地下水埋深对夏玉米根冠生长和耗水量的影响

利用排水式蒸渗仪,借助自制的Mariotte瓶装置,通过设置6种地下水埋深控制处理(地下水位埋深分别为0.2、0.4、0.6、0.8、1.01、.2m),探讨了不同地下水埋深对夏玉米的地上部分、根系生物量及耗水量的影响。结果表明,夏玉米株高和冠层叶面积随地下水埋深减小有增加的趋势,但是当地下水埋深减小到一定程度时就会抑制株高、叶面积,地下水埋深0.6m处理株高和叶面积系数(LAI)最大。各土层根系及其总生物量随地下水埋深增大而增大;地上部总生物量与地下部根生物量的比值随地下水埋深的增大呈明显减少的趋势。夏玉米全生育期和各阶段耗水量分别与地下水埋深呈较好的负相关关系,达到极显著水平(P<0.01);地下水补给量占耗水量的比例随地下水埋深的增大而逐步降低。研究结果可为江淮丘陵区夏玉米灌溉制度的制定及农田排水工程的设计提供参考依据。     

高产条件下夏玉米需水量与需水规律研究

依据2009-2010年田间试验资料,利用农田水量平衡方程计算高产条件下夏玉米各生育阶段需水量和全生育期需水量.结果表明,高产条件下,夏玉米全生育期土壤水分维持在田间持水量的80%左右;全生育期需水量为417.30～507.45 mm.各生育阶段需水量分别为:苗期16.80～33.75 mm,占全生育期需水量3.31%...     

不同局部灌溉方式对玉米叶片生理指标的影响

以畦漫灌为对照,对隔行喷灌、隔行沟灌、膜上灌溉和穴灌4种局部灌溉方式下玉米的叶片生理指标变化规律进行了分析。结果表明,局部灌溉对玉米叶片的生理活性产生了一定的影响;膜上灌溉叶片的脯氨酸含量和细胞膜相对透性均不同程度低于畦漫灌,叶绿素、丙二醛含量,细胞间隙CO2浓度、净光合速率、气孔导度、蒸腾速率等指标均有不同程度提高。隔行灌溉、隔行喷灌生理指标接近于畦漫灌,穴灌与畦漫灌相比,生理指标变化与膜上灌溉正好相反。     

半干旱地区果园土壤Cl-迁移积累与环境因子的关系

为揭示环境因子对果园表层土壤氯离子迁移累积的影响,为预防和改良土壤次生盐渍化提供理论指导和相关依据,研究果树生育期表层土壤不同土层氯离子的变化与环境因子（土温、土壤水分、蒸发量、降雨量、水分亏值、气温、空气相对湿度）的相互关系。结果表明：在土壤0~25cm土层的微域范围内,不同土层对氯离子的迁移与累积具有不同的功能。0~5cm土层为氯离子聚集层,10~15cm土层为氯离子传导层。认为10~15cm土层是半干旱地区环境因子对土壤性质直接作用与影响的临界深度。     

越冬栽培稻产量性状相关QTL定位

越冬栽培稻是一类能越过自然冷冬季节并在第2年春季萌芽、正常开花结实、收获稻谷的水稻品种。本文通过对越冬栽培稻产量性状QTL分析,明确产量相关性状的遗传规律,旨在进一步解析越冬栽培稻产量性状的遗传机制,为育种创新利用提供理论依据。以3份越冬栽培稻构建的3个半同胞F2群体为材料。各考察15个产量相关性状,利用Excel 2003、GraphPad Prism 5.0和QTL IciMapping 4.10软件分析数据、绘制遗传图谱、定位QTL和联合分析。结果表明,产量性状表型值在3群体中呈连续正态分布,表现为数量性状遗传。共检测到37个QTL和26对上位性QTL,贡献率分别介于2.32%～36.31%和1.04%～2.05%;检测到9个同时影响2个及以上产量性状(一因多效)QTL标记区间;以联合分析检测到13个产量性状相关QTL,其中4个QTL区间与单群体检测QTL区间重叠;越冬栽培稻产量相关性状QTL以加–显性效应遗传为主、上位性遗传效应为辅。本研究将为越冬栽培稻产量相关基因挖掘及育种创新利用奠定基础。     

青藏高原沙棘属植物种实表型多样性和发芽特性研究

为研究沙棘属植物云南沙棘、江孜沙棘和肋果沙棘各采集点种实表型性状、种子发芽特性及其相关性,通过测定果实的鲜果长、宽、干果长、宽、果实含水量和种子的长度、宽度、长宽比、百粒重、种子含水量等表型性状,以及种子发芽率、发芽势、发芽指数等发芽特性,并比较浸种时间(12,24,48h)和培养温度(20,25,30℃)对种子发芽的影响。结果表明:青藏高原沙棘属3种植物各采集点种实表型性状和种子发芽特性存在显著差异;浸种24h,30℃发芽条件下,青藏高原沙棘属3个种9个采集地点种子发芽率、发芽势和发芽指数均存在显著差异,其中类乌齐县肋果沙棘发芽率(95.68%)最高,白朗县江孜沙棘(86.00%)最低;温度设定在20～30℃之间,3种沙棘植物种子发芽势和发芽指数差异显著;浸种时间12～48h时,沙棘属植物种子发芽率差异显著;浸种24h,在温度25℃和30℃条件下,沙棘属植物种子发芽率、发芽势和发芽指数最高;种子发芽势与种子发芽指数呈极显著正相关,与鲜果宽呈极显著负相关,发芽指数与鲜果宽呈极显著负相关,种子百粒重与鲜果长、种子宽呈极显著正相关。     

