海蓬子中高亲和钾离子转运体SbHKT1基因的克隆、表达及生物信息学分析

本研究利用RACE技术从真盐生植物海蓬子中获得了高亲和钾离子转运体SbHKT1基因1647bp完整的ORF框。序列分析结果表明,该基因编码548个氨基酸,分子量为62.10kD,理论等电点为9.33;氨基酸序列中第1个～第35个属信号肽序列,第197个～第537个属离子转运体（TrkH）家族特征序列;该基因编码的蛋白具有10个跨膜结构,N端跨膜区及中部膜上呈现明显的疏水性,C端及中部多个跨膜区呈现强疏水性,符合载体类运输蛋白的特点,因此推测SbHKT1蛋白为跨膜运输蛋白。Blast分析显示该蛋白与碱蓬SsHKT1氨基酸同源性高达77%,与冰叶日中花、赤桉和小麦HKT类蛋白的同源性分别为63%、52%和46%。SbHkt1基因表达存在组织特异性：正常生长条件在根、茎中表达较低,在叶片中几乎看不到表达;在高盐低钾的环境下,各组织表达明显升高,高盐低钾胁迫处理8h,其根部表达处于高峰期;经100μmol/L脱落酸处理4h,根部表达达到最高;干旱胁迫（20%PEG6000）处理2h,根部表达量明显上升。由此推断,该基因参与了植物在高盐低钾、渗透、干旱等非生物胁迫下的生理调控。由于目前已克隆的HKT类蛋白基因多来自非盐生植物,对盐生植物内源HKT基因的研究相对较少,因此,海蓬子内源HKT1基因的全长的获得有助于我们进一步研究该基因在高盐钾饥饿环境下运输钾离子,调节植物体内Na＋/K＋平衡的功能,对于揭示真盐生植物的耐盐机制,将其运用于非盐生植物,培育新的耐盐品种具有一定的意义。     

水库移民安置中水土资源优化配置与生态补偿

水库建设所产生的移民安置问题影响着工程进展、库区与移民安置区的稳定发展,在库区移民实物指标调查与多源数据集成应用基础上对水库移民安置中水土资源进行优化配置与生态补偿,促进库区和移民安置区可持续发展。采用现代信息技术对移民实物指标进行采集、处理与集成应用,建立面向水库移民安置的移民信息系统,为水库淹没影响范围的确定与移民安置实施提供辅助决策支持方法,对库区和移民安置区的水土资源与生态进行综合考虑,以此指导水土资源的优化配置和生态补偿。以陕西省引汉济渭调水工程三河口水库移民安置为应用实例,对采集的三河口水库移民实物指标进行统计分析,通过移民信息系统对实物指标进行空间展示,采用问卷调查确定移民安置方式和移民安置区,提出移民安置区水土资源优化配置与生态补偿措施。在水库移民安置过程中对水资源、土地资源和生态环境进行综合考虑有助于移民安置的科学有序进行,库区和移民安置区后期的稳定与可持续发展,在同类工程中具有一定的指导意义。     

Seasonal Changes of Mineral Nutrients in Fruit and Leaves of ‘Newhall’ and ‘Skagg's Bonanza’ Navel Oranges

In southern Jiangxi province of China, ‘Newhall’(Citrus sinensis Osbeck) navel orange presented a conspicuous symptom of boron (B) deficiency in mature leaves, whereas B deficiency symptoms were not manifested on ‘Skagg's Bonanza’(C. sinensis Osbeck) navel orange. In this study, changes in concentrations of B, calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), and zinc (Zn) were comparatively investigated in the structural parts of the fruit (rind and pulp) and leaves (old leaves from last season and spring-flush leaves from current year) of ‘Newhall’ and ‘Skagg's Bonanza’ navel orange during the growing season. Two peaks of B concentrations were observed in the rind of the two cultivars during fruitlet growth and fruit enlargement, respectively. Boron concentrations were relatively high in the rind during fruitlet growth, and then decreased in both rind and pulp, whereas, during middle and late fruit enlargement significant increases were found for B in both rind and pulp of the two cultivars. Boron concentrations in old leaves of ‘Newhall’ decreased progressively and remained relatively low, whereas that of ‘Skagg's Bonanza’ was relatively high and changed slightly as the season progresses. Both Ca and K concentrations were above the critical threshold values, while their dynamics were reverse to that of B in fruit and leaves during certain times. Old leaf Mg concentrations of samples at 140 days after full bloom from the two cultivars and spring-flush leaves from ‘Newhall’ were below the threshold limit for sufficiency. In addition, Mg in old leaves was much lower from ‘Newhall’ than from ‘Skagg's Bonanza’. Spring-flush leaf concentrations of Mn and Zn and Mn concentrations in old leaves from ‘Newhall’ were relatively lacking during middle and late season, which accelerated the occurrence of B deficiency symptoms on mature leaves of ‘Newhall.’     

