昭通烤烟生态气候适宜性分析

为探索昭通烤烟生态气候资源分布状况,指导烤烟优化布局,本文利用昭通各区县气象站1980—2004年的气象观测资料,通过与已有优质烟区气象资料的对比分析,考察昭通烤烟种植的气候适宜性;利用合理的烤烟生态气候适宜性指标对昭通烤烟种植区进行重新分区,得出：昭通西南大部地区和东部的部分地区（属北亚热带气候区域）为最适宜烟区,西南部海拔在1800—2000m的坝区和大部山区以及东北部海拔900—1700m的大部山区（属南温带气候区域）为适宜烟区。     

NKX3.1 down-regulates bcl-2 gene expression in prostate cancer PC-3 cells

AIM: To investigate the effect of NKX3.1 on the gene expression of bcl-2 and apoptosis in prostate cancer PC-3 cells. METHODS: The eukaryotic expression plasmid pcDNA3.1- NKX3.1 was transiently transfected into PC-3 cells. RT-PCR and Western blotting were used to detect the effects of NKX3.1 on the expression of bcl-2 gene. Down-regulatory effect of NKX3.1 on bcl-2 gene was detected by EMSA. Flow cytometry and apoptotic body staining were carried out to study the effects of NKX3.1 on apoptosis of PC-3 cells. RESULTS: The mRNA and protein expression of bcl-2 in PC-3 cells was down-regulated by over-expression of NKX3.1. The EMSA result showed that NKX3.1 interacted with the NKX3.1 binding elements in upstream regulatory region of bcl-2 gene. The results of flow cytometry showed that the number of apoptotic PC-3 cells increased by 1.41-fold after NKX3.1 transfection to PC-3 cells. NKX3.1 increased the apoptotic bodies stained by Hoechst 33258 significantly. CONCLUSION: NKX3.1 down-regulates the expression of anti-apoptotic gene bcl-2 and induces the apoptosis of prostate cancer PC-3 cells.     

望天树人工林生长量及土壤理化性质

望天树是我国一级珍稀濒危保护植物,为了更有效地保护这一树种,在桂西南那坡县对29年生望天树人工林的生长量及土壤理化性质进行了研究。结果表明:林分平均胸径、平均树高和平均蓄积量分别为28.49cm、28.30m和440.5m3/hm2,净经济收入为39.65万元/hm2。林地0～100cm土层密度为0.819 8～1.174 3g/cm3,土壤总孔隙度为57.0%～66.3%,土壤通气度为16.8%～25.0%,土壤最大持水量、毛管持水量和最小持水量分别为46.7%～94.2%、34.1%～68.8%和20.8%～42.1%。0～40cm土层pH值为4.64～4.96,土壤有机质含量为29.58～70.70g/kg,土壤全N、全P和全K含量分别为1.15～2.71、1.38～1.56和13.27～15.31g/kg,速效N、P和K分别为157.68～327.42、0.32～1.21和9.93～23.12mg/kg。研究结果表明,29年生望天树人工林的生长量较高,林地土壤疏松、土壤通气性能良好、土壤肥力较高。因此,在类似该地区立地环境进行望天树的引种栽培具有较大的生物生产力。     

项目教学法在生物工程实验课程中的应用——以酿造工艺学实验为例

在总结创新实验方法的基础上,通过对酿造工艺学实验项目内容的调整、实验设计及过程管理的创新、实验方法的改革,将项目教学法应用到酿造工艺学综合实验中,提出了生物工程综合实验的实验内容及项目的设计原则,实验教学实施方法及评价方法,旨在达到激发学生积极主动性及科研兴趣,培养学生组织动手能力、协作能力、综合概括能力、自主分析解决问题等综合能力,并拓展学生思考问题的深度和广度的教学目的。     

不同类型土壤团聚体斥水性及其对溅蚀的影响

为明确土壤的斥水性特征及其对土壤溅蚀的影响,以3种不同类型及利用方式土壤为研究对象,探究了土壤斥水性分布规律及其影响因素,系统分析了土壤斥水性对土壤溅蚀的影响。结果表明:(1)耕地土壤的斥水性最低,表层土壤斥水性高于下层,黄褐土斥水性(13.15sec)高于其他类型土壤,整体上看,斥水性受到土壤机械组成和游离铁、铝氧化物的显著影响(R2-0.40,p0.01);(2)土壤溅蚀过程呈现为倒V形,即一个陡涨陡落的特点,土壤累计溅蚀量随降雨历时变化关系用对数函数可以很好地拟合(R2≥0.90,p0.01);(3)在一定雨强范围内,斥水性对土壤溅蚀有着显著影响,斥水性越大土壤发生溅蚀的程度也越大,对数函数模型也可以很好地表达溅蚀指标随斥水性变化趋势。研究结果能为降雨侵蚀机制研究与侵蚀过程模型提供理论参考依据。     

