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81.
Haoming Wu Qui D. Nguyen Tu T. M. Tran Minh T. Tang Takeshi Tsuruta Naoki Nishino 《Animal Science Journal》2019,90(3):445-452
Microbiota of the gut, milk, and cowshed environment were examined at two dairy farms managed by automatic milking systems (AMS). Feed, rumen fluid, feces, milk, bedding, water, and airborne dust were collected and the microbiota on each was assessed by Illumina MiSeq sequencing. The most abundant taxa in feed, rumen fluid, feces, bedding, and water were Lactobacillaceae, Prevotellaceae, Ruminococcaceae, Ruminococcaceae, and Lactobacillaceae, respectively, at both farms. Aerococcaceae was the most abundant taxon in milk and airborne dust microbiota at farm 1, and Staphylococcaceae and Lactobacillaceae were the most abundant taxa in milk and airborne dust microbiota at farm 2. The three most prevalent taxa (Aerococcaceae, Staphylococcaceae, and Ruminococcaceae at farm 1 and Staphylococcaceae, Lactobacillaceae, and Ruminococcaceae at farm 2) were shared between milk and airborne dust microbiota. Indeed, SourceTracker indicated that milk microbiota was related with airborne dust microbiota. Meanwhile, hierarchical clustering and canonical analysis of principal coordinates demonstrated that the milk microbiota was associated with the bedding microbiota but clearly separated from feed, rumen fluid, feces, and water microbiota. Although our findings were derived from only two case studies, the importance of cowshed management for milk quality control and mastitis prevention was emphasized at farms managed by AMS. 相似文献
82.
Fermentation and microbial population dynamics during the ensiling of native grass and subsequent exposure to air
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To study the microbial population and fermentation dynamics of large needlegrass (LN) and Chinese leymus (CL) during ensiling and subsequent exposure to air, silages were sampled and analyzed using culture‐based techniques and denaturing gradient gel electrophoresis (DGGE). A total of 112 lactic acid bacteria (LAB) strains were isolated and identified using the 16S rRNA sequencing method. Lactic acid was not detected in the first 20 days in LN silage and the pH decreased to 6.13 after 45 days of ensiling. The temperature of the LN silage increased after approximately 30 h of air exposure and the CL silage showed a slight temperature variation. Enterococcus spp. were mainly present in LN silage. The proportion of Lactobacillus brevis in CL silage increased after exposure to air. LN silage with a higher proportion of Enterococcus spp. and propionic acid concentration did not show higher fermentation quality or aerobic stability than CL silage, which had a higher concentration of acetic acid, butyric acid and increased proportion of L. brevis after exposure to air. 相似文献
83.
Atsuo IIDA Hitoshi TAKEMAE Ronald TARIGAN Ryosuke KOBAYASHI Hirokazu KATO Hiroshi SHIMODA Tsutomu OMATSU Supratikno Chaerul BASRI Ni Luh Putu Ika MAYASARI Srihadi AGUNGPRIYONO Ken MAEDA Tetsuya MIZUTANI Eiichi HONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1068
Here, we performed next-generation sequencing (NGS) on six large flying foxes (Pteropus vampyrus) collected in Indonesia. Seventy-five virus species in the liver tissue of each specimen were listed. Viral homologous sequences in the bat genome were identified from the listed viruses. This finding provides collateral evidence of viral endogenization into the host genome. We found that two of the six specimens bore partial sequences that were homologous to the plant pathogens Geminiviridae and Luteoviridae. These sequences were absent in the P. vampyrus chromosomal sequences. Hence, plant viral homologous sequences were localized to the hepatocytes as extrachromosomal DNA fragments. Therefore, this suggests that the bat is a potential carrier or vector of plant viruses. The present investigation on wild animals offered novel perspectives on viral invasion, variation, and host interaction. 相似文献
84.
85.
Nishino Y Funaba M Fukushima R Mizutani T Kimura T Iizuka R Hirami H Hara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(10):1167-1170
Borna disease virus (BDV) infection has been suggested to cause spontaneous neurological disease in cats referred to as staggering disease. However the evaluation of BDV infection in neurologically asymptomatic cats remained unclear. In the present study, BDV infected, asymptomatic cats in Tokyo were surveyed both by the presence of plasma antibodies against BDV-p24 and -p40 and by RNA detection in peripheral blood mononuclear cells. Seven of 32 domestic cats (21.9%) were serologically or genetically judged to be BDV-infected. Six cats were positive for anti-BDV antibody and two cats were positive for BDV RNA. Within the 2 RNA-positive cats, only one was positive for anti-BDV antibodies. Furthermore, the findings of anti-BDV-p40 and anti-BDV-p24 antibody-positive cats did not completely overlap. These results suggest that there are neurologically asymptomatic domestic cats infected with BDV present in the Tokyo area. 相似文献
86.
