Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

超抗原及其在兽医科学上的应用

超抗原以极低的浓度就能引起机体免疫细胞、细胞因子和抗体的巨大变化,引起机体一系列病理生理过程。鉴于超抗原的这种特性,医学上已成功将其用于癌症和病毒病的辅助治疗。在兽医科学上,超抗原有望在兽医病理学、兽医药理学、兽医临床治疗和兽医预防方面得到广泛的应用。     

Role of neutrophil elastase and myeloperoxidase in asthma of rats

AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO.     

中药蛇床子对钉螺浸杀作用及机制的研究

中药蛇床子有杀虫作用,用蛇床子进行钉螺浸杀试验,研究蛇床子的杀螺效果。将蛇床子水提取液和乙醇提取液配制成不同浓度作浸杀钉螺试验,观察钉螺死亡率及蛇床子对钉螺软组织中总蛋白含量和糖原含量的影响,并检测蛇床子提取液对鱼类的毒害作用。结果表明蛇床子有较强的杀螺作用,而且对鱼类的毒性不显著。     

真姬菇培养时间与栽培料失重、含水量和产量的关系

研究了菌丝生长和后熟阶段真姬菇培养料在不同培养时间的失重和含水量变化,及其不同后熟时间对真姬菇产量的影响。结果表明,培养80-90d、培养料含水量达到73％～74％时进行出菇管理获得的真姬菇单产最高,平均为每瓶132．6-146．3g之间。真姬菇栽培可以测定培养基质的水分含量作为菌丝生理成熟指标,确立真姬菇的适宜后熟时间。     

免耕稻油轮作模式探讨

稻油轮作是保障我国粮油安全的重要耕作制度.从农业节肥减药、生态高效、机械化轻简栽培等视角出发,提出了适合湖南地区的免耕稻油轮作模式,阐述了关键技术,分析其可行性,并对其可持续发展进行展望.