Cellular changes in the granulosa layer of the maturing ovarian follicle of the domestic fowl

1. The relationships between length, width and height of the maturing ovarian follicle of the domestic fowl (Gallus domesticus) are shown.

2. During the rapid‐growth phase (yolk deposition) of the oocyte, i.e. an increase in size from about 9 mm diameter to 35 mm diameter, there is about a 15‐fold increase in its surface area. During this time the number of granulosa cells adjacent to the oocyte increases by about 5‐fold.

3. The granulosa cells become flatter during the growth period of the follicle, consequently increasing their surface area adjacent to the oocyte by about 3‐fold.

4. Together the change in shape of the granulosa cells and the increase in their number account for the increase in area of the granulosa layer during follicular growth.

5. Measurement of the DNA content of the granulosa layer indicated a progressive decrease in the cellular content of DNA as the follicle matures.     

A simple caliper technique for the estimation of abdominal fat in live broilers

1. A caliper technique for estimating the amount of abdominal fat in live broilers is described.

2. Sixty‐three male and 47 female broilers sampled at 63 d of age from an unselected control line were used to examine the accuracy of the caliper measure in predicting abdominal fat.

3. Phenotypic correlations between caliper measure and the proportion of abdominal fat were + 0.80, +0.79 and +0.81 in males, females and the heaviest 20% of males respectively.

4. The application of the technique as an indirect means of selecting for decreased abdominal fat in commercial broiler breeding programmes is discussed.     

Shedding of lymphoid leukosis virus in chickens following contact exposure and vaccination

Chickens contact-exposed to lymphoid leukosis virus at various ages up to 32 weeks responded with relatively high rates of infection as determined by the presence of neutralizing antibody. Virus shedding as determined by cloacal swab and albumen testing occurred in 7 of 8 groups of such chickens, but the incidence was 10% or less and sporadic. Vaccination of chickens immediately before exposure with a low pathogenicity virus of subgroup A at 8 weeks of age did not eliminate subsequent shedding.