小麦淀粉颗粒蛋白-1突变体挖掘及其相关农艺性状和品质特点的分析

小麦淀粉颗粒蛋-1(starch granule protein 1,即SGP-1)突变体是选育高直链淀粉含量、高抗性淀粉小麦品种的重要基础材料。SGP-1突变缺失,使胚乳中支链淀粉侧链的延伸受阻,从而使胚乳表观直链淀粉含量相对升高,所制食品的抗性淀粉含量也相应提高,具有重要的保健意义。本研究主要是利用SDS-PAGE方法,检测307份国内外农家种、育成种、高代品系及国内外黑小麦材料的SGP-1(SGP-A1、SGP-B1和SGP-D1)蛋白组成,进而发掘SGP-1突变体;并对突变体材料进行相关农艺性状和品质性状特点的分析,为育种利用及品质改良提供参考。结果共检测到4份SGP-1突变体,均为冬性材料,其中国外材料3份(CH62444、EAP74961和Amigo)、高代品系1份(05黑初8),分别为SGP-A1单缺体,SGPA1和-D1双缺体,SGP-A1和-B1双缺体,以及SGP-A1单缺体。在主要农艺性状方面,EAP74961、05黑初8和Amigo抽穗期与石4185(CK)相近,而CH62444则抽穗较晚,并且3份国外材料植株均较高。这4份突变体材料麸皮中的非水溶性戊聚糖含量相对于水溶性戊聚糖占绝对优势,而胚乳中非水溶性戊聚糖含量与水溶性戊聚糖含量则较为接近,但CH62444、Amigo和05黑初8麸皮中的总戊聚糖含量较高(分别为14.33%,15.20%和12.91%),而EAP74961面粉中的总戊聚糖含量(1.34%)较高。在淀粉组分含量方面,4份突变体材料差异不大。     

河南卫河流域脆弱性评价

结合卫河自身特点和地理环境建立13项指标体系,运用基于熵权的模糊数学模型对其脆弱性进行评价。结果表明,鹤壁的脆弱性为0.69,焦作为0.55,新乡为0.39,安阳为0.36,濮阳为0.23。最后针对评价结果分析了原因,提出了合理性建议和措施。     

汽车尾气净化催化剂的自动配制技术研究

使用可编程控制器对汽车尾气过滤载体所需要的净化催化剂溶液的工艺过程进行自动化操作，系统由S7-200通过软件和硬件对各参数实现在线检测和控制。经生产现场调试使用，自动控制效果良好，达到预期的目的和要求，用户满意。     

黑蜣(Bess beetle)纤维素酶的组分及其特性分析

以30日龄的黑蜣幼虫为材料,对其整体匀浆后组织液中的纤维素酶组成及活性进行了分析。结果表明:黑蜣体内存在内切β-1,4-葡聚糖酶(Cx酶)、β-葡萄糖苷酶;β-葡萄糖苷酶和Cx酶的最适宜温度为分别为40℃和50℃;Cx酶和β-葡萄糖苷酶分别在pH值为6.4和5.2时具有最大的活力;β-葡萄糖苷酶和Cx酶的最适宜反应时间为80 min。Cx酶具有比β-葡萄糖苷酶更强的温度适应性,在30～50℃温度范围内可维持最大酶活力的66.20%。     

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.     

Effect of Alfalfa Flavonoids on Growth Performance and Serum Indexes of Sheep

This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%.     

农业机械购置补贴网络系统数据库设计与应用

农机购置补贴是国家支农惠农的政策之一.对农机购置补贴系统的数据库功能进行分析,提出农机补贴数据表关系模型,并对系统安全维护进行论述.数据库运用在农业购置补贴网络系统中,满足农机管理部门对农机补贴数据事务性管理,为农机补贴政策决策提供实时和有效的数据分析.     

Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.     

补喂支链氨基酸、肌酸对伊犁马血浆生化指标的影响

【目的】研究补喂支链氨基酸(BCAA)、肌酸(Cr)对伊犁马血浆抗氧化指标、激素水平、肌酸、葡萄糖浓度以及乳酸、尿酸和肌酐含量的影响,为支链氨基酸、肌酸作为伊犁马有效的营养添加剂提供参考数据。【方法】选取年龄3~5岁(平均4岁),体重390~514 kg(平均457±50 kg),经过良好训练的伊犁马公马12匹进行试验。分为3组,每组4匹,分别为对照组、试验Ⅰ组和试验Ⅱ组。对照组补喂不添加任何添加物的补喂料,试验Ⅰ组补喂添加BCAA的补喂料,试验Ⅱ组补喂添加Cr的补喂料。分别在补饲的0、1、14、21和31 d补喂后4 h颈静脉采集血液。采用试剂盒法测定相关指标。【结果】补喂Cr能够明显提高T-AOC能力,Cr补喂组与对照组差异显著(P<0.05),特别是在14和31d比对照组提高了21.79%和25.68%;补喂BCAA降低试验期间MDA的含量;对于血浆中的胰岛素、胰高血糖素和皮质醇等3种激素水平,肌酸、葡萄糖、乳酸、尿酸和肌酐的浓度与对照组相比差异不显著(P>0.05)。【结论】补喂BCAA可以降低脂质过氧化产物MDA的浓度;补喂Cr可以提高SOD的活力和提高伊犁马血浆的T-AOC能力;补喂BCAA、Cr对伊犁马血浆中的胰岛素、胰高血糖素和皮质醇等3种激素的水平均无显著影响;对浆中肌酸、葡萄糖的浓度均无显著影响;对血浆中乳酸、尿酸和肌酐的含量均无显著影响。