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81.
Antonina Klimenko Robin Huber Laurence Marcourt Estelle Chardonnens Alexey Koval Yuri S. Khotimchenko Emerson Ferreira Queiroz Jean-Luc Wolfender Vladimir L. Katanaev 《Marine drugs》2021,19(1)
Triple-negative breast cancer (TNBC) represents the deadliest form of gynecological tumors currently lacking targeted therapies. The ethanol extract of the North Pacific brittle star Ophiura sarsii presented promising anti-TNBC activities. After elimination of the inert material, the active extract was submitted to a bioguided isolation approach using high-resolution semipreparative HPLC-UV, resulting in one-step isolation of an unusual porphyrin derivative possessing strong cytotoxic activity. HRMS and 2D NMR resulted in the structure elucidation of the compound as (3S,4S)-14-Ethyl-9-(hydroxymethyl)-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoic acid. Never identified before in Ophiuroidea, porphyrins have found broad applications as photosensitizers in the anticancer photodynamic therapy. The simple isolation of a cytotoxic porphyrin from an abundant brittle star species we describe here may pave the way for novel natural-based developments of targeted anti-cancer therapies. 相似文献
82.
Tatiana I. Terpinskaya Alexey V. Osipov Elena V. Kryukova Denis S. Kudryavtsev Nina V. Kopylova Tatsiana L. Yanchanka Alena F. Palukoshka Elena A. Gondarenko Maxim N. Zhmak Victor I. Tsetlin Yuri N. Utkin 《Marine drugs》2021,19(2)
Among the brain tumors, glioma is the most common. In general, different biochemical mechanisms, involving nicotinic acetylcholine receptors (nAChRs) and the arachidonic acid cascade are involved in oncogenesis. Although the engagement of the latter in survival and proliferation of rat C6 glioma has been shown, there are practically no data about the presence and the role of nAChRs in C6 cells. In this work we studied the effects of nAChR antagonists, marine snail α-conotoxins and snake α-cobratoxin, on the survival and proliferation of C6 glioma cells. The effects of the lipoxygenase and cyclooxygenase inhibitors either alone or together with α-conotoxins and α-cobratoxin were studied in parallel. It was found that α-conotoxins and α-cobratoxin promoted the proliferation of C6 glioma cells, while nicotine had practically no effect at concentrations below 1 µL/mL. Nordihydroguaiaretic acid, a nonspecific lipoxygenase inhibitor, and baicalein, a 12-lipoxygenase inhibitor, exerted antiproliferative and cytotoxic effects on C6 cells. nAChR inhibitors weaken this effect after 24 h cultivation but produced no effects at longer times. Quantitative real-time polymerase chain reaction showed that mRNA for α4, α7, β2 and β4 subunits of nAChR were expressed in C6 glioma cells. This is the first indication for involvement of nAChRs in mechanisms of glioma cell proliferation. 相似文献
83.
Yuri W. L. DE SOUSA Erika BUSCARDO Carlos A. N. QUESADA Henrique E. M. NASCIMENTO Laszlo NAGY 《土壤圈》2021,31(6):964-972
In non-flooded lowland rain forests with low soil phosphorus (P) in parts of Amazonia, P cycling largely occurs via leaf litter recycling by arbuscular mycorrhizal (AM) fungal symbionts. Occasional high input of P into these ecosystems occurs during drought years with increased litterfall. As the length and frequency of drought events are projected to increase in the region, a single-dose nutrient addition experiment was carried out to test how this would impact P cycling. An application rate of 4 kg P ha-1 was used, which corresponds to twice the amount of litter-derived P in an average year. It was hypothesized that i) the added mineral P would be immobilized by soil microorganisms, leading to measurable increase in soil microbial biomass carbon (C) and P and ii) AM colonization rate would be reduced by the pulse in mineral P available for plant uptake. The results did not support either of our hypotheses. The addition of P did not have an effect on AM root colonization, nor was P immobilized by soil microbiota during the experimental period. The lack of a difference between the control and treatment at our study site could be attributed to the relatively low one-off dose of P applied that did not change either the colonization rate of roots by AM fungi or the amount of soil available labile P. To obtain a mechanistic understanding of the availability, capture, and use of P by plant-symbiont associations in tropical rain forest ecosystems, further integrated studies of the soil-plant system combining long-term nutrient manipulations, modeling, and experimental approaches are required. 相似文献
84.
