全文获取类型
收费全文 | 2942篇 |
免费 | 177篇 |
国内免费 | 305篇 |
专业分类
林业 | 228篇 |
农学 | 155篇 |
基础科学 | 115篇 |
349篇 | |
综合类 | 1426篇 |
农作物 | 181篇 |
水产渔业 | 152篇 |
畜牧兽医 | 468篇 |
园艺 | 227篇 |
植物保护 | 123篇 |
出版年
2024年 | 29篇 |
2023年 | 70篇 |
2022年 | 171篇 |
2021年 | 162篇 |
2020年 | 129篇 |
2019年 | 118篇 |
2018年 | 101篇 |
2017年 | 163篇 |
2016年 | 108篇 |
2015年 | 160篇 |
2014年 | 157篇 |
2013年 | 197篇 |
2012年 | 258篇 |
2011年 | 245篇 |
2010年 | 248篇 |
2009年 | 204篇 |
2008年 | 199篇 |
2007年 | 196篇 |
2006年 | 145篇 |
2005年 | 99篇 |
2004年 | 76篇 |
2003年 | 39篇 |
2002年 | 53篇 |
2001年 | 40篇 |
2000年 | 41篇 |
1999年 | 6篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 2篇 |
1992年 | 2篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1981年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有3424条查询结果,搜索用时 15 毫秒
11.
小麦淀粉颗粒蛋-1(starch granule protein 1,即SGP-1)突变体是选育高直链淀粉含量、高抗性淀粉小麦品种的重要基础材料。SGP-1突变缺失,使胚乳中支链淀粉侧链的延伸受阻,从而使胚乳表观直链淀粉含量相对升高,所制食品的抗性淀粉含量也相应提高,具有重要的保健意义。本研究主要是利用SDS-PAGE方法,检测307份国内外农家种、育成种、高代品系及国内外黑小麦材料的SGP-1(SGP-A1、SGP-B1和SGP-D1)蛋白组成,进而发掘SGP-1突变体;并对突变体材料进行相关农艺性状和品质性状特点的分析,为育种利用及品质改良提供参考。结果共检测到4份SGP-1突变体,均为冬性材料,其中国外材料3份(CH62444、EAP74961和Amigo)、高代品系1份(05黑初8),分别为SGP-A1单缺体,SGPA1和-D1双缺体,SGP-A1和-B1双缺体,以及SGP-A1单缺体。在主要农艺性状方面,EAP74961、05黑初8和Amigo抽穗期与石4185(CK)相近,而CH62444则抽穗较晚,并且3份国外材料植株均较高。这4份突变体材料麸皮中的非水溶性戊聚糖含量相对于水溶性戊聚糖占绝对优势,而胚乳中非水溶性戊聚糖含量与水溶性戊聚糖含量则较为接近,但CH62444、Amigo和05黑初8麸皮中的总戊聚糖含量较高(分别为14.33%,15.20%和12.91%),而EAP74961面粉中的总戊聚糖含量(1.34%)较高。在淀粉组分含量方面,4份突变体材料差异不大。 相似文献
12.
结合卫河自身特点和地理环境建立13项指标体系,运用基于熵权的模糊数学模型对其脆弱性进行评价。结果表明,鹤壁的脆弱性为0.69,焦作为0.55,新乡为0.39,安阳为0.36,濮阳为0.23。最后针对评价结果分析了原因,提出了合理性建议和措施。 相似文献
13.
使用可编程控制器对汽车尾气过滤载体所需要的净化催化剂溶液的工艺过程进行自动化操作,系统由S7-200通过软件和硬件对各参数实现在线检测和控制。经生产现场调试使用,自动控制效果良好,达到预期的目的和要求,用户满意。 相似文献
14.
木本园林植物对土壤重金属的富集及修复效应研究进展 总被引:1,自引:0,他引:1
我国城市土壤重金属污染形势严峻,已经严重威胁到人类健康,因此受到普遍关注.木本园林植物是城市绿化、建设的主体,是城市生态系统中富有生命力的单元.植物能绿化、美化环境但不会进入食物链,可以对重金属污染土壤进行持续修复,已成为城市土壤污染植物修复的重要研究领域.该研究综述了近年来用于重金属污染土壤修复的木本园林植物,对其修复土壤的强化措施进行了探讨.重点阐述了木本园林植物对各种重金属的富集效应,对其耐受性和净化土壤重金属污染的应用潜力进行了分析,并对未来的发展方向进行了展望. 相似文献
15.
16.
CHEN Tian-tian CHENG Fu-liang ZHANG Jian-mei NIE Zhao-jing FANG Dong FAN Mei-na LI Fu-hui CHENG He-gang 《中国畜牧兽医》2015,42(9):2474-2480
The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea. 相似文献
17.
WANG Meng-zhu LIU Yan-feng WANG Wen-qi NIE Cun-xi TANG Shu-zhen ZHANG Wen-ju 《中国畜牧兽医》2015,42(9):2345-2351
This experiment was conducted to evaluate the effect of alfalfa flavonoids (Medicago sativa) on growth performance and serum indexes of sheep.This experiment was performed with hybrid F1 sheep of Suffolk sheep×Small-tailed Han sheep.Twenty-eight sheep with the body weight of (27.02±3.03) kg were randomly divided into 4 groups with 7 replicates in each group and 1 sheep per replicate.Sheep in different groups were fed diets with 0 (group Ⅰ), 0.1% (group Ⅱ), 0.2%(group Ⅲ) and 0.4% (group Ⅳ) alfalfa flavonoids, respectively.The experiment was cosisted of 7-day-preliminary trial and 41-day-formal trial.The results showed as follows:① Dietary supplementation of alfalfa flavonoids had no significant effects on growth performance of sheep(P>0.05).② Dietary supplementation of alfalfa flavonoids had significant effects on the contents of GLB, ASP, BUN, HDL and P in serum (P<0.05).Serum GLB content at the 1st day in group Ⅲ was significantly lower than that in groupsⅠand Ⅱ(P<0.05), serum ASP contents at the 1st and 21th days in group Ⅳ were significantly lower than those in group Ⅰ(P<0.05), serum BUN content at the 1st day in group Ⅳ was significantly lower than that in group Ⅰ(P<0.05), serum HDL content at the 21th day in group Ⅱ was significantly lower than that in groups Ⅰ and Ⅲ (P<0.05), serum P content in group Ⅳ at the 21th day was significantly lower than that in groups Ⅱ and Ⅲ (P<0.05).In conclusion, dietary supplementation of alfalfa flavonoids might modulate lipid metabolism, improve nitrogen utilization, maintain energy metabolic and balance Ca and P contents, and have the potential function for promoting growth and no adverse effect on liver.The results suggested that the appropriate supplemental level of alfalfa flavonoids for sheep was 0.2%. 相似文献
18.
19.
20.
Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events
To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border(RB) and left border(LB) regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head(RB-to-RB) concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events. 相似文献