Yoshimi?NiwanoEmail author Fumiaki?Beppu Taichi?Shimada Rika?Kyan Kazumasa?Yasura Minori?Tamaki Michinori?Nishino Yoshiyuki?Midorikawa Hiroki?Hamada 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(1):6-10
To search for plant foodstuffs with potent anti-obese activity, we conducted a large scale screening based on the inhibitory
activity on adipogenesis and the facilitating activity on adipolysis in vitro. That is, inhibition of intracellular lipid accumulation and facilitation of lipid degradation in 3T3-L1 adipocytes were
extensively screened from ethanol and hexane extracts of approximately 100 kinds of plant foodstuffs marketed in Okinawa prefecture,
which has been famous for the highest prevalence of exceptionally long-lived individuals in the world. Among them thirty one
foodstuffs showed potent inhibitory activity on intracellular lipid accumulation in 3T3-L1 adipocytes, whereas only four foodstuffs
showed clear facilitating effect on lipid degradation in 3T3-L1 adipocytes. Although further study to examine the in vivo effects on adipogenesis and adipolysis is required, this is the first study to investigate anti-obese characteristics of
wide range of traditional Okinawa foodstuffs so that the results give useful information to take another look at Okinawa food
culture. 相似文献
87.
Yoshihiko Nishino Gérard Janin Yuji Yainada Daisuke Kitano 《Journal of Wood Science》2000,46(4):267-272
Color measurements were made for sapwood specimens of 26 hardwood species. The densities of the specimens ranged from 258.5 to 945.6kg/m3. The colorimetric values and densities were compared among the species. The tristimulus values ofX, Y, andZ were poorly correlated with the densities. The chromaticity coordinatex had a good linear correlation with the densities of the wood specimens, especially in the transverse planes. The transverse plane of wood fibers is thought to emphasize the relative values in the long wavelength region of the visible ray.Part of this report was presented at the 49th annual meeting of the Japan Wood Research Society, Tokyo, April 1999 相似文献
88.
Anemia with chronic renal disorder and disrupted metabolism of erythropoietin in ICR-derived glomerulonephritis (ICGN) mice 总被引:1,自引:0,他引:1
Yamaguchi-Yamada M Manabe N Uchio-Yamada K Akashi N Goto Y Miyamoto Y Nagao M Yamamoto Y Ogura A Miyamoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):423-431
The ICR-derived glomerulonephritis (ICGN) mouse, a new inbred mouse strain with a hereditary nephrotic syndrome, is considered to be a good model of human idiopathic nephrotic syndrome and notably exhibits proteinuria and hypoproteinemia from the neonatal stage. In chronic renal disorder (CRD), anemia is a major subsequent symptom (renal anemia). The precise cause of renal anemia remains unclear, primarily owing to the lack of appropriate spontaneous animal models for CRD. To establish adequate animal models for anemia with CRD, we examined the hematological-biochemical properties and histopathological characteristics. With the deterioration of renal function, ICGN mice developed a normochromic and normocytic anemia, and exhibited normochromic and microcytic at the terminal stage. The expression of erythropoietin (EPO) mRNA both in the kidneys and liver and the EPO leak into the urine were observed in ICGN mice, indicating a disrupted metabolism of EPO in ICGN mice. In addition, a lack of iron induced by the hemolysis in the spleen and the leak of transferrin into urine as proteinuria aggravated the anemic condition. In conclusion, the ICGN mouse is a good model for anemia with CRD. 相似文献
89.
90.
Sekizaki T Nishiya H Nakajima S Nishizono M Kawano M Okura M Takamatsu D Nishino H Ishiji T Osawa R 《Avian diseases》2008,52(1):183-186
Streptococcus gallolyticus subsp. gallolyticus causes endocarditis in humans and acute septicemia in domestic birds. We describe here the infective endocarditis caused by the bacterium found among clinically healthy broilers at two abattoirs in Japan. The chickens were thought to be healthy because of the lack of clinical symptoms and normal levels of mortality before slaughtering. At the time of inspection, some chickens were condemned because of organ disorders characterized by vegetative valvular endocarditis as well as focal necrosis in heart, liver, and spleen. Streptococcus gallolyticus subsp. gallolyticus was isolated from the organs as a pure culture, indicating that the bacterium probably was the causative agent of the disorders. Amplified fragment length polymorphism analysis of the isolates collected at the abattoirs from chickens grown in nine different farms indicated that the isolates were different variants of the same clonal lineage and may have been derived from the same ancestor. These results suggest that S. gallolyticus subsp. gallolyticus causes infectious endocarditis in chickens and that healthy chickens may possess the bacterium in their normal flora as an opportunistic pathogen. 相似文献