Potential of New Strains of Marine and Inland Saline-Adapted Microalgae for Aquaculture

Several problems currently limit the reliability of algal cultivation systems for the production of aquaculture feeds. Many of these problems may be eventually solved through research in culture system design and operation. However, research into the isolation and development of new strains of microalgae may also directly lead to improved system performance.
Through a collection and screening program aimed at isolating microalgae tolerant to high light, high salinity, and high temperatures, we have been able to isolate several new strains with enhanced production potential (30-35 g/m²/d). The overall range of environmental tolerance exhibited by these strains may result in enhanced culture stability, leading to the high production rates.
The estimated costs of producing strains with these high production rates range from $1.18 to S1.71 per kg (unharvested), depending on the size of the production system. Total production costs for harvested algae (15% solids algal cell paste) range from $1.63 to $2.45 per kg. The major costs for producing harvested algae include the cost of CO₂, as well as capital and operating costs for the harvesting systems.     

Metalaxyl and other fungicides for control ofPhytophthora infestans on potato in northwest Washington State

In northwest Washington State, fungicidal control of late blight caused byPhytophthora infestons was evaluated for three years. During 1977, a high rainfall year with supplemental sprinkler irrigations, all metalaxyl and chlorothalonil treatments reduced tuber rot at harvest, but only metalaxyl extended control of tuber rot after 4 months’ storage. During 1978, a high rainfall year, one application of metalaxyl sprayed over or dusted on potato seed pieces at planting or applied five times to foliage at 2 or 4 wk intervals gave excellent blight control equal to that with chlorothalonil applied to foliage every 2 wk. In 1979, a low rainfall year,P. infestons did not kill plants in untreated plots until October. Five applications of metalaxyl sprayed every 2 wk on foliage did not control foliage blight. However, metalaxyl reduced foliage blight when dusted on cut potato seed pieces. Mancozeb sprayed every 2 wk also gave excellent control. The possibility that metalaxyl applied to foliage is effective in late blight control only when washed into the soil by rainfall or irrigation for uptake by roots is discussed. Yields and percent U.S. No. 1 grade tubers were not significantly affected by any treatment in these 3-year trials.     

Effect of irrigation on near-infrared (NIR) based prediction of mango maturity

This study investigated the effect of irrigation on the ability of near-infrared (NIR) measurements to predict maturity stage of mango. Fruits from irrigated and non-irrigated trees were sampled on six dates during the final three weeks of development and subjected to NIR and conventional laboratory analyses. Spectral assessment showed differentiation between irrigated and non-irrigated fruits on earlier dates, which was not evident later on. NIR measurements of irrigated samples correlated well to dry matter, this was not the case for non-irrigated samples (r = 0.80 compared to 0.57, respectively), while the reverse was true for acidity with r increasing from 0.55 to 0.85 between irrigated and non-irrigated. Second derivative spectra of all samples best correlated with acid content (r = 0.73). Although dry matter was previously proposed as the most suitable parameter for NIR calibration, this study suggests acidity might be an appropriate harvesting index when considering irrigation effects. Interestingly, NIR technology has been found to adequately predict acidity in other fruits, but results of this study require additional investigation.     

南昌市城-郊-乡梯度五种森林类型表层土壤磷素分布格局(英文)

城市化所改变的土壤利用方式将显著地影响土壤磷素(P)含量水平。本研究沿地处中亚热带地区的南昌市选取灌丛、松林、针阔混交林、常绿阔叶林和毛竹林为研究对象,采用改进Hedley的P素连续分级法分析了土壤全P及各功能P形态的分布规律。结果表明:森林表层土壤全P和可提取态P的含喇均表现为城区(0.71g·kg-1和378.50mg·kg-1)明显高于郊区(0.30g·kg-1和150.74mg·kg-1)和农村(0.31g·kg-1和147.38mg·kg-1)(p0.05)。在树脂P、NaHCO3-P、NaOH-P、声波P和HCl-P等5种功能P形态中,城区森林土壤HCl-P的相对含度最高,为36%,明显高于其郊区相对含量为8%年农村相对含量为6%的水平。而郊区和农村地区,土壤NaOH-P是其主要的存在形态,其相对含量分别达到41%和50%。可见,城区土壤P积累将影响城市森林生态系统的P循环,且其高含量的HCl-P可能加速P在城区水生系统的富集。图3表2参32。     

TGA modeling of the thermal decomposition of CCA treated lumber waste

 To guide the development of thermal decomposition methods for disposal of CCA treated wood, reactions during the thermal decomposition of CCA treated wood were modeled using thermogravimetric analysis (TGA), with special focus placed on arsenic volatilization. Simple inorganic compounds, such as As₂O₅, CuO, and Cr₂O₃, were used to model the thermal behavior of the inorganics in CCA treated wood. In air and nitrogen, arsenic (V) oxide began to volatilize at 600 °C during temperature ramps at 5 °C/min. During a 5 °C/min ramp in a hydrogen mix, arsenic (V) oxide began decomposition at 425 °C. Arsenic volatile loss from CCA treated wood can depend strongly on the gases produced by wood thermal decomposition. In the presence of As₂O₅, chromium (III) oxide and copper (II) oxide formed arsenates in air and nitrogen. Chromium arsenates began decomposition as low as 790 °C. This suggested that chromium arsenates in CCA treated wood formed during original preservative fixation may decompose as low as 790 °C. Copper arsenates were stable up to 900 °C in air, but showed only a limited range of stability in nitrogen. Depending on process conditions, the formation of copper arsenates may limit arsenic loss during thermal decomposition of CCA treated wood up to 900 °C. The thermal decomposition of inorganic oxides was influenced by interactions with wood and wood decomposition products. In a dry YP sawdust/As₂O₅ mix, arsenic (V) oxide volatilized at 370 °C during inert pyrolysis at 5 °C/min and at 320 °C during smoldering combustion at 5 °C/min. Thermal dwells of a dry YP/As₂O₅ mix showed no arsenic loss at 250 °C, but significant loss occurred during higher temperature dwells. During inert pyrolysis at 5 °C/min, the formation of complexes and hydrates were shown to prevent arsenic loss up to 400 °C. Received 14 July 1999     

