高产优质早熟玉米杂交种豫审濮单3号的选育及应用

濮单3号(P9734)是河南省濮阳农科所1995年育成,组合为P97×9212.该组合属中早熟品种,需≥10℃积温2 300℃·d左右.1995～2000年在各级产量试验中表现高产、稳产、优质、多抗.适宜在我国黄淮及东华北春玉米产区种植.种植密度以每公顷5.25～6.00万株为宜,高产攻关田可种植到每公顷6.75万株左右。     

3种外源物质对苹果幼树新梢抗寒性的影响及评价

为了有效预防霜冻对苹果新梢的为害,比较了不同外源物质处理对苹果幼树新梢抗寒性的影响.以2 a生嘎拉/M9T337苹果幼树为试材,从萌芽期开始进行喷施外源物质处理,试验喷施物质设清水(CK)、200 mg/L的水杨酸溶液(SA)、2 g/L的CaCl2溶液以及不同浓度的芸苔素内酯水剂(BR 750倍液、1500倍液和3000倍液)共计6个处理,每7 d喷施1次,连喷3次.第3次喷施外源物质后第10天,剪取新梢分别进行4、0、-2和-4℃的低温胁迫,取叶片测定抗寒相关生理指标;利用各处理不同温度胁迫下叶片相对电导率拟合的Logistic方程计算出半致死温度(LT50),并利用隶属函数法对各外源物质处理的枝条抗寒性进行综合评价.结果表明:BR 1500倍液、2 g/L的CaCl2溶液和200 mg/L的SA溶液处理能有效降低苹果叶片相对电导率,明显提高叶片脯氨酸和可溶性糖的含量以及SOD、POD和CAT的活性;LT50分别为-3.92、-6.79和-3.78℃;抗寒性综合评价均为中抗.     

基于价值星系的企业成本管理研究

在知识经济占主导的当今社会,企业要不断加强对新知识的学习,而价值星系作为一种全新的价值创造模式,为企业所用是顺应时代潮流、符合时代要求的。文章通过对价值链与价值星系的对比,从而分析出价值星系的优势,并在此基础上提出基于价值星系的成本管理新模式。     

仿刺参体壁表皮再生组织学和超微结构观察

本研究采用手术方法刮掉仿刺参(Apostichopus japonicus Selenka)体壁表皮,将受伤仿刺参放入海水中饲养,通过光学显微镜和透射电镜观察再生表皮的组织学、超微结构变化.表皮再生开始于手术后24 h,创面中央新生出表皮,结缔组织中出现扩增细胞.再生48 h,创面有新生角质层出现,上皮细胞单层.再生72 h,上皮细胞仍保持单层,角质层形成清晰可见,扩增细胞开始聚集.表皮再生96 h,角质层增厚,达到正常体壁角质层的厚度,角质层表面形成许多突起,上皮细胞达2～3层,扩增细胞聚集成细胞团,从结缔组织中迁移至表皮.再生13 d,扩增细胞团萎缩.再生19 d,组织基本恢复至正常形态,扩增细胞经分化后已均匀分布到新生表皮层,细胞团消失,上皮细胞排列有序,结缔组织结构疏松,质地均匀.     

Ecological scale and seasonal heterogeneity in the spatial behaviors of giant pandas

We report on the first study to track the spatial behaviors of wild giant pandas (Ailuropoda melanoleuca) using high‐resolution global positioning system (GPS) telemetry. Between 2008 and 2009, 4 pandas (2 male and 2 female) were tracked in Foping Reserve, China for an average of 305 days (± 54.8 SE). Panda home ranges were larger than those of previous very high frequency tracking studies, with a bimodal distribution of space‐use and distinct winter and summer centers of activity. Home range sizes were larger in winter than in summer, although there was considerable individual variability. All tracked pandas exhibited individualistic, unoriented and multiphasic movement paths, with a high level of tortuosity within seasonal core habitats and directed, linear, large‐scale movements between habitats. Pandas moved from low elevation winter habitats to high elevation (>2000 m) summer habitats in May, when temperatures averaged 17.5 °C (± 0.3 SE), and these large‐scale movements took <1 month to complete. The peak in panda mean elevation occurred in Jul, after which they began slow, large‐scale movements back to winter habitats that were completed in Nov. An adult female panda made 2 longdistance movements during the mating season. Pandas remain close to rivers and streams during winter, possibly reflecting the elevated water requirements to digest their high‐fiber food. Panda movement path tortuosity and first‐passage‐time as a function of spatial scale indicated a mean peak in habitat search effort and patch use of approximately 700 m. Despite a high degree of spatial overlap between panda home ranges, particularly in winter, we detected neither avoidance nor attraction behavior between conspecifics.     