Kisspeptin/GPR54系统在生殖启动中的作用

青春期的启动始于下丘脑脉冲性GnRH分泌的提高。Kisspeptin/GPR54系统是新发现的能够调节下丘脑GnRH释放的重要信号通路,此系统的发现敲开了生殖启动机理研究的新大门。本文就Kisspeptin/GPR54系统的发现、生物学特性与对生殖启动的调节作用等方面作一综述。     

5种补益类中草药抗诱变作用的对比实验研究

采用小鼠骨髓嗜多染红细胞微核试验、小鼠骨髓细胞染色体畸变试验、鼠伤寒沙门氏菌营养缺陷型回复突变试验、人外周血淋巴细胞姐妹染色单体交换试验、人外周血淋巴细胞微核试验、中国仓鼠卵巢细胞染色体畸变试验,对人参、绞股蓝、黄芪、甘草和女贞子水提物的低、中、高剂量抗诱变性能进行了实验对比研究.结果显示,人参、绞股蓝、黄芪的中、高剂量组,甘草3个剂量组均能降低环磷酰胺(CP)诱发的微核率(p<0.05);绞股蓝、甘草3个剂量组,人参高剂量组,黄芪中、高剂量组对CP诱发的染色体畸变率增高有抑制效应(p<0.05);人参、绞股蓝、甘草3个剂量组,黄芪中、高剂量组均能抑制由2,7-AF诱发的TA98菌株回变菌落数增加(p<0.05);黄芪、女贞子3个剂量组,绞股蓝中、高剂量组均能抑制由NaN3诱发的TA100菌株回变菌落数增高(p<0.05);绞股蓝中、高剂量组,甘草中剂量组能降低由MMC诱发的SCE增高(p<0.05);人参中、高剂量组,绞股蓝、甘草3个剂量组、女贞子低、中剂量组对MMC诱发的MN增高有抑制作用(p<0.05);人参中、高剂量组,绞股蓝、甘草3个剂量组,女贞子低、中剂量组对MMC诱发的染色体畸变有抑制作用(p<0.05).表明绞股蓝、甘草、人参的抗诱变性能良好,作用范围较广;黄芪、女贞子具有一定的抗诱变作用,但有局限性.

Abstract：

To accurately determine the anti-mutation effects of the aqueous extract of five tonic traditional Chinese medicinal herbs [Gynostemma pentaphyllum,Glycyrrhiza uralensis Fisch, Ginseng (Panax ginseng), Astragalus membranaceus and Fructus ligustri] and to determine the dose effect of each. Methods: The world-wide adopted means (micronucleus test of polychromatic erythrocyte in mouse bone marrow cells, chromosomal aberration test of mouse bone marrow cells, Ames test, sister chromatid exchanges test in the lymphocytes of human blood, micronucleus test in the lymphocytes of human blood, chromosomal aberration test of CHO cells) were used to systematically study the anti-mutation effects of the above five traditional Chinese medicines under different doses. Results: Medium to high dose groups for Ginseng,G.pentaphyllum and A.membranaceus and all three dose groups for G.uralensis Fisch de-creased CP-induced micronucleus frequency in mouse bone marrow cells (p<0.05). All three dose groups for G.pentaphyllum and G. uralensis Fisch, the high dose group for Ginseng and the median and high dose groups for G. uralensis Fisch showed inhibition effectiveness to the increase in CP-induced chromo-somal aberration ratio of mouse bone marrow cells (p<0.05). All three dose groups for Ginseng, G.ura-lensis Fisch and G. pentaphyllum restrained the increase in the back matagenesis of TA98 induced by 2,7-AF (p<0.05). For A. membranaceus, however, only the high dose group was effective(p<0.05). All three dose groups for A. membranaceus and F. ligustriand high dose group for G.pentaphyllum inhibi-ted the increase in back matagenesis of T A100 induced by NaN3 (p<0.05). High dose group for G.pen-taphyllum and median dose group for G. pentaphyllum inhibited the increase inf SCE in the lymphocytes of human blood induced by MMC (p<0.05). High dose group for Ginseng, three dose groups for G. pen-taphyllumand low and median groups for F.ligustriinhibited the increase in MN in the lymphocytes of human blood induced by MMC (p<0.05). High dose group for ginseng, three dose groups for G. pen-taphyllum and G. uralensis Fisch, and high dose groups for G.pentaphyllum and G.uralensis Fisch in-hibited chromosomal aberration of CHO cells induced by MMC (p<0.05). Conclusions: G. pentaphyl-lum, G. uralensis Fisch and Ginseng show good anti-mutant activity. Their effective dose ranges are quite wide.A.membranaceus and F.ligustrilucidi showed only limited effectiveness against induced mutation.     

中生菌素高产菌株发酵条件的研究

中生菌素产生菌通过菌种选育，得到４株效价较亲株提高７０％的菌株。通过正交回归实验得到中生菌素高产菌株ＵＶ－７的摇瓶最佳培养条件和最佳发酵配方；明确了通气量和葡萄糖含量是影响最终产素量的主要因素。在６．６Ｌ的全自动发酵罐中优化发酵，明确了ＵＶ－７发酵过程中的代谢规律，证明了发酵代谢的终点与溶解氧和ｐＨ的变化有直接关系。