Windrow Composting of Waste Paint Sludge Containing Melamine Resins

ABSTRACT

Melamine resins (MR), widely used in various industries, are thermosetting plastics that have hard and durable properties. These resins can be introduced into the environment as components of waste products or via industrial effluents. It is important to seek environmentally friendly means to recycle, remove, or degrade melamine resins before they are released to the environment. Waste paint sludge containing MR has been shown to be efficiently composted under bench-scale conditions. In this study, the impacts of C/N ratios on windrow composting of waste paint sludge containing MR were evaluated. As composting proceeded, temperature, concentrations of MR and melamine degradation products (i.e., ammeline, ammelide, and cyanuric acid) were determined. After 98 days of composting, 87.5, 83.3, 80.9, and 70.1% of the initial MR were degraded under the treatments with C/N ratios of 30, 33, 35, and 37, respectively. Degradation of MR was enhanced by relatively low C/N ratios. Significant amounts of plant nutrients were found in the final composts, while heavy metals were well below values considered to be of concern regarding surface water quality or crop production. We conclude that adjusting C/N ratio to approximately 30 in the initial mixtures with oat and hay straws can increase the degradation of MR and its derivatives during windrow composting of waste paint sludge containing MR and produce a good quality compost product.     

Triacylglycerol and fatty acid compositions of French virgin olive oils. Characterization by chemometrics

There is no data concerning the fatty acid and triacylglycerol composition of French virgin olive oil. Thus, these compositions were determined using 564 samples coming from four olive harvests (1998-1999 to 2000-2001). Among these 564 samples, 372 came from the four main French cultivars (Aglandau, Cailletier, Picholine, and Salonenque) and from both of the oldest French protected designations of origin: "Nyons" (cv. Tanche) and "Vallée des Baux". The fatty acid compositions took the different isomeric monounsaturated fatty acids (C16:1 and C18:1) into account. The eicosenoic acid is gondoic acid (20:1n-9) and was determined by dimethyl disulfide adduct using GC/MS. The use of propionitrile as a mobile phase for the HPLC analysis of the triacylglycerols led to better resolutions between triacylglycerols than those resolutions obtained with the mix of solvents recommended by the normalized method (acetone/acetonitrile). Of the samples, 88 had a 9-heptadecenoic acid level (17:1n-8) higher than 0.3% and 33 had a linolenic acid level higher than 0.9%, which are maximal values accepted by the International Olive Oil Council and the European Union. A linear discriminant analysis was carried out on 372 samples with the SAS system and particularly with STEPISC and CANDISC procedures. Variables (n = 37) representing the different fatty acids, the sum of saturated, monounsaturated, and polyunsaturated fatty acids, squalene, and triacylglycerols were used, thus allowing us to classify samples into six groups defined with 100% of well classified samples. These results constitute an original data bank that can be used to identify the origin of virgin olive oils.     

Iron modulates the replication of virulent Mycobacterium bovis in resting and activated bovine and possum macrophages

Bovine and possum macrophages were infected in vitro with a virulent strain of Mycobacterium bovis, and mycobacterial replication was measured in the infected macrophages cultured under a variety of conditions. Virulent M. bovis replicated substantially in alveolar possum macrophages as well as in bovine blood monocyte-derived macrophages. Addition of recombinant bovine interferon-gamma (IFN-gamma) with low concentrations of lipopolysaccharide (LPS) rendered bovine macrophages significantly more resistant to M. bovis replication. Disruption of iron levels in infected macrophages by addition of apotransferrin or bovine lactoferrin blocked replication of M. bovis in both bovine and possum macrophages. On the other hand, addition of exogenous iron, either in the form of iron citrate or iron-saturated transferrin, rendered macrophages of both species much more permissive for the replication of M. bovis. The impact of iron deprivation/loading on the mycobacteriostatic activity of cells was independent of nitric-oxide release, as well as independent of the generation of oxygen radical species in both possum and bovine macrophages. Exogenous iron was shown to reverse the ability of IFN-gamma/LPS pulsed bovine macrophages to restrict M. bovis replication. When autologous possum lymphocytes from animals vaccinated with M. bovis strain BCG were added to infected macrophages, they rendered the macrophages less permissive for virulent M. bovis replication. Loading the cells with iron prior to this macrophage-lymphocyte interaction, reversed this immune effect induced by sensitized cells. We conclude that, in two important animal species, intracellular iron level plays an important role in M. bovis replication in macrophages, irrespective of their activation status.