Wheat field erosion rates and channel bottom sediment sources in an intensively cropped northeastern Oregon drainage basin

Sediment tracers were used to quantify erosion from cultivated fields and identify major source areas of channel bottom sediment within the Wildhorse Creek drainage, an intensively cropped tributary of the Umatilla River in northeastern Oregon, USA. Available data indicated that Wildhorse Creek was one of the largest sediment yielding tributaries of the Umatilla River. Carbon, nitrogen and the nuclear bomb‐derived radionuclide ¹³⁷Cs were used as tracers to fingerprint sediment sources. Sediment was collected from the stream bottom and active floodplain and compared to samples from cultivated fields and channel banks. Samples were characterized on the basis of tracer concentrations and a simple mixing model was used to estimate the relative portion of bottom sediment derived from cultivated surface and channel banks. The results indicate that the amount of bottom sediment derived from cultivated surface sources was less than 26 per cent for the 1998 winter season, although this estimate has a high margin of error. Cesium‐137 was also used to estimate surface erosion from three cultivated fields in the watershed. Annual estimates of erosion since 1963 from the three sampled fields were from 3 to 7ċ5 t ha⁻¹ yr⁻¹. For the 1998 season, it appears that most channel‐bottom sediment was of subsurface origin with much of it likely coming from channel and gully banks indicating that significant reductions in sediment in Wildhorse Creek might be accomplished by the stabilization of eroding riparian areas and swales on the lower slopes of agricultural fields. Published in 2004 by John Wiley & Sons, Ltd.     

Aircraft-applied sulfur for control of powdery mildew (erysiphe cichoracearum) on potato in washington state

Aircraft-applied dust and liquid sulfur sprays were evaluated for control of powdery mildew (Erysiphe cichmacearumDC), on rill irrigated potatoes (cv. Russet Burbank). Either form of sulfur applied weekly or biweekly usually controlledE. chichoracearum through September, but not in October. Significant yield increases were obtained in only two of five trials. The small differences in disease severity between weekly and biweekly applications of sulfur were not significant. Sulfur gave very little control if applied after infection was established. Applications of liquid sulfur, the formulation less likely to cause environmental problems, should be started at the first trace of stipples on stems and continued biweekly throughout the season.     

Imaging of Plasmodium liver stages to drive next-generation antimalarial drug discovery

Most malaria drug development focuses on parasite stages detected in red blood cells, even though, to achieve eradication, next-generation drugs active against both erythrocytic and exo-erythrocytic forms would be preferable. We applied a multifactorial approach to a set of >4000 commercially available compounds with previously demonstrated blood-stage activity (median inhibitory concentration < 1 micromolar) and identified chemical scaffolds with potent activity against both forms. From this screen, we identified an imidazolopiperazine scaffold series that was highly enriched among compounds active against Plasmodium liver stages. The orally bioavailable lead imidazolopiperazine confers complete causal prophylactic protection (15 milligrams/kilogram) in rodent models of malaria and shows potent in vivo blood-stage therapeutic activity. The open-source chemical tools resulting from our effort provide starting points for future drug discovery programs, as well as opportunities for researchers to investigate the biology of exo-erythrocytic forms.     

A randomized, double-blind, placebo-controlled trial to investigate the efficacy and safety of a Chinese herbal product (P07P) for the treatment of canine atopic dermatitis

A randomized, double-blind, placebo-controlled trial of P07P, a product derived from a traditional Chinese herbal remedy, was undertaken in 50 dogs with atopic dermatitis. Owners recorded a daily itch score for 4-14 days before treatment and during treatment. Packets of powder containing P07P or placebo were added to the food once daily for 8 weeks. Dogs were assessed for erythema, surface damage, overall coat condition and seborrhoea by the same investigator, as well as for pruritus and general demeanour, at 0 (visit 2), 28 (visit 3) and 56 (visit 4) days of treatment or at withdrawal. Investigator and owner assessments of response were recorded after 28 and 56 days of treatment or at withdrawal. The predefined primary outcome measure was the owners' assessment of response at the end of treatment. Nine of the 24 dogs (37.5%) in the P07P group but only 3 of the 23 dogs (13%) in the placebo group were considered to have improved, but this difference was not statistically significant (P = 0.09). There was a significantly higher withdrawal rate due to worsening of condition in the placebo group (P = 0.04). Mean daily itch score in the second 28-day period of the study was significantly higher than baseline in the placebo group (P = 0.01) but not in the P07P group (P = 0.30). Pruritus scores showed a significant deterioration from baseline at the final visit in the placebo group (P = 0.01) but not in the P07P group (P = 1.00). There was a significant difference between the groups in change from baseline in erythema score at visit 3 (P = 0.05). There were no significant differences (P > 0.05) in surface damage, seborrhoea, overall coat condition and general demeanour scores within or between the groups throughout the study. The product was well tolerated with no severe or serious adverse events recorded. P07P may be beneficial as a novel nonsteroidal therapy for the management of dogs with atopic dermatitis.