Evaluation of vaginal implant transmitters in elk (Cervus elaphus nelsoni).

The effects of vaginal implant transmitters for tissue damage after 11 wk in 13 captive adult elk (Cervus elaphus nelsoni) and subsequent reproductive performance in 38 free-ranging elk were evaluated. Vaginal implant transmitters are designed to be shed at parturition and are used to locate birth sites of wild ungulates; however, potential adverse effects of these transmitters on tissues associated with the vaginal walls or subsequent reproductive performance have not been assessed. Vaginal implant transmitters consist of a transmitter encased in inert acrylic with an antenna trailing out the distal end and wings at the proximal end to hold the transmitter in place. Using a laparoscope on sedated captive elk, necrosis or measurable differences in tissue trauma between designs with wing spans of 80 versus 150 mm over an 11-wk trial were not observed. After the captive elk trial, vaginal implant transmitters with 80-mm wings were placed into 38 pregnant wild elk, and 31 live births were documented. Fates of seven calves were not determined, because their transmitters were not shed at the birth site. We recaptured 36 of these cow elk again in fall 2003 or spring 2004, and 32 were pregnant. This study was unable to document any short- or long-term effects of vaginal implant transmitters on reproductive performance of cow elk in captive and free-range environments.     

Toxicologic studies of a conjugate of asparaginase and polyethylene glycol in mice, rats, and dogs

A conjugate of asparaginase and monomethoxypolyethylene glycol was evaluated in acute, subacute, and subchronic toxicologic studies in mice, rats, and dogs. The drug induced low-grade toxicosis. The appearance and behavior of rats and dogs were not affected by the treatment. Only large doses produced inactivity, loss of appetite, and loss of weight. The LD50 could not be established. The drug retarded slightly body weight gains in dogs and female rats and produced mild anemia in 30% of the female rats. Urinalysis and blood chemical determinations in rats and dogs were generally not affected by the treatment. Monomethoxypolyethylene glycol-asparaginase was detectable in the plasma of mice 13 days after IV, intraperitoneal, or IM administration, and in dogs for 3 to 4 weeks.     

Determination of noncovalent docking by infrared spectroscopy of cold gas-phase complexes

Multidentate, noncovalent interactions between small molecules and biopolymer fragments are central to processes ranging from drug action to selective catalysis. We present a versatile and sensitive spectroscopic probe of functional groups engaged in hydrogen bonding in such contexts. This involves measurement of the frequency changes in specific covalent bonds upon complex formation, information drawn from otherwise transient complexes that have been extracted from solution and conformationally frozen near 10 kelvin in gas-phase clusters. Resonances closely associated with individual oscillators are easily identified through site-specific isotopic labeling, as demonstrated by application of the method to an archetypal system involving a synthetic tripeptide known to bind biaryl substrates through tailored hydrogen bonding to catalyze their asymmetric bromination. With such data, calculations readily converge on the plausible operative structures in otherwise computationally prohibitive, high-dimensionality landscapes.     

Quantum deconstruction of the infrared spectrum of CH5+

We present two quantum calculations of the infrared spectrum of protonated methane (CH5+) using full-dimensional, ab initio-based potential energy and dipole moment surfaces. The calculated spectra compare well with a low-resolution experimental spectrum except below 1000 cm(-1), where the experimental spectrum shows no absorption. The present calculations find substantial absorption features below 1000 cm(-1), in qualitative agreement with earlier classical calculations of the spectrum. The major spectral bands are analyzed in terms of the molecular motions. Of particular interest is an intense feature at 200 cm(-1), which is due to an isomerization mode that connects two equivalent minima. Very recent high-resolution jet-cooled spectra in the CH stretch region (2825 to 3050 cm(-1)) are also reported, and assignments of the band origins are made, based on the present quantum calculations.     

Detection of silica-rich deposits on Mars

Mineral deposits on the martian surface can elucidate ancient environmental conditions on the planet. Opaline silica deposits (as much as 91 weight percent SiO2) have been found in association with volcanic materials by the Mars rover Spirit. The deposits are present both as light-toned soils and as bedrock. We interpret these materials to have formed under hydrothermal conditions and therefore to be strong indicators of a former aqueous environment. This discovery is important for understanding the past habitability of Mars because hydrothermal environments on Earth support thriving microbial ecosystems.     

The major-element composition of Mercury's surface from MESSENGER X-ray spectrometry

X-ray fluorescence spectra obtained by the MESSENGER spacecraft orbiting Mercury indicate that the planet's surface differs in composition from those of other terrestrial planets. Relatively high Mg/Si and low Al/Si and Ca/Si ratios rule out a lunarlike feldspar-rich crust. The sulfur abundance is at least 10 times higher than that of the silicate portion of Earth or the Moon, and this observation, together with a low surface Fe abundance, supports the view that Mercury formed from highly reduced precursor materials, perhaps akin to enstatite chondrite meteorites or anhydrous cometary dust particles. Low Fe and Ti abundances do not support the proposal that opaque oxides of these elements contribute substantially to Mercury's low and variable surface reflectance.     

Imaging the microscopic structure of shear thinning and thickening colloidal suspensions

The viscosity of colloidal suspensions varies with shear rate, an important effect encountered in many natural and industrial processes. Although this non-Newtonian behavior is believed to arise from the arrangement of suspended particles and their mutual interactions, microscopic particle dynamics are difficult to measure. By combining fast confocal microscopy with simultaneous force measurements, we systematically investigate a suspension's structure as it transitions through regimes of different flow signatures. Our measurements of the microscopic single-particle dynamics show that shear thinning results from the decreased relative contribution of entropic forces and that shear thickening arises from particle clustering induced by hydrodynamic lubrication forces. This combination of techniques illustrates an approach that complements current methods for determining the microscopic origins of non-Newtonian flow behavior in complex fluids.     

Time series forecast and soil characteristics-based simple and multivariate linear models for management of Diaprepes abbreviatus root weevil in citrus

Synthesis of insect and soil variable patterns in space and time via process models would be useful for reducing the cost of field monitoring and for achieving improved integrated pest management. During 2001-2003, we monitored the Diaprepes abbreviatus (L.) root weevil population in a flatwoods citrus grove containing a sandy Alfisol and a loamy Mollisol in Hendry County, South Florida. Our objectives were to examine the multi-year correlations of the Diaprepes root weevil with soil characteristics, and to develop a time series model and soil variable-based simple and multivariate linear models for predicting weevil patterns in space and time. Adult weevils were monitored weekly using 100 Tedders traps arranged in a 30×12 m grid. Gravimetric soil water content (SWC), time-domain-reflectory (TDR) volumetric SWC, soil organic matter content (