全文获取类型
收费全文 | 273篇 |
免费 | 18篇 |
国内免费 | 1篇 |
专业分类
林业 | 4篇 |
农学 | 9篇 |
8篇 | |
综合类 | 47篇 |
农作物 | 7篇 |
水产渔业 | 33篇 |
畜牧兽医 | 160篇 |
园艺 | 4篇 |
植物保护 | 20篇 |
出版年
2023年 | 2篇 |
2021年 | 3篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 15篇 |
2012年 | 4篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 8篇 |
2008年 | 10篇 |
2007年 | 8篇 |
2006年 | 8篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 11篇 |
2001年 | 12篇 |
2000年 | 12篇 |
1998年 | 3篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 11篇 |
1994年 | 4篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1977年 | 3篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 6篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1967年 | 2篇 |
排序方式: 共有292条查询结果,搜索用时 343 毫秒
131.
SUMMARY The milk production of cows after induced parturition was compared with that of non-induced cows with similar calving dates, in 37 winter-calving, pasture-fed, commercial dairy herds in south-western Victoria. Parturition was induced when most cows were between 27 and 35 weeks of pregnancy. Milk production was compared for the lactation that commenced immediately after induction. The percentage of cows whose lactations were terminated less than 120 days after calving was increased by induction. Mean percentages for untreated and induced groups were 3.0% and 5.4%, respectively . For cows with lactations of at least 120 days duration, lactation length was not affected by the prior induction of parturition. However, milk production during the subsequent lactation was 9.0% less in induced groups. Fat and protein concentrations were not substantially affected. The reduction in milk production was not affected by stage of pregnancy when cows were induced, but tended to be greater in herds that delayed milking of induced cows . 相似文献
132.
Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I 总被引:43,自引:0,他引:43
M G Murray J Bradley X F Yang E Wimmer E G Moss V R Racaniello 《Science (New York, N.Y.)》1988,241(4862):213-215
The mouse-adapted strain of poliovirus type 2 (Lansing) induces fatal poliomyelitis in mice after intracerebral inoculation, whereas mice inoculated with poliovirus type 1 (Mahoney) show no signs of disease. Previous work indicated that the adaptation to mouse virulence is associated with the viral capsid proteins and that mutations in neutralization antigenic site I of poliovirus reduce neurovirulence of the Lansing strain in mice. The role of antigenic site I in mouse neurovirulence was further explored by constructing an antigenic hybrid virus. Six amino acids in antigenic site I of the Mahoney strain were replaced with a sequence specific for the Lansing strain by using a mutagenesis cartridge. The hybrid virus was neutralized by polyclonal antisera elicited by the type 1 and type 2 strains of poliovirus and by neutralizing monoclonal antibodies directed against antigenic site I of type 2 virus. The hybrid virus induced paralytic disease in mice, an observation demonstrating that a short sequence of amino acids in antigenic site I is an important determinant of poliovirus host range. Antigenic site I may be involved in attachment of poliovirus to cells of the mouse central nervous system. 相似文献
133.
Shemil P. Macelline Leon R. McQuade Bernie V. Mclnerney Amy F. Moss Peter H. Selle Sonia Y. Liu 《动物营养(英文)》2020,6(4):521-528
This study determined the variations in protein digestibilities and digestion rates in broiler chickens offered diets containing 7 different meat and bone meals (MBM). A total of 252 male Ross 308 broiler chickens were offered 7 atypical diets largely based on maize and MBM from 24 to 28 d post-hatch. Each experimental diet was offered to 6 replicates with 6 birds per replicate cage. Excreta were collected in their entirety from 25 to 27 d post-hatch and on 28 d post-hatch. Digesta samples were collected from the proximal jejunum, distal jejunum, proximal ileum and distal ileum. Apparent digestibilities of protein were determined in each segment and apparent digestibilities of amino acids were measured in the distal ileum. There were significant differences in apparent protein digestibility coefficients in the proximal jejunum (P = 0.006), where broiler chickens offered the high ash beef meal (diet 7) generated the lowest protein digestibility in the proximal jejunum (0.318). Similarly, there were significant differences in apparent digestibility coefficients in the distal jejunum (P < 0.001) and distal ileum (P < 0.001) but not in the proximal ileum. More pronounced differences were found in the disappearance rate of protein and there were significant differences in all 4 segments of the small intestine (P < 0.001). Broiler chickens offered the high ash beef meal had the lowest protein disappearance rate (P < 0.001). No difference was observed in the predicted protein digestion rate (P = 0.486) but chickens offered the high ash beef meal had the lowest potential digestible protein (0.662, P = 0.034) whereas the highest potential digestible protein (0.739) was detected in diet 5 (containing a beef meal). This study contributed to the establishment of a preliminary database to include digestion rates of starch and protein into practical diet formulation. 相似文献
134.
