Effects of isoflurane on somatosensory-evoked potentials in calves: A pilot study

Somatosensory evoked potentials (SSEP) are used to monitor sensory function and are often recorded under general anesthesia. The objective of the study was to evaluate the effects of isoflurane on SSEPs in calves as it has not been reported. Eight calves (mean age: 40 days), were included in the study. Calves were anesthetized with a randomized sequence of four different isoflurane partial pressures. Blood gas analysis was performed before each measurement. SSEP were induced by repeated stimulation of the common dorsal digital nerve III. SSEPs were recorded from the lumbo-sacral junction (s-SSEP) and the head (c-SSEP). Latency and inter-amplitude of each peak were measured. For s-SSEP: One negative (N_sp1) and two positive (P_sp1 and P_sp2) peaks were identified in all tracings except for two calves. There was a significant effect of isoflurane on the latency of P_sp2 (P = 0.01). Inter-amplitude decreased significantly with PaO₂, PaCO₂ and temperature (P < 0.05). P_sp2 latency decreased with PaO₂ (P = 0.01). For c-SSEP: two positive (P_c1 and P_c2) and two negative (N_c1 and N_c2) peaks were identified. There were identifiable peaks for the analysis of P_c1 latencies only. There was a significant positive linear relation between end-tidal isoflurane partial pressure (ET_iso) and P_c1 latency (P = 0.04). None of the co-variables had a significant effect on the latency of P_c1 (P > 0.1). Isoflurane has a major impact on the recording of c-SSEP. Recording should be done at the lowest ET_iso as possible, and anesthesia parameters should be kept constant.     

Conservation education needs more parasites

Formal training in conservation biology education is an important component of the suite of solutions to current environmental problems. As conservation biology textbooks underpin many conservation education programs at both undergraduate and graduate levels, the portrayal of conservation issues and coverage of specific biotic groups plays a potentially important role in guiding the training of the next generation of conservation professionals. Parasites represent the majority of species diversity on earth, play critical roles in ecology and evolution, and are often at higher risk of decline and extinction than their free-living counterparts. Yet parasites continue to receive scant attention from conservation scientists and educators. We searched the index of 77 English language conservation biology textbooks for parasite or pathogen related content, published between 1970 and 2009. When present, we categorized a textbook’s parasite content as positive, neutral or negative with respect to the author’s portrayal of parasite biodiversity. The vast majority (72%) of conservation textbooks either portray parasites uniquely as threats to conservation goals established for free-living species or do not mention parasites at all. While no single textbook can be expected to provide extensive treatment of conservation-related topics across all biological groups, we outline three reasons why parasites should feature more prominently in formal conservation education, and suggest several ways in which greater incorporation of these parasite topics could strengthen the teaching and practice of conservation biology.     

Effect of Initial Spacing on First Thinning Product Recovery,and Financial Outcomes in Whitewood (<Emphasis Type="Italic">Endospermum medullosum</Emphasis>) Plantations in Vanuatu

This paper investigates the products from first thinning at age 6 in Endospermum medullosum (whitewood) plantations in Vanuatu at three stocking densities, and the financial outcomes from several silvicultural thinning regimes based on these spacings. Whitewood plantings established at stockings rates of 417, 625 and 833 stems per hectare were thinned at age 6 to approximately 45 % retained basal area. The thinning products were measured during harvest and value was estimated based on costs of production and appropriate margins relative to radiata pine products already on the market. Financial modelling for the whole rotation was undertaken for each of the spacing and thinning scenarios in the trial. The volume and size of poles and posts at first thinning was related to spacing. Low stocking density produced smaller quantities of larger poles, whereas higher stocking densities produced larger quantities of smaller posts. However, the monetary value of larger products grown under lower stocking rates was less than the value of the greater volume of product at higher stocking rates. This greater volume at higher stockings resulted in higher NPV for regimes with higher initial stockings for modelled scenarios regardless of harvest age. An exception was a scenario where no market for thinnings is available. A direct silvicultural regime (i.e. one with low planting density and no thinnings) had the highest NPV due to the higher price per cubic metre of larger diameter logs, achieved more rapidly because thinning did not remove basal area. Where thinning products are marketable, higher initial planting densities provide higher returns. Where access to markets for thinning products is difficult or where infrastructure is expected to improve in the future, planting at low stocking and without any thinning provides a high return on investment.     