Construction of siArid2 recombinant adenovirus and its effect on proliferation of hepatocarcinoma cells

AIM: To observe the effects of Arid2gene on cell proliferation and cell cycle by interference of endogenous Arid2 expression in hepatoma cells. METHODS: Three pairs of shRNA targeting Arid2gene were cloned into a shuttle vector to construct recombinant adenovirus plasmids. HEK293 cells were transfected with the recombinant adenovirus plasmids. After several rounds of the package and amplification, the high-titer adenoviruses AdsiArid2-1~3 were obtained. To verify the inhibitory effects of AdsiArid2 adenoviruses, Western blotting was used to detect the endogenous Arid2 protien expression in SMMC-7721 cells. Cell growth and cell cycle analysis were carried out by MTS assay and flow cytometry. RESULTS: High- titer recombinant adenovirus of siArid2 were successfully obtained, and named AdsiArid2-1~3, among which the AdsiArid2-3 had the best inhibitory effects. MTS assay showed that the absorbance values at 490 nm were increased at 72 h and 96 h after transduction compared with the mock and Adsicontrol groups. These data indicated that knockdown of Arid2 promoted the proliferation rate of SMMC-7721 cells(P<0.05). Moreover, the flow cytometry analysis revealed that the G1-phase distribution at 72 h in AdsiArid2 group was lower than that in mock group and Adsicontrol group. In contrast, the S-phase distribution in AdsiArid2 group was much higher than that in mock group and Adsicontrol group. CONCLUSION: The recombinant plasmids and recombinant adenovirus were successfully constructed. shRNA-mediated knockdown of Arid2 promotes the proliferation and the transition from G1 phase to S phase of hepatoma cells.     

温度对红菜薹贮藏效果的影响

针对红菜薹不耐贮藏的难题,将红菜薹分别置于0、5、10、20℃条件下贮藏,测量其呼吸强度,并进行综合感官评价,研究了不同温度对3个品种红菜薹贮藏效果的影响。研究结果表明,红菜薹最适贮藏温度为5~10℃,相同贮藏条件下,红菜薹品种耐贮性从大到小依次为:SYHYZHJHB。     

Effect of puerarin injection on epithelial-mesenchymal transition in renal tubular epithelial cells of diabetic KKAy mice

AIM:To observe the effect of puerarin injection on the expression of epithelial-mesenchymal transition-related proteins in KKAy mice with renal interstitial fibrosis (RIF). METHODS:Sixteen KKAy mice were randomly divided into model group (n=8) and puerarin injection treatment group (n=8), and 8 C57BL/6J mice were used as normal controls. The mice in treatment group were intraperitoneally given puerarin injection from the 14th week. The blood glucose levels were observed on a daily basis. The mice were sacrificed at the 24th week.The renal pathological changes were observed under light microscope. The expression of α-smooth muscle actin (α-SMA), transforming growth factor β1 (TGF-β1) and transforming growth factor β type I receptor (TGF-β-RI) in the renal tissues were examined by immunohistochemical staining. RESULTS:Fibrosis was found in the KKAy mice of model group, while the mice in treatment group showed a slight increase in renal interstitium. Treatment with puerarin injection decreased the protein expression levels of α-SMA, TGF-β1 and TGF-β-RI in the kidney tissues as compared with those in model group. CONCLUSION: Puerarin injection reduces the expression of α-SMA, and restrains the protein expression of TGF-β1 and TGF-β-RI in the kidney tissue of KKAy mice. These changes may inhibit and reverse the process of epithelial-mesenchymal transition, thus delaying the occurrence and development of RIF.     

基于水量平衡原理的畦灌下渗水形状系数变化规律研究

以往水量平衡模型应用中,通常认为在同一次灌水过程中的下渗水形状系数为恒定值,使模型精度受到一定影响。基于此,本试验以水量平衡原理为基础,结合量纲分析,研究了畦灌过程中的下渗水形状系数变化规律并提出了估算其值的经验计算式。结果表明,同一次畦灌过程中下渗水形状系数并非恒定值,而是随灌水时间和水流推进距离等因素变化的。结合已有文献资料验证,结果表明采用水量平衡模型模拟畦灌水流运动过程,同时考虑动态的地表储水形状系数和下渗水形状系数取值,可有效提高模型的计算精度。同时也表明采用本文所建经验计算式估算畦灌下渗水形状系数值是可靠的。     

光照和温度对旱地小麦叶面积指数的影响

为研究旱地小麦叶片生长规律,了解气候变化对小麦叶面积指数的效应,设计回归正交组合试验,并运用APSIM模型模拟光照和温度变化对小麦叶面积指数的影响。结果表明：APSIM模型对小麦叶面积指数的模拟有较高精度。小麦叶面积指数与光照和温度分别呈开口向下和向上二次抛物型变化。在本试验范围内,叶面积指数与光照呈二次抛物线下降型变化,但当光照未越过临界点时,光照成正效应,表明在最适宜条件下光照的合理增加可促进小麦生长;当日均气温高于7.73 ℃,温度成正效应,说明温度合理的升高,有助于小麦叶面积指数在一定生长阶段平稳在较大值;光照和温度同时升高时,光照下降效应显著于温度正效应。