山地果园牵引式双轨运输机断绳制动装置设计与试验

针对山地果园牵引式双轨运输机存在钢丝绳松脱或断裂可能引发溜车事故等问题,研制了一种断绳制动装置,描述了载物滑车的总体结构及制动装置关键结构尺寸关系,分析了制动装置制动过程的运动规律。应用SolidWorks建立制动装置简化模型,然后倒入ADAMS/View中建立了制动装置和轨道横梁虚拟样机,虚拟制动试验确定了制动过程的动力学变化规律。动力学仿真与台架试验结果表明,该断绳制动装置的制动成功率为100%,随着装载质量的增大,制动杆与轨道横梁碰撞后的明显回弹次数逐渐减少;碰撞点的应力以及制动杆的回弹距离均随着回弹次数的增大而逐渐减小;仿真结果与试验结果相差极小,验证了仿真过程是正确的。该研究可为山地果园轨道运输机械的安全制动装置设计及后续优化提供参考。     

Impacts of soil erosion on organic carbon and nutrient dynamics in an alpine grassland soil

The impact of soil erosion on the nutrient dynamics in alpine grassland soils is still an essential problem. Selecting a grass-covered hillslope in eastern Tibet Plateau, the cesium-137 (¹³⁷Cs) technique was used to determine the impacts of soil erosion on soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), and total potassium (TK). The ¹³⁷Cs data revealed that there were distinct soil redistribution patterns in different hillslope positions because of the influences of slope runoff, plant coverage and grazing activity. For the upper slope, soil erosion first decreased downward, followed by soil deposition in its lower part. In contrast, for middle and toe slopes, there was an increasing soil erosion along a downslope transect. Across the lower slope, soil erosion showed an irregular variation. Influenced by the selective transport of water erosion, SOC, TN and TP storage decreased with increasing soil erosion in upper, middle and toe slopes. In contrast, SOC, TN and TP storage varied little with soil erosion in the lower slope. On the whole hillslope, TK storage also varied little with soil erosion due to the large amount of potassium elements derived from soil parent materials. Particularly noteworthy was the greatest storage of SOC, TN and TP in the lower slope where most obvious net soil erosion occurred, which is closely related to the humus accumulation combined with gravel separation as well as weathering and pedogenesis of parent rocks induced by soil freeze-thaw.     

基于土壤水分异质性的小麦滴灌试验小区布设方法

科学合理地确定适宜的试验小区规格,对于经济、高效地获取具有较高精度和代表性的试验数据具有重要现实意义.该研究以确定滴灌条件下适宜的试验小区规格为目标,采用幂函数建立土壤含水率方差与不同小区面积之间的相关关系,计算土壤水分异质性指数以表征试验小区内土壤含水率的相关程度;利用土壤含水率异质性指数,采用Hatheway法确定小麦滴灌试验小区适宜的面积和重复数;根据不同方向上土壤含水率异质性指数研究形状对试验结果的影响,确定试验小区适宜的长宽比.结果表明:增大试验小区面积可以提高试验数据的准确性,但不同面积区间数据离散程度的降低幅度变化较大:面积由1 m2增大到50~100 m2,灌水定额30 mm处理田块间土壤含水率方差降低77.4%~82.6%,灌水定额45 mm处理降低78.6%~83.7%,面积从100 m2增加到500 m2,方差降低幅度显著变小,灌水定额30 mm处理降低17.4%,灌水定额45 mm处理降低16.3%;增加试验重复数可以增加试验对处理间土壤含水率差异的区分能力,试验小区面积在50~100 m2时,重复数由2增加到3能检测出的处理间土壤含水率差异由23.2%~26.5%提高到13.1%~15.0%,表明增加试验重复数可以检测出试验处理间更小的土壤含水率差异,提高试验精度.通过研究滴灌试验小区土壤水分异质性指数与滴灌试验小区规格之间的关系,综合考虑试验精度、代表性和田间实际操作得出以下结论:若试验为高、低水2处理时,试验布设宜为3重复,每重复小区面积为50 m2,可在80%的概率下检测出15%的真实差异;若试验为高、中、低3处理时,试验布设宜为3重复,每重复小区面积为100 m2,可在80%的概率下检测出12%的真实差异.滴灌试验小区适宜的形状为沿滴灌带方向布设的长方形,长宽比在1:1到5:1之间可使试验小区所得数据更具代表性.     