Koji Misumi Yuri Hirayama Misae Suzuki Michiko Nakai Junko Noguchi Hiroyuki Kaneko Kazuhiro Kikuchi 《Animal Science Journal》2014,85(2):112-117
Collection efficacy and in vitro embryo developmental ability of oocytes obtained from Duroc‐breed ovary donors at different stages of the estrous cycle (days 6, 12 and 16 after estrus) were performed. The numbers of collected oocytes did not differ significantly among the different estrous cycle groups (total 72–90 oocytes per gilt). However, the blastocyst rates of oocytes collected on days 12 and 16 (9.2% and 19.4%, respectively) were significantly higher than those on day 6 (1.1%). More oocytes were obtained on day 16 from small follicles (<2 mm in diameter; 85.3 oocytes per gilt) than from medium‐sized (≥2–<6 mm) and large (≥6 mm) follicles (17.5 and 12.8 oocytes, respectively). The blastocyst rates in both the medium‐sized and large follicle groups (20.0% and 19.2%, respectively) were significantly higher than that in the small follicle group (6.3%). The blastocyst cell numbers in both the medium‐sized and large follicle groups (39.4 and 43.3 cells, respectively) were significantly higher than that in the small follicle group (30.6 cells). The results suggest that oocyte collection from cycling Duroc pigs can be carried out efficiently from the late luteal to follicular stage. Those oocytes collected from medium‐sized and large follicles show better embryo development. 相似文献
85.
Katsuhiko Yoshizawa Yuko Emoto Yuichi Kinoshita Ayako Kimura Norihisa Uehara Takashi Yuri Nobuaki Shikata Airo Tsubura 《Journal of toxicologic pathology》2013,26(1):61-66
Uterine deciduomas were found in two female virgin rats, a 15-week-old Lewis rat and a 7-week-old Sprague-Dawley rat. The firm white nodules were located at the base of unilateral uterine horns and were approximately 6 mm and 4 mm in diameter. Histopathologically, the nodules were composed of three areas, each with a distinct type of proliferating cells: large epithelioid decidual cells with round nuclei, prominent nucleoli and abundant eosinophilic cytoplasm (antimesometrial region); compact spindle-shaped cells with oval nuclei and vacuolar cytoplasm (transitional region); and pleomorphic and spiny cells with round to oval nuclei and compact eosinophilic cytoplasm (mesometrial region). These cells proliferated in sheet-like arrangements and transformed into the other types of cells located in surrounding regions. Immunohistochemically, proliferating cells in all regions were strongly positive for proliferating cell nuclear antigen. The proliferating cells were positive for vimentin, and large decidual cells were positive for common acute lymphoblastic leukemia antigen 10, a marker of uterine interstitial cells. Large decidual cells were positive for α-smooth muscle actin and desmin, suggesting differentiation into muscular cells. Progesterone receptor was expressed in all cell types; however, estrogen receptor α was not expressed in the antimesometrial region. These extremely rare tumor-like nodules represent nonneoplastic lesions referred as decidual reactions of endometrial interstitial cells, and their biological behavior is that of a space-occupying benign tumor in young rats. Our cases might provide information as a historical control in toxicity and pharmacological studies in rats. 相似文献
86.
Jun Murase Yuri Matsui Atsuko Sugimoto Makoto Kimura 《Soil biology & biochemistry》2006,38(12):3483-3491
Rice straw is a major organic material applied to rice fields. The microorganisms growing on rice-straw-derived carbon have not been well studied. Here, we applied 13C-labeled rice straw to submerged rice soil microcosms and analyzed phospholipid fatty acids (PLFAs) in the soil and percolating water to trace the assimilation of rice-straw-derived carbon into microorganisms. PLFAs in the soil and water were markedly enriched with 13C during the first 3 days of incubation, which indicated immediate incorporation of rice-straw-derived carbon into microbial biomass. The enrichment of PLFAs in the percolating water with 13C suggested that microorganisms other than the population colonizing rice straw also assimilated rice-straw-derived carbon or that some bacterial groups were selectively released from the straw. The microbial populations could be categorized into two communities based on the carbon isotope data of the PLFAs: those derived from rice straw and those derived from soil organic matter (SOM). The composition of the PLFAs from the two communities differed, which indicated the assimilation of rice-straw-derived carbon by a subset of microbial populations. The composition of rice-straw-derived PLFAs in the percolating water was also distinct from that in the soil. 相似文献
87.