Kidd TJ Gibson JS Moss S Greer RM Cobbold RN Wright JD Ramsay KA Grimwood K Bell SC 《Veterinary microbiology》2011,149(3-4):508-512
Pseudomonas aeruginosa is associated with infectious endometritis in horses. Although infectious endometritis is often considered a venereal infection, there is relatively limited genotypic-based evidence to support this mode of transmission. The study sought to determine the relatedness between genital P. aeruginosa isolates collected from a limited geographical region using molecular strain typing. Enterobacterial repetitive intergenic consensus PCR typing was performed on 93 isolates collected between 2005 and 2009 from 2058 thoroughbred horses (including 18 stallions) at 66 studs. While P. aeruginosa was not detected in the stallions, 53/93 (57%) mares harbouring P. aeruginosa had clonally related strains, which included a single dominant genotype detected in 42 (45%) mares from 13 different studs. These novel findings suggest that most equine genital P. aeruginosa infections in this region may have been acquired from mechanisms other than direct horse to horse transmission. Instead, other potential acquisition pathways, as well as strain specific adaptation to the equine genital tract, should be investigated. 相似文献
135.
T-lymphocyte priming and protection against Friend leukemia by vaccinia-retrovirus env gene recombinant 总被引:25,自引:0,他引:25
P L Earl B Moss R P Morrison K Wehrly J Nishio B Chesebro 《Science (New York, N.Y.)》1986,234(4777):728-731
The current prevalence of the acquired immune deficiency syndrome in humans has provoked renewed interest in methods of protective immunization against retrovirus-induced diseases. In this study, a vaccinia-retrovirus recombinant vector was constructed to study mechanisms of immune protection against Friend virus leukemia in mice. The envelope (env) gene from Friend murine leukemia virus (F-MuLV) was inserted into the genome of a vaccinia virus expression vector. Infected cells synthesized gp85, the glycosylated primary product of the env gene. Processing to gp70 and p15E, and cell surface localization, were similar to that occurring in cells infected with F-MuLV. Mice inoculated with live recombinant vaccinia virus had an envelope-specific T-cell proliferative response and, after challenge with Friend virus complex, developed neutralizing antibody and cytotoxic T cells (CTL) and were protected against leukemia. In contrast, unimmunized and control groups developed a delayed neutralizing antibody response, but no detectable CTL, and succumbed to leukemia. Genes of the major histocompatibility complex influenced protection induced by the vaccinia recombinant but not that induced by attenuated N-tropic Friend virus. 相似文献
136.
Vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D prevents latent herpes in mice 总被引:28,自引:0,他引:28
K J Cremer M Mackett C Wohlenberg A L Notkins B Moss 《Science (New York, N.Y.)》1985,228(4700):737-740
In humans, herpes simplex virus causes a primary infection and then often a latent ganglionic infection that persists for life. Because these latent infections can recur periodically, vaccines are needed that can protect against both primary and latent herpes simplex infections. Infectious vaccinia virus recombinants that contain the herpes simplex virus type 1 (HSV-1) glycoprotein D gene under control of defined early or late vaccinia virus promoters were constructed. Tissue culture cells infected with these recombinant viruses synthesized a glycosylated protein that had the same mass (60,000 daltons) as the glycoprotein D produced by HSV-1. Immunization of mice with one of these recombinant viruses by intradermal, subcutaneous, or intraperitoneal routes resulted in the production of antibodies that neutralized HSV-1 and protected the mice against subsequent lethal challenge with HSV-1 or HSV-2. Immunization with the recombinant virus also protected the majority of the mice against the development of a latent HSV-1 infection of the trigeminal ganglia. This is the first demonstration that a genetically engineered vaccine can prevent the development of latency. 相似文献
137.
Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus 总被引:15,自引:0,他引:15
G L Smith G N Godson V Nussenzweig R S Nussenzweig J Barnwell B Moss 《Science (New York, N.Y.)》1984,224(4647):397-399
The gene coding for the circumsporozoite antigen of the malaria parasite Plasmodium knowlesi was inserted into the vaccinia virus genome under the control of a defined vaccinia virus promoter. Cells infected with the recombinant virus synthesized polypeptides of 53,000 to 56,000 daltons that reacted with monoclonal antibody against the repeating epitope of the malaria protein. Furthermore, rabbits vaccinated with the recombinant virus produced antibodies that bound specifically to sporozoites. These data provide evidence for expression of a cloned malaria gene in mammalian cells and illustrate the potential of vaccinia virus recombinants as live malaria vaccines. 相似文献
138.
A single amino acid interchange yields reciprocal CTL specificities for HIV-1 gp160 总被引:23,自引:0,他引:23
H Takahashi S Merli S D Putney R Houghten B Moss R N Germain J A Berzofsky 《Science (New York, N.Y.)》1989,246(4926):118-121
For the IIIB isolate of human immunodeficiency virus type-1 (HIV-1), the immunodominant determinant of the envelope protein gp160 for cytotoxic T lymphocytes (CTLs) of H-2d mice is in a region of high sequence variability among HIV-1 isolates. The general requirements for CTL recognition of peptide antigens and the relation of recognition requirements to the natural variation in sequence of the HIV were investigated. For this purpose, a CTL line specific for the homologous segment of the envelope from the MN isolate of HIV-1 and restricted by the same class I major histocompatibility (MHC) molecule (Dd) as the IIIB-specific CTLs was raised from mice immunized with MN-env-recombinant vaccinia virus. The IIIB-specific and MN-specific CTLs were completely non-cross-reactive. Reciprocal exchange of a single amino acid between the two peptide sequences, which differed in 6 of 15 residues, led to a complete reversal of the specificity of the peptides in sensitizing targets, such that the IIIB-specific CTLs lysed targets exposed to the singly substituted MN peptide and vice versa. These data indicate the importance of single residues in defining peptide epitopic specificity and have implications for both the effect of immune pressure on selection of viral mutants and the design of effective vaccines. 相似文献
139.
M F Good L H Miller S Kumar I A Quakyi D Keister J H Adams B Moss J A Berzofsky R Carter 《Science (New York, N.Y.)》1988,242(4878):574-577
Current vaccine development strategies for malaria depend on widespread immunological responsiveness to candidate antigens such as the zygote surface antigens and the sporozoite coat protein, the circumsporozoite (CS) protein. Since immunological responsiveness is controlled mainly by genes mapping within the major histocompatibility complex (MHC), the humoral immune response to the zygote surface antigens and the cytotoxic T lymphocyte (CTL) response to the CS protein were examined in MHC-disparate congenic mouse strains. Only two of six strains responded to the 230-kilodalton zygote surface antigen and another two strains responded to the 48/45-kilodalton surface antigen. From two mouse strains, expressing between them five different class I MHC molecules, there was recognition of only a single CTL epitope from the CS protein, which was from a polymorphic segment of the molecule. The restricted CTL response to this protein parallels the restricted antibody response to this protein observed in humans and mice. These findings suggest that subunit malaria vaccines now being developed may be ineffective. 相似文献
140.
Amara RR Villinger F Altman JD Lydy SL O'Neil SP Staprans SI Montefiori DC Xu Y Herndon JG Wyatt LS Candido MA Kozyr NL Earl PL Smith JM Ma HL Grimm BD Hulsey ML Miller J McClure HM McNicholl JM Moss B Robinson HL 《Science (New York, N.Y.)》2001,292(5514):69-74
Heterologous prime/boost regimens have the potential for raising high levels of immune responses. Here we report that DNA priming followed by a recombinant modified vaccinia Ankara (rMVA) booster controlled a highly pathogenic immunodeficiency virus challenge in a rhesus macaque model. Both the DNA and rMVA components of the vaccine expressed multiple immunodeficiency virus proteins. Two DNA inoculations at 0 and 8 weeks and a single rMVA booster at 24 weeks effectively controlled an intrarectal challenge administered 7 months after the booster. These findings provide hope that a relatively simple multiprotein DNA/MVA vaccine can help to control the acquired immune deficiency syndrome epidemic. 相似文献