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51.
Summary The axial forces generated by the earthworm Aporrectodea rosea were measured by directing the earthworm to tunnel into soil discs mounted on an electronic balance connected to a datalogger. The area over which the force acted was estimated from the size of the hole created by the tunnelling. The maximum force recorded by an individual worm was 0.760 N and the mean maximum value was 0.465 N. The maximum pressures recorded was 116.5 kPa, while the mean maximum pressure was 72.8 kPa. These axial pressures are approximately one-tenth of the maximum axial pressure recorded for plant roots. The statistical distribution of activity as the worms tunnelled through the soil discs was analysed. Pressures recorded were well in excess of physiologically measured pressures and the generation of forces within muscles to achieve these pressures was calculated by some assumptions. It was estimated that there is a maximum tension in the circular muscles of 0.15 N per segment of the worm. 相似文献
52.
Hannah R. Leventhal Anna M. Hassebroek Francisco Carvallo Harold C. McKenzie 《Journal of veterinary diagnostic investigation》2021,33(6):1123
A 14-y-old pony mare was referred after 30-d duration of intermittent pyrexia, anorexia, weight loss, and change in manure consistency. Physical examination revealed a palpable but reducible ventral abdominal mass. Transabdominal ultrasonography revealed multiple distended, hypomotile, and thickened small intestinal loops in close approximation with numerous, well-defined, hyperechoic masses. There was a large amount of echogenic peritoneal fluid; abdominocentesis revealed a neutrophilic and macrophagic inflammatory exudate, and a mixed bacterial population was cultured. Given the poor prognosis, the mare was euthanized. The autopsy findings included a large abdominal abscess, serosanguineous peritoneal fluid with fibrin strands, and ~50 outpouches communicating with the lumen and extending from the anti-mesenteric aspect of the duodenum, jejunum, and ileum. These structures were classified as pseudodiverticula based on the histologic absence of the tunica muscularis layer of the intestinal wall. Pseudodiverticula should be included as a differential etiology in horses when clinical signs consistent with colic, diarrhea, or weight loss are recognized and, when on examination, one or more organized masses are palpated or visualized on transabdominal ultrasound, as well as visualization of small intestinal loops with thickened walls. 相似文献
53.
Rheological Properties of Full-Formula Doughs Derived from Near-Isogenic 1BL/1RS Translocation Lines
Four pairs of near-isogenic wheat lines, with and without the 1BL/1RS translocation, and differing at the Glu-1 loci (coding for high molecular weight [HMW] glutenin subunits) were evaluated for their dough mixing properties, dough stickiness, and baking performance. In all 1BL/1RS translocation lines, weakening of the dough consistency occurred within 2 min past peak time. The full-formula dough from every 1BL/1RS translocation line exhibited poor dough mixing characteristics and increased stickiness compared to the corresponding wheat control. The HMW glutenin subunits coded by the Glu-A1 locus had no apparent effect on mixing properties, but did have a slight effect on the dough stickiness at two of the four stages of dough mixing. Glu-B1 and Glu-D1 loci encoded glutenin subunits produced significant changes in dough mixing properties and dough stickiness, respectively. With respect to baking performance, there was no significant difference between loaf volumes of 1BL/1RS versus control wheats for three of four near-isogenic pairs. Within the 1RS-group, the translocation lines containing HMW glutenin subunits 5+10 produced bread with greater loaf volumes than the pairs containing its allelic counterpart 2+12. Loaf volume was not influenced by the subunits associated with the Glu-B1 loci. In general, the breads baked from 1BL/1RS translocation lines had a relatively poor crumb and crust quality and contained larger gas cells than the wheat controls. In comparing isogenic pairs, the magnitude of the difference in loaf volume between the control wheat and the corresponding 1BL/1RS translocation line was greater in the pair unique for HMW subunits 5+10; the difference was primarily due to the stronger mixing properties of the wheat control. 相似文献
54.
Soil moisture and water use by pastures and silvopastures in a sub-humid temperate climate in New Zealand 总被引:1,自引:1,他引:0
Soil moisture content from 0 to 2 m depth was monitored under 2–6 year old radiata pine (Pinus radiata) with three understoreys of bare ground, lucerne (Medicago sativa) and ryegrass/clover (Lolium perenne/Trifolium spp.) and under adjacent open-grown lucerne and ryegrass/clover pastures. By the fifth year soil moisture depletion/recharge
pattern under the trees alone was similar to that under open pasture and under trees with pasture understoreys. Maximum plant
available moisture storage was 207–223 mm in the top meter of this Templeton silt loam soil but only 69–104 mm at 1–2 m depth
where coarse textures often predominated. Lucerne reduced soil moisture content (SMC) to lower levels during drier summers
and extracted more water from 1 to 2 m depth than ryegrass/clover. Evapotranspiration (ET) during early summer when soil moisture
was high was close to the Penman potential evapotranspiration (E
p
), but the difference increased when SMC in the top meter dropped below 200 mm. The silvopasture treatments had higher ET
in winter than pasture alone but this was still less than E
p
. Soil moisture deficits (SMD) at the end of each summer were sufficiently large to require slightly higher than normal winter
rainfall and ET < E
p
to recharge the soil to field capacity before the next summer. The soil moisture results, taken together with root and growth
data, suggest that trees and understorey pastures are complementary in the first three or four growing seasons but this balance
subsequently declines in favor of the pine trees. Management options, to extend the period that understorey pastures are productive,
include reducing tree stockings, more vigorous pruning, using competitive understoreys and changing from pines to deciduous
trees. Research on new silvopastoral combinations is suggested. 相似文献
55.