Simulating site-specific impacts of climate change on soil erosion and surface hydrology in southern Loess Plateau of China

Proper spatial and temporal treatments of climate change scenarios projected by General Circulation Models (GCMs) are critical to accurate assessment of climatic impacts on natural resources and ecosystems. The objective of this study was to evaluate the site-specific impacts of climate change on soil erosion and surface hydrology at the Changwu station of Shaanxi, China using a new spatiotemporal downscaling method. The Water Erosion Prediction Project (WEPP) model and climate change scenarios projected by the U.K. Hadley Centre's GCM (HadCM3) under the A2, B2, and GGa emissions scenarios were used in this study. The monthly precipitation and temperature projections were downloaded for the periods of 1900–1999 and 2010–2039 for the grid box containing the Changwu station. Univariate transfer functions were derived by matching probability distributions between station-measured and GCM-projected monthly precipitation and temperature for the 1950–1999 period. The derived functions were used to spatially downscale the GCM monthly projections of 2010–2039 in the grid box to the Changwu station. The downscaled monthly data were further disaggregated to daily weather series using a stochastic weather generator (CLIGEN). The HadCM3 projected that average annual precipitation during 2010–2039 would increase by 4 to 18% at Changwu and that frequency and intensity of large storms would also increase. Under the conventional tillage, simulated percent increases during 2010–2039, compared with the present climate, would be 49–112% for runoff and 31–167% for soil loss. However, simulated soil losses under the conservation tillage during 2010–2039 would be reduced by 39–51% compared with those under the conventional tillage in the present climate. The considerable reduction in soil loss in the conservation tillage indicates the importance of adopting conservation tillage in the region to control soil erosion under climate change.     

基于MODIS遥感资料估算高原地表潜热通量

利用2014年夏季青藏高原9个观测站的实测资料,首先分析了夏季高原地区的湍流输送特征,并对MODIS(Moderate-Resolution Imaging Spectroradiometer)地表潜热产品在高原地区的适用性进行检验,进一步在潜热模型中引入MODIS昼夜地表温度来估算研究区的地表潜热,并将估算值与实测值进行对比。结果表明:下垫面以裸土/稀疏植被为主的阿里站能量输送以感热为主,其他观测站的湍流输送则以潜热输送为主;MODIS潜热产品在高原各地区的适用性存在差异,产品在高原东部偏东地区适用性较好,其他地区适用性较差,且高原西部的潜热有效数据大量缺失;而新提出的方法不仅可以弥补高原西部地表潜热的缺失,还可以提高地表潜热的估算精度,估算值与实测值之间相关系数达到0.77,均方根误差仅为29.8 W/m2,相对误差为35.59%;模型给出的高原地区地表潜热区域分布特征与高原的地表覆盖类型吻合较好,说明模型给出的高原地表潜热分布是合理的。     

花生含油量研究进展

含油量是花生重要的品质性状之一,是育种工作者改良花生品质性状的主要目标。笔者简述了花生含油量的组成与比例,回顾了环境因素、营养元素和其它相关性状3个方面的外部因素对花生含油量的影响,总结了人们利用经典遗传学和分子数量遗传学的方法在花生含油量遗传方面的研究进展,并从采用切实可行性的农艺调控措施、协调好紧密相关性状之间的关系、利用现代育种手段辅助选择以及弄清含油量分子层面上的遗传机理等4个方面提出了改良花生含油量的方法。     

水稻插秧机后插式分插机构运动分析与试验

对水稻插秧机后插式分插机构进行了分析。通过秧针端点运动轨迹上的6个特定点,对后插式分插机构的运动学特性作了理论分析和计算机模拟,并利用高速摄像对机构运动学分析进行了试验验证。理论分析和田间试验表明,后插式分插机构的结构参数选择合理,插秧性能优良。