Alba L. A. SKORUPA Sérgio H. G. SILVA Giovana C. POGGERE Diego TASSINARI Leandro C. PINTO Yuri L. ZINN Nilton CURI 《土壤圈》2017,27(4):747-757
Soils result from the interaction of five independent formation factors.If one factor varies,while the others remain constant,different soils can be produced.Herein,we demonstrated an opposing trend,wherein two soils were similar,despite considerable differences in all factors of soil formation.We sampled two Inceptisols (Oxic Dystrudepts) formed on different parent materials (gneiss vs.mica schist),climate (tropical altimontane vs.warmer,drier plateau),topography (1 650 m,45% slope vs.1000 m,8% slope),time (rejuvenated vs.old,stable surface),and vegetation (rainforest vs.Cerrado savanna).The two soils had similar chemical properties,whereas the soil on mica schist had finer particle size distribution,lower porosity,and lower saturated hydraulic conductivity.These properties were related to a coarser blocky microstructure compared to the soil on gneiss.Both soils presented active mineral weathering and pronounced pedoplasmation,demonstrated by clay contents > 300 g kg-1,although only the Dystrudept on gneiss possessed coarse rock fragments.The C horizons of both soils presented fragmented clay coatings suggestive of argilluviation,likely relict,because they were not observed in the B horizons.The similarities in many properties of the two Dystrudepts,despite contrasting factors of soil formation,suggest converging evolution and that soil classification at the subgroup level was efficient in grouping similar formative processes in tropical conditions.Moreover,this work revealed that similar pedogenic processes acting on different factors of soil formation can result in similar soil properties,at least for Inceptisols where further soil development is hindered by topographic limitations. 相似文献
88.
Youichi Ikeshita Yuri Kanamori Nobuyuki Fukuoka Jun Matsumoto Yasutaka Kano 《Scientia Horticulturae》2010
To investigate the effects of night-time temperature on cell and fruit size, and sugar accumulation in watermelon fruit, fruits were treated with high night-time temperatures in a greenhouse. The minimum night-time ambient temperature of the heating box (18 °C) was approximately 6 °C higher than that of the control. The length, diameter and weight of heat-treated fruit at the end of heating treatment, 16 days after anthesis (DAA), were significantly greater than that of control fruit, but those at harvesting, 42 DAA, were almost the same in both treatments. Mean cell size of the outer portion of heat-treated fruit at 16 DAA was significantly larger than that of the control. Cell size of the fruits at 42 DAA did not differ between heat-treated and control fruits. Sucrose, glucose and fructose content of fruit at 16 DAA did not differ between heat-treated and control fruit. However, sucrose content of the outer portion of heat-treated fruit was 162% of that of control fruit at 42 DAA. Glucose and fructose contents at 42 DAA did not differ between heat-treated and control fruit, except glucose content of outer portion. 相似文献
89.
Doi T Kotani Y Takahashi K Hashimoto S Yamada N Kokoshima H Tomonari Y Wako Y Tsuchitani M 《Journal of toxicologic pathology》2010,23(2):103-106
Spontaneous malignant mesothelioma was found in a 104-week-old male Crj:CD(SD) rat. The tumor was scattered on the surface of the lung, heart, mediastinal pleura and thoracic wall and metastasized to the alveolar septa. Histopathologically, small flattened or cuboidal tumor cells proliferated with stroma, formed almost normal papillary structures and reacted positively to colloidal iron stain and immunohistochemical staining for mesothelin. Round hyalinous stromata were pronounced, which is a characteristic feature, and the possible reason for this is as follows; at first, a small amount of collagen fibers was formed in the center of the clusters of several tumor cells, and then the cell clusters expanded like balloons with an increase in the collagen fibers. 相似文献
90.
Rubtsov YP Niec RE Josefowicz S Li L Darce J Mathis D Benoist C Rudensky AY 《Science (New York, N.Y.)》2010,329(5999):1667-1671
Tissue maintenance and homeostasis can be achieved through the replacement of dying cells by differentiating precursors or self-renewal of terminally differentiated cells or relies heavily on cellular longevity in poorly regenerating tissues. Regulatory T cells (T(reg) cells) represent an actively dividing cell population with critical function in suppression of lethal immune-mediated inflammation. The plasticity of T(reg) cells has been actively debated because it could factor importantly in protective immunity or autoimmunity. By using inducible labeling and tracking of T(reg) cell fate in vivo, or transfers of highly purified T(reg) cells, we have demonstrated notable stability of this cell population under physiologic and inflammatory conditions. Our results suggest that self-renewal of mature T(reg) cells serves as a major mechanism of maintenance of the T(reg) cell lineage in adult mice. 相似文献