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Atopic dermatitis (AD) and recurrent urticaria (RU) are common immune‐mediated conditions of horses and ponies associated with high morbidity. Effective pharmacological treatment options are limited but identification of the causal allergens allows avoidance strategies and immunotherapy regimens to be employed. Intradermal testing (IDT) is the most widely accepted means of identifying the relevant allergens but there are no published reports of this technique being used in the UK for the investigation of dermatological disease. This study presents the results of testing with a varied panel of allergens in 50 horses with dermatological disease living in the south of England. Intradermal testing was performed in horses presented to The Liphook Equine Hospital for further investigation of AD or RU between June 2002 and March 2009. Allergen selection was based upon availability, results of previous studies, pollen charts and the likelihood of allergens being prevalent in the stable or pasture environment in the south of England. Injection sites were evaluated at 1 h (immediate phase), 4 h (late phase) and 24 h (delayed phase) and skin responses compared to the response generated by the positive control (histamine) at 1 h. Total numbers of positive reactions and numbers of positive reactions to specific allergens were similar in horses with atopic dermatitis and those with urticaria (P = 0.39). There was a statistically significant difference in the number of reactions observed at different time points, with more positive reactions occurring at 1 h than 24 h (P<0.001), and at 4 h than 24 h (P<0.001). Reading the test at 24 h rarely provides additional information. Reaction patterns were similar to those of previous studies performed in other countries with large numbers of positive reactions reported to mites, dusts and insects. Positive reactions were also common to allergens not previously identified as irritants or common allergens in equids; nettle, daisy, dandelion, horse chestnut, cat, cattle, sheep and pigeon. These allergens may be important causes of allergic dermatitis in equids in the UK; however, further studies should be performed in both normal horses and horses with allergic dermatitis to investigate irritant thresholds and validate these findings. Intradermal testing may be shortened from the conventional 24 h to 4 h without significantly affecting the results of the test. 相似文献
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Newton Z. Lupwayi Benjamin H. Ellert Eric Bremer Elwin G. Smith Renee M. Petri Jonathan A. D. Neilson H. Henry Janzen 《Soil Use and Management》2023,39(1):402-414
Variable results have been reported on the effects of crop residue loads on soil microbial properties. We investigated changes in soil bacterial composition, β-glucosidase enzyme activity and nutrient bioavailability in response to wheat residue loading. The treatments included three levels of above-ground wheat residues (removed, retained or supplemented), with or without fertilizer N. Bacteroidetes, Firmicutes and Verrucomicrobia (the first two are copiotrophs) were less abundant where residues were removed than where residues were retained or supplemented, but the reverse was true for Actinobacteria, Cyanobacteria, Chloroflexi and Nitrospirae (all oligotrophs, although some Actinobacteria can be copiotrophic). Actinobacteria were also less abundant where fertilizer N was applied, and the abundances of their genera (including Arthrobacter and Mycobacterium) increased where residues were removed, confirming that they were oligotrophic in this study. β-diversity showed similar differences in the bacterial community structures because of residue management, but α-diversity was not affected by residue management or N fertilizer. β-glucosidase enzyme activities increased as C inputs increased with residue manipulation and N fertilizer. The enzyme activities increased with increasing residue loading in the 0–15 cm soil depth, but decreased with soil depth. Soil K supply increased with increasing residue loading, but nitrate-N supply was highest with residue retention. These results demonstrate remarkable resilience of soil microbial functioning under a wide range of crop residue inputs, without adverse effects on enzyme activity attributable to inorganic N fertilizer. The increasing β-glucosidase activity with increasing residue loading probably explains why crop residue return does not always increase soil C stocks. 相似文献
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REASONS FOR PERFORMING STUDY: The proposed biological mechanisms for exercise-induced pulmonary haemorrhage (EIPH) are many and varied. Better knowledge of risk factors should lead to achievable measures to reduce the incidence. OBJECTIVES: To identify risk factors associated with epistaxis following racing in UK Thoroughbreds, to gain possible insights into the pathogenesis of the condition and to investigate the association between epistaxis and race finishing position. METHODS: The association of epistaxis occurring on UK racecourses between 1996 and 1998 with a wide range of race-, horse- and start-level variables was examined in multivariable mixed effect logistic regression analyses. Four multivariable analyses were conducted, one for all race types considered collectively and one each for flat, hurdle and steeplechase race types considered separately. RESULTS: Risk of epistaxis was significantly increased for hurdle and steeplechase race types compared to both flat and National Hunt flat races. In 3 of the 4 final models, there was a significant biological trend for increasing risk of epistaxis with increasing ground hardness ('going') and accumulated years spent racing. However, in flat races epistaxis was such a rare outcome (0.33 cases per 1000 starts) that this subset analysis had insufficient power to measure the detectable effect of 'going' as statistically significant. Horses with epistaxis were significantly more likely to have a poorer finishing position than those without blood at the nostrils. CONCLUSIONS: Findings were consistent with the theory that locomotory impact-induced trauma contributes to exercise-induced epistaxis. Further validation of this hypothesis through application of similar methods to endoscopically visible EIPH and through biomechanical studies is warranted. POTENTIAL RELEVANCE: Knowledge of significant risk factors should allow formulation of practical measures, such as track watering, to reduce the risks of EIPH and epistaxis in racehorses. 相似文献
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REASONS FOR PERFORMING STUDY: Clinicians are often asked to guide owners and trainers over the relative advantages and disadvantages of equine castration performed in either the standing horse with an open unsutured scrotal wound with healing by second intention, or a recumbent horse under general anaesthesia in aseptic conditions, with sutured scrotal skin allowing primary wound closure. OBJECTIVES: To identify types and frequency of complications following the 2 differing approaches, and to compare the financial cost associated with each procedure, based on practice charges. METHODS: Veterinary expenses of 217 horses castrated by a Newmarket equine veterinary practice over an 18-month period were analysed. Of these, Group 1 (n = 121) were castrated standing and nonsutured by one of 2 ambulatory clinicians and Group 2 (n = 96) castrated in recumbency, in aseptic equine hospital conditions. RESULTS: Group 1 had a complication prevalence of 22% with no mortalities, and Group 2 a significantly lower complication prevalence of 6% (P = 0.001) with a mortality rate of 1%. The financial cost of Group 1, without complications, was approximately one-third of the cost of uncomplicated Group 2. However, the cost of Group 1 with complications increased to approximately two-thirds of the cost of an uncomplicated Group 2 castration. CONCLUSIONS: Even though the complication prevalence for Group 1 castrations leaving an open scrotal wound was significantly higher than for a recumbent horse with a sutured scrotal wound in a hospital, the average cost of Group 1 was still less, even taking into account the additional follow-up costs associated with treating such complications. POTENTIAL RELEVANCE: This report provides a benchmark for the outcome of 2 methods of castration based on a database obtained from particular circumstances within the practice involved. Further studies are required to corroborate and take into account future development in surgical and anaesthetic techniques. 相似文献