Social and ecological issues for private native forestry in north-eastern New South Wales,Australia

Forests in north-eastern New South Wales have often been the focus of controversy. The tension between production and preservation continues and hampers current negotiations for a code of practice for private native forestry. The structure of many private forests reflects past mismanagement, and silvicultural intervention would benefit both conservation and production objectives, but such intervention is rarely financially viable. This paper sets out the economic and ecological basis for private native forestry. Both the timber industry and nature-based tourism are major contributors to the local economy, and both rely in part on private native forests. Draft regulations currently under negotiation do not offer incentives for improved private forest management.

Fatal mycotic dermatitis in captive brown tree snakes (Boiga irregularis).

Cutaneous fungal infections occurred in four captive brown tree snakes (Boiga irregularis). The ventral scales were most commonly affected, and lesions began as areas of erythema and edema with vesicle formation, followed by development of caseous brown plaques. Lesions usually started where ventral scales overlapped and spread rapidly. All snakes died within 14 days after clinical signs were first noted. The deaths of three of the snakes were directly attributable to the cutaneous disease; the other snake died from renal failure and visceral gout, most likely induced by gentamicin therapy. Histologically, lesions consisted of epidermal hyperplasia and hyperkeratosis, with foci of epidermal necrosis, intraepidermal vesicle formation, and subacute inflammation of the underlying dermis. These lesions were associated with bacteria and numerous septate, branched fungal hyphae within the epidermis and overlying serocelluar crusts. Hyphae that penetrated through the superficial surface of the epidermis often formed terminal arthroconidia. The same species of fungus was isolated in pure culture from the skin of three snakes, but fungal cultures were not performed on samples from the fourth snake. The fungus has been identified as the Chrysosporium anamorph of Nannizziopsis vriesii based on its formation of solitary dermatophytelike aleurioconidia and alternate and fission arthroconidia. The source of the fungus in this outbreak was not determined; however, the warm, moist conditions under which the snakes were housed likely predisposed them to opportunistic cutaneous fungal infections.     

Ethanol Fermentation of Starch from Field Peas

Field peas (Pisum sativum) were evaluated as a potential feedstock for ethanol production. Ground peas were dry‐milled and separated into starch, protein, and fibrous fractions by air classification. Starch‐enriched fractions prepared from whole peas and dehulled peas contained 73.7% wt and 77.8% wt starch, respectively, a nearly two‐fold enrichment compared with whole peas. The fractions were liquefied and saccharified using industrial α‐amylase and glucoamylase at recommended enzyme loadings. A final ethanol concentration of 11.0% (w/v) was obtained in 48–52 hr, with yields of 0.43–0.48 g of ethanol/g of glucose. Starch present in whole ground peas was also saccharified and fermented, with 97% of the starch fermented when an autoclaving step was included in the liquefaction stage.     

Immunisation against ovine caseous lymphadenitis: correlation between Corynebacterium pseudotuberculosis toxoid content and protective efficacy in combined clostridial-corynebacterial vaccines

Groups of sheep were dosed with vaccines containing Corynebacterium pseudotuberculosis toxoid combined in varying amounts with 5 clostridial antigens. Resistance of the sheep to infection with C pseudotuberculosis was tested at 1, 6 and 12 months after vaccination by infection with pus from ovine lymph glands actively infected with C pseudotuberculosis. The outcome was assessed 3 months after challenge by slaughter and inspection of the sheep for caseous lymphadenitis lesions. Protection was demonstrated by a significant reduction in the proportion of immunised sheep exhibiting lesions compared with control sheep, and by fewer abscesses in affected immunised sheep than in affected control sheep. A positive correlation was found between amount of C pseudotuberculosis toxoid administered and degree of protection obtained. Chromatographically-purified toxoid induced essentially the same protection, suggesting that anti-toxic immunity is the major factor in protection.