Studies were carried out to determine the fate of virulent Toxoplasma gondii challenge in immune animals. Toxoplasma strains isolated from human, swine, rabbit and cat hosts were used for the primary immunization of mice. Brains were removed at various intervals after the challenge and subinoculated into normal mice. Prior immunization with one of the nine toxoplasma strains enabled the mice either to eliminate or harbour the challenge organisms without loss of virulence. The outcome of challenge infection was dependent on the parasite strain used for the immunization and the time interval between challenge and subinoculation. Mice immunized with strain KSU isolated from a cat eliminated repeatedly administered challenge from 80% of animals. Strain S 162 isolated from a swine eliminated similar challenge from only 20% of animals. The results indicate that the extent of protection against virulent T. gondii can vary widely and is related to the immunogenicity of the original immunizing strain. 相似文献
Precision irrigation utilizing soil moisture data and valve-in head sprinkler systems may be a viable solution for sustainable water management on complex turfgrass areas. There is currently no research investigating the factors that influence soil moisture and turfgrass quality variability within sand-capped golf course fairways to aid in precision irrigation-related management decisions. Therefore, the objective of this study was to measure several turfgrass and soil characteristics from two sand-capped fairways during dry down events from either rainfall or irrigation to determine their relationship and contribution to soil moisture and turfgrass quality variability. Considerable spatiotemporal variability was observed within the two fairways during the dry down periods. Factors that were found to have a significant influence on soil moisture and turfgrass quality were sand capping depth, elevation, and thatch depth, but these relationships were not consistent between rainfall versus irrigation events, days after dry down, or even the specific fairways. Also, the direction of many of the relationships were opposite of what was expected. These findings highlight the complexity of soil moisture and turfgrass quality variability on sand-capped golf course fairways. To incorporate soil moisture sensor technologies into large-scale precision irrigation practices, mapping soil moisture with an understanding of contributing factors is a necessary preliminary step. Although there are several current practical limitations, the information presented in this study provides a foundation for future research.
Backcross breeding programs have been used to transfer disease resistance and other traits from one forest tree species to another in order to meet restoration objectives. Evaluating the field performance of such material is critical for determining the success of breeding programs. In eastern North America, The American Chestnut Foundation has a backcross breeding program that uses Chinese chestnut (Castanea mollissima Blume) to introduce resistance of the fungal pathogen chestnut blight [Cryphonectria parasitica (Murr.) Barr.] to the native American chestnut [Castanea dentata (Marsh.) Borkh.]. We compared physiological and morphological characteristics among seedlings of American chestnut, Chinese chestnut, and BC1F3, BC2F3, and BC3F3 hybrid chestnuts during their fourth growing season after field-planting. American chestnut and the BC3F3 breeding generation displayed photosynthetic light-response curves that were similar to each other but different from Chinese chestnut. Rates of photosynthesis were higher for American chestnut and the BC3F3 breeding generation when compared to Chinese chestnut for light levels ≥800 μmol m?2 s?1 photosynthetic photon flux density and for maximum photosynthetic capacity. Leaf morphology variables were not different between American chestnut and any of the breeding generations, but leaf area (on a per leaf basis) of Chinese chestnut was lower than that of any other chestnut type. Our results suggest that backcross breeding can be used to transfer desirable traits for restoration of native species threatened by non-native pathogens. 相似文献
Globally, aquaculture production is faced with numerous challenges, notable among which is water quality. The study determined seasonal environmental changes in Lake Volta and its implications on cage production of Nile tilapia, Oreochromis niloticus in experimental and commercial farm (Sun Woo Farm) set-ups. For each set-up, three cages were used, each measuring 81 and 162.5 m3 for experimental and commercial, respectively. The cages were stocked with 22.5 g fingerlings at a rate of 40 and 80 m?3 in experimental and commercial cages, respectively, and fed commercial diet. The growth performance, yield, economics, and selected water quality parameters were monitored during the dry and wet seasons between November 2014 and July 2015. There were no differences in water quality parameters between dry and wet seasons (p?>?0.05). Water quality for both seasons remained within limits required for good Tilapia growth in both set-ups. There were minimal variations in growth characteristics between seasons which were not significant; however, final mean weight and yields were high in the dry seasons in both set-ups. The cost of feed and fingerling accounting for about 80% of total costs were the major components identified to affect cost of production. A higher profit index and returns on investment were observed in the dry season in both set-ups and was linked to higher survival rates in the season. Production of Nile tilapia in cages can be cultured throughout the year without any adverse effects as exhibited in similar production patterns in both experimental and commercial set-ups. 相似文献
Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle. 相似文献
Gross and light microscopic features of transitional cell carcinomas (TCC) of the urinary tract were examined in Beagle dogs used for the study of the long-term effects of low-dose, whole-body, 60Co gamma radiation. Thirty-eight cases of TCC occurred among 990 dogs that were from 0 to 14 years of age. There was no conclusive evidence of a radiation effect. The 38 TCC were equally divided between male and female dogs, but there was a significant difference in the sex distribution of urethra-origin TCC. Eleven males had a primary urethral TCC compared to only two females. There was no significant difference between the urethra-origin and bladder-origin TCCs in the number of tumors that caused clinical signs, metastasized, or that contributed to the death of the dog. All cases of urethral TCC in male dogs occurred in the prostatic urethra. The majority of these cases were not recognized to be neoplasms at gross necropsy, but microscopic examination revealed the TCC. Our findings differ from previous reports stating that TCC occurs more frequently in female than male dogs, and they especially differ from reports claiming that urethra-origin TCC is predominantly a disease of female dogs. 相似文献