Effects of Supplementing a Basal Diet of Chloris gayana Hay with One of Three Protein-rich Legume Hays of Cassia rotundifolia,Lablab purpureus and Macroptilium atropurpureum Forage on Some Nutritional Parameters in Goats

Growth and digestibility experiments were conducted on growing East African type goats offered Chloris gayana hay supplemented with one of three high-protein (119–128 g CP/kg DM) legume hays, Cassia rotundifolia (cassia), Lablab purpureus (lablab) or Macroptilium atropurpureum (siratro), and crushed maize to investigate the feed intake, digestibility, growth and urinary excretion of purine derivatives. Goats in the supplemented groups had higher total dry matter and nitrogen intakes and higher N retention and body mass gains than unsupplemented counterparts. The digestibility of dry matter, organic matter and neutral detergent fibre were increased by protein supplementation. Animals on supplemented diets had higher fractional outflow rates of particulate matter from the rumen. The production of protein by ruminal microbes and the efficiency of microbial N production were increased by supplementation. It was concluded that a mixture of low-quality grass hay (61.9 CP/kg DM) and either cassia, lablab or siratro hay, and maize grain can provide a productive balanced diet for growing goats.     

Approaches to modeling intramammary infections in dairy cattle

In this paper, three approaches (Markov processes, discrete-event simulation, and differential equations) to modeling intramammary infections (IMI; focusing on the dynamic changes between uninfected, subclinical, and clinical udder-health states) are described. The objectives were to describe the various approaches to modeling intramammary infections, determine if simulations of the examples of the three approaches yield stable prevalences, and discuss the approaches' limitations. The literature review showed that there is no agreement on the proportion of animals that change health states. The approach of discrete-event simulation modeling included the most cow-level risk factors and udder-health states (hence, was judged to replicated best the dynamics of the infection process) and yielded stable prevalences for all udder-health states. However, there remain parts of the dynamics that need further research. These include the pathogen-specific probabilities and times of occurrence for: regression of clinical IMI to subclinical IMI, flare-up of subclinical IMI to clinical IMI, and incidence of subclinical IMI. Also, the assumption in all current approaches of homogenous mixing is violated because the primary contact structure for contagious pathogens during milking is either between cows through residual infectious milk in the milking machine or within a cow by vacuum fluctuations or teat-cup liner slips. Better contact structures should be incorporated so that the effects of control strategies can be better-estimated. Moreover, the three modeling approaches discussed assumed that all non-infected quarters are susceptible to infection—which might be denied by work in genetic resistance.     

Comparison Between a γ-IFN Assay and Intradermal Tuberculin Test for the Diagnosis of Bovine Tuberculosis in Field Trials in Brazil

Bovine tuberculosis is a major problem in Brazil. The intradermal tuberculin test is the standard test for detection of bovine tuberculosis in Brazil but can lack both sensitivity and specificity. The purpose of this study was to compare a bovine γ-IFN assay with the tuberculin test under field conditions in Brazil. A total of 1632 animals from 13 dairy farms were tested using the single cervical tuberculin test (SCTT). Among those animals, about 15% of each herd, 220 in total, represented a high-risk group and were selected to be tested using the γ-IFN test. Of the 1632 animals tested, 207 presented significant reactions representing 12.7% of the cattle studied. In the selected group the number of animals positive by the γ-IFN assay was 126/220 (57.3%) and the total number of reactive cows on SCTT was 106/220 (48.2%). The real number of infected cattle, following standards, was 120/220 (54.5%). From these results the relative sensitivity rate of γ-IFN test was 100% including the false-positive results and 88.3% for the SCTT — a significant (P < 0.01) difference in favour of the γ-IFN test of 11.7%. The γ-IFN assay also identified some positive animals 60–120 days earlier than the SCTT. In conclusion, we believe that the γ-IFN assay can be used alone or in combination with the SCTT, as a valuable tool for the control of bovine tuberculosis in the Brazilian national herd.     

Comparison of three serologic techniques for detection of antibody to Mycoplasma pulmonis

A comparison of three micro-assay detection systems for naturally occurring and experimentally induced rat antibody to Mycoplasma pulmonis was performed. The micro-enzyme linked immunoabsorbent assay was found superior to counter immunoelectrophoresis or complement fixation techniques when comparing sensitivity, duration of antibody response, or technical requirements. The primary immune response was routinely detected at 3 weeks after antigen exposure when using the enzyme linked or complement fixation assays.     

Prevalence of Giardia in the feces of pups

Fecal specimens were collected from 117 healthy pups (79 privately owned pups and 38 pups from an animal shelter) and analyzed for Giardia. Giardia cysts or trophozoites were found in 35.9% of the dog feces.     

ANGIOGRAPHIC EVALUATION OF BOVINE FOOT ABNORMALITIES

Angiography of normal and diseased feet in cattle was carried out. In osteomyelitis of distal phalanx there was a narrowing of the digital artery with diminished vascular supply to the affected digit. An area of bone necrosis was found to be avascular. With overgrown hooves, narrowing of both the medial and lateral digital arteries with ill-defined laminar vessels was seen. The vascular pattern in cattle with cork screw claws was characterized by a dilated tortuous digital artery and a crowded arterial network resulting in increased vascular supply in the affected claw. Abrupt stricture of common digital artery, a crowded vascular network in the area of the middle phalanx and filling defects of terminal arches were demonstrated in animals with exostosis