was similar between treated and non-treated cells. Neither ZAP nor LTA induced any ATP release by the cells. In conclusion, ATP levels are elevated in lung secretions from calves infected with M. haemolytica. In addition, lung epithelial cells can actively release ATP when exposed to heat-killed or live M. haemolytica, LPS or IL-1. 相似文献
27.
A review and meta‐analysis of growth and life‐history traits of a declining European freshwater fish,crucian carp Carassius carassius 下载免费PDF全文
Ali Serhan Tarkan David Almeida Michael J. Godard Özcan Gaygusuz Mark Rylands Carl D. Sayer Grzegorz Zięba Gordon H. Copp 《水产资源保护:海洋与淡水生态系统》2016,26(1):212-224
28.
William A. Battaglin Mark W. Sandstrom Kathryn M. Kuivila Dana W. Kolpin Michael T. Meyer 《Water, air, and soil pollution》2011,218(1-4):307-322
Fungicides are used to prevent foliar diseases on a wide range of vegetable, field, fruit, and ornamental crops. They are generally more effective as protective rather than curative treatments, and hence tend to be applied before infections take place. Less than 1% of US soybeans were treated with a fungicide in 2002 but by 2006, 4% were treated. Like other pesticides, fungicides can move-off of fields after application and subsequently contaminate surface water, groundwater, and associated sediments. Due to the constant pressure from fungal diseases such as the recent Asian soybean rust outbreak, and the always-present desire to increase crop yields, there is the potential for a significant increase in the amount of fungicides used on US farms. Increased fungicide use could lead to increased environmental concentrations of these compounds. This study documents the occurrence of fungicides in select US streams soon after the first documentation of soybean rust in the US and prior to the corresponding increase in fungicide use to treat this problem. Water samples were collected from 29 streams in 13 states in 2005 and/or 2006, and analyzed for 12 target fungicides. Nine of the 12 fungicides were detected in at least one stream sample and at least one fungicide was detected in 20 of 29 streams. At least one fungicide was detected in 56% of the 103 samples, as many as five fungicides were detected in an individual sample, and mixtures of fungicides were common. Azoxystrobin was detected most frequently (45% of 103 samples) followed by metalaxyl (27%), propiconazole (17%), myclobutanil (9%), and tebuconazole (6%). Fungicide detections ranged from 0.002 to 1.15 ??g/L. There was indication of a seasonal pattern to fungicide occurrence, with detections more common and concentrations higher in late summer and early fall than in spring. At a few sites, fungicides were detected in all samples collected suggesting the potential for season-long occurrence in some streams. Fungicide occurrence appears to be related to fungicide use in the associated drainage basins; however, current use information is generally lacking and more detailed occurrence data are needed to accurately quantify such a relation. Maximum concentrations of fungicides were typically one or more orders of magnitude less than current toxicity estimates for freshwater aquatic organisms or humans; however, gaps in current toxicological understandings of the effects of fungicides in the environment limit these interpretations. 相似文献
29.
Energy and resource consumption of land-based Atlantic salmon smolt hatcheries in the Pacific Northwest (USA) 总被引:1,自引:0,他引:1
John Colt Steve Summerfelt Tim Pfeiffer Sveinung Fivelstad Michael Rust 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):94-108
This paper evaluates the resource and energy requirements of six different types of land-based, hatchery production systems located in the U.S. Pacific Northwest: flow-through with a gravity water supply, flow-through with a pumped water supply, flow-through with pure oxygen, partial reuse system, partial reuse with heating, and a reuse system for the production of Atlantic salmon (Salmo salar) smolts. Key parameters used in the evaluation include direct energy, indirect energy, transportation energy, greenhouse gas emissions, and pollutant discharges.Power (electricity and natural gas) and feed energy accounted for the majority of the required energy for all the rearing option evaluated. The sum of the fixed capital and chemicals components accounted for less than 2–12% of the total energy budget for any rearing option. The energy efficiency (energy output/energy input) of the six options ranges from 0.97% for flow-through with pumped supply to 3.49% for the flow-through with gravity supply. The rearing options with the three highest energy efficiencies were flow-through with gravity supply (3.49%), partial reuse (2.75%), and reuse (2.64%).On a kg of smolt produced basis, the six rearing options showed a wide range in performance. The reuse system had the lowest water (2 m3 kg− 1) and land (0.13 m2 kg− 1) requirements and the third lowest total energy requirement (288 MJ kg− 1). The partial reuse system had the second lowest total power requirement (276 MJ kg− 1), a low land requirement (0.21 m2 kg− 1), and moderate water requirements (33 m3 kg− 1). The partial reuse with temperature control had the second highest total power requirement (657 MJ kg− 1) and land and water requirements similar to the partial reuse system without temperature control. The flow-through system with pumped water supply had the highest water (289 m3 kg− 1), land (2.19 m2 kg− 1), and energy requirements (786 MJ kg− 1) of any of the rearing options. By comparison, the flow-through system with gravity water supply had the lowest energy requirement (218 MJ kg− 1), a moderate land requirement (0.78 m2 kg− 1), and a high water requirement (214 m3 kg− 1). The ranking of the six rearing options based capital and operating costs are likely to be quite different from those based on energy, water, and greenhouse gas emissions. 相似文献
30.