Daniel J Marshall Alice Hayward Dominic Eales Michael Imelfort Jiri Stiller Paul J Berkman Terry Clark Megan McKenzie Kaitao Lai Chris Duran Jacqueline Batley David Edwards 《Plant methods》2010,6(1):1-6
Background
The introduction of second generation sequencing technology has enabled the cost effective sequencing of genomes and the identification of large numbers of genes and gene promoters. However, the assembly of DNA sequences to create a representation of the complete genome sequence remains costly, especially for the larger and more complex plant genomes.Results
We have developed an online database, TAGdb, that enables researchers to identify paired read sequences that share identity with a submitted query sequence. These tags can be used to design oligonucleotide primers for the PCR amplification of the region in the target genome.Conclusions
The ability to produce large numbers of paired read genome tags using second generation sequencing provides a cost effective method for the identification of genes and promoters in large, complex or orphan species without the need for whole genome assembly. 相似文献56.
Rachel P. Atherton Martin O. Furr Harold C. McKenzie Anne M. Desrochers 《Journal of Equine Veterinary Science》2011,31(1):19-25
Colitis in the adult horse is a life-threatening clinical condition that can be caused by any of several enteric pathogens. This study was conducted to determine whether treating horses with plasma obtained from donors that were hyperimmunized against the common equine diarrheal pathogens Clostridium difficile, Clostridium perfringens, and Salmonella sp shortens the duration of diarrhea in acute colitis. To evaluate the efficacy of plasma treatment, 42 horses with acute onset of diarrhea were studied. Horses were enrolled if they were of age >1 year, duration of diarrhea at presentation was <72 hours, and they had not received equine plasma within the last 3 months. In addition, the serum cortisol concentrations of horses with acute diarrhea were studied.Horses were randomized to receive hyperimmunized plasma, control plasma (collected from nonimmunized horses), or no plasma therapy. Clinical parameters and fecal consistency were observed until resolution, discharge, or death, and complete blood counts (CBCs) and biochemical profiles were collected throughout the study. A total of 38 horses completed the study. The mean duration of diarrhea was 40.7 ± 9.8 (mean ± SEM) hours, 119.2 ± 56.1 hours, and 72.0 ± 24.5 hours for the hyperimmunized plasma, normal plasma (NP), and control groups, respectively. Using survival analysis techniques, this difference did not achieve statistical significance (P = .374). Serum cortisol was found to be increased in all horses at presentation and to decrease with time in all treatment groups. There was no difference in cortisol concentrations between the three treatment groups studied (P = .237). 相似文献
57.
Brummell DA Watson LM Pathirana R Joyce NI West PJ Hunter DA McKenzie MJ 《Journal of agricultural and food chemistry》2011,59(20):10987-10994
Methylselenocysteine (MeSeCys) is an amino acid derivative that possesses potent anticancer activity in animals. Plants that can tolerate growth on soils with high Se content, known as Se hyperaccumulators, do so by converting inorganic Se to MeSeCys by the enzyme selenocysteine methyltransferase (SMT). A cDNA encoding the SMT from a Se hyperaccumulator was overexpressed in tomato (Solanum lycopersicum). Transgenic plants were provided with selenite or selenate to the roots during fruit development, and liquid chromatography-mass spectrometry was used to show that MeSeCys accumulated in the fruit but not in the leaves. Depending on the transgenic line and Se treatment, up to 16% of the total Se in the fruit was present as MeSeCys. MeSeCys was produced more effectively from selenite on a percentage conversion basis, but greater accumulation of MeSeCys could be achieved from selenate due to its better translocation from the roots. MeSeCys was heat stable and survived processing of the fruit to tomato juice. 相似文献
58.
Fletcher MT Brock IJ Reichmann KG McKenzie RA Blaney BJ 《Journal of agricultural and food chemistry》2011,59(9):5133-5138
Austral bracken Pteridium esculentum contains three unstable norsesquiterpene glycosides: ptaquiloside, ptesculentoside, and caudatoside, in variable proportions. The concentration of each of the glycosides was determined in this study as their respective degradation products, pterosin B, pterosin G and pterosin A, by HPLC-UV analysis. Samples of P. esculentum collected from six sites in eastern Australia contained up to 17 mg of total glycoside/g DW, with both ptaquiloside and ptesculentoside present as major components accompanied by smaller amounts of caudatoside. Ratios of ptaquiloside to ptesculentoside varied from 1:3 to 4:3, but in all Australian samples ptesculentoside was a significant component. This profile differed substantially from that of P. esculentum from New Zealand, which contained only small amounts of both ptesculentoside and caudatoside, with ptaquiloside as the dominant component. A similar profile with ptaquiloside as the dominant glycoside was obtained for Pteridium aquilinum subsp. wightianum (previously P. revolutum ) from northern Queensland and also P. aquilinum from European sources. Ptesculentoside has chemical reactivity similar to that of ptaquiloside and presumably biological activity similar to that of this potent carcinogen. The presence of this additional reactive glycoside in Australian P. esculentum implies greater toxicity for consuming animals than previously estimated from ptaquiloside content alone. 相似文献
59.
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