全文获取类型
收费全文 | 5477篇 |
免费 | 343篇 |
国内免费 | 629篇 |
专业分类
林业 | 449篇 |
农学 | 477篇 |
基础科学 | 311篇 |
787篇 | |
综合类 | 2298篇 |
农作物 | 362篇 |
水产渔业 | 257篇 |
畜牧兽医 | 838篇 |
园艺 | 360篇 |
植物保护 | 310篇 |
出版年
2024年 | 40篇 |
2023年 | 106篇 |
2022年 | 285篇 |
2021年 | 317篇 |
2020年 | 246篇 |
2019年 | 262篇 |
2018年 | 194篇 |
2017年 | 293篇 |
2016年 | 232篇 |
2015年 | 335篇 |
2014年 | 315篇 |
2013年 | 387篇 |
2012年 | 474篇 |
2011年 | 440篇 |
2010年 | 425篇 |
2009年 | 353篇 |
2008年 | 343篇 |
2007年 | 322篇 |
2006年 | 248篇 |
2005年 | 192篇 |
2004年 | 109篇 |
2003年 | 70篇 |
2002年 | 72篇 |
2001年 | 73篇 |
2000年 | 64篇 |
1999年 | 39篇 |
1998年 | 40篇 |
1997年 | 31篇 |
1996年 | 28篇 |
1995年 | 19篇 |
1994年 | 18篇 |
1993年 | 23篇 |
1992年 | 15篇 |
1991年 | 10篇 |
1990年 | 9篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1962年 | 1篇 |
排序方式: 共有6449条查询结果,搜索用时 15 毫秒
31.
32.
CHEN Tian-tian CHENG Fu-liang ZHANG Jian-mei NIE Zhao-jing FANG Dong FAN Mei-na LI Fu-hui CHENG He-gang 《中国畜牧兽医》2015,42(9):2474-2480
The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea. 相似文献
33.
WANG Meng-zhu LIU Yan-feng WANG Wen-qi NIE Cun-xi TANG Shu-zhen ZHANG Wen-ju 《中国畜牧兽医》2015,42(9):2345-2351
This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%. 相似文献
34.
35.
生态系统服务功能是生态系统与生态过程所形成及所维持的人类赖以生存的自然环境条件与效用,对其服务价值进行货币化评价,可以推动自然资源可持续利用,促进区域生态环境保护。以达里诺尔湖湿地为研究对象,结合达里诺尔湖湿地生态系统的结构、特征及其生态过程特点,通过资料搜集与实地调研,运用资源经济学理论和方法,对达里诺尔湖湿地生态系统服务价值进行了划分。构建了达里诺尔湖湿地生态系统服务价值评价指标体系,并对其进行了价值评价。评价结果显示达里诺尔湖湿地的服务价值为93.70×104万元,其中直接使用价值为2.19×104万元,占比为2.36%,间接使用价值为91.51×104万元,占比为97.66%。各项生态系统服务功能中以调节空气湿度价值最大,为82.76×104万元,占总服务价值的88.32%。 相似文献
36.
37.
38.
Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events
To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border(RB) and left border(LB) regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head(RB-to-RB) concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events. 相似文献
39.
40.
Junfeng Wang Yujie Shi Yunna Ao Dafu Yu Jiao Wang Song Gao Johannes M. H. Knops Chunsheng Mu Zhijian Li 《Journal of Agronomy and Crop Science》2019,205(6):554-561
Extreme drought events can directly decrease productivity in perennial grasslands. However, for rhizomatous perennial grasses it remains unknown how drought events influence the belowground bud bank which determines future productivity. Ninety‐day‐long drought events imposed on Leymus chinensis, a rhizomatous perennial grass, caused a 41% decrease in the aboveground biomass and a 28% decrease in belowground biomass. Aboveground biomass decreased due to decrease in both the parent and the daughter shoot biomass. The decreases in daughter shoot biomass were due to reductions in both the shoot number and each individual shoot weight. Most importantly, drought decreased the bud bank density by 56%. In addition, drought induced a bud allocation change that decreased by 41% the proportion of buds that developed into shoots and a 41% increase in the buds that developed into rhizomes. Above results were supported by our field experiment with watering treatments. Thus, a 90‐day‐long summer drought event decreases not only current productivity but also future productivity, because the drought reduces the absolute bud number. However, plasticity in plant development does partly compensate for this reduction in bud number by increasing bud development into rhizomes, which increases the relative allocation of buds into future shoots, at the cost of a decrease in current shoots. 相似文献