Heme oxygenase-1 protects renal function in septic rats via influencing expression of thrombomodulin

AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) on the kidney of septic rats and the influence of HO-1 on the expression of thrombomodulin (TM) in the kidney. METHODS: Sepsis in rats was developed with cecal ligation and puncture (CLP). The septic rats were randomly divided into sham group, CLP group, CLP+HO-1 inducer group and CLP+HO-1 inhibitor group (n=18). The plasma levels of creatinine (Cr), cystatin-C (Cys-C), carboxyhemoglobin (COHb), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and TM, and the changes of prothrombin time (PT) and activated partial thromboplastin time (APTT) in each group were measured. Histopathological examination was performed in the kidney. The expression of TM in the kidney tissue was detected by Western blot. RESULTS: Compared with sham group, significantly elevated plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), shortened PT and APTT (P<0.05), significantly increased microthrombus formation, and lowered TM expression in the kidney (P<0.05) of CLP group were observed. The administration of hemin lowered the plasma levels of Cr, Cys-C, TNF-α, IL-1β and TM (P<0.05), prolonged PT and APTT (P<0.05), attenuated microthrombus formation, and up-regulated the expression of TM in the kidney (P<0.05). In contrast, ZnPP had the opposite effects. CONCLUSION: HO-1 increases the expression of TM in the kidney and exerts anticoagulatory and antiinflammatory effects, thereby improving renal function in the septic rats.     

Effects of autophagy inhibitor bafilomycin A1 on macrophages polarization

AIM:To investigate the effect of bafilomycin A1 (Baf A1) on polarization in mouse macrophages RAW264.7. METHODS:The macrophages RAW264.7 were treated with Baf A1, the concentration of M1/M2 polarization related cytokines were determined by ELISA. The markers of M1/M2 polarization in the macrophages were determined by flow cytometry. The formation of autophagicbody was observed by immunofluorescence. Western blot was used to detect the expression of autophagy related protein levels. RESULTS:The concentration of M1 related proinflammatory cytokine tumor necrosis factor-α (TNF-α) was increased significantly after Baf A1 intervention (P<0.01). However, the concentration of M2 related anti-inflammatory cytokines IL-10 and IL-13 showed no significant difference. The double positive rate of CD197 and HLA-DR (M1 marker) positive cells in Baf A1 treated group were significant higher than that in control group (P<0.05), indicated that Baf A1 induced polarization of macrophage to M1. The results of immunofluoresence showed that the autophagosomes formation was notable increased in Baf A1 group (P<0.05), meanwhile Western blot results showed the expression of autophay related protein LC3-Ⅱ but not P62 was marked up-regulated (P<0.05). For autophagy activator rapamycin (Rapa) treated group, autophagosome formation was also significant increased (P<0.05), but the expression of P62 was notable down-regulated (P<0.05). CONCLUSION:Baf A1 induces polarization of mouse macrophage RAW264.7 to M1, which may be related to the inhibitory effect on the formation of autolysosome.     

盐渍化土壤覆土材料理化性状及其对双孢蘑菇生长的影响

研究山西省当地盐渍化土壤添加草炭土、醋糟、谷糠或高粱壳配制的不同覆土材料(Ⅰ:66.7%草炭土,33.3%盐渍化土壤;Ⅱ:50%草炭土,50%盐渍化土壤;Ⅲ~Ⅴ:每立方米盐渍化土壤分别添加45kg醋糟、谷糠或高粱)的理化性状,并比较不同覆土材料对双孢蘑菇(Agaricus bisporus)生长的影响,以草炭土为对照。从覆土至第一潮菇采收结束,Ⅰ和Ⅱ覆土电导率呈逐渐上升趋势,而Ⅲ~Ⅴ覆土的电导率则一直保持较高水平,为1427~1980μS/cm,第三潮菇采收时,Ⅰ~Ⅴ覆土电导率均显著下降;覆土第6天开始,Ⅰ~Ⅴ覆土的pH明显高于对照,为7.47~8.47;Ⅰ和Ⅱ覆土处理的发菌天数与对照相同,Ⅲ~Ⅴ覆土处理则比对照延长3d;各处理的第一潮菇和第三潮菇的采收时间均为5d,第二潮菇采收时间各处理间略有差异,但总产量均高于对照,其中以Ⅲ覆土处理产量最高,达21.3kg/m~2。试验结果表明,盐渍化土壤添加草炭土、醋糟、谷糠或高粱壳均可作为生产双孢蘑菇的覆土材料。     

山葵黑入病及其防治

对山葵黑入病的症状、病原、侵染循环、发病条件及防治方法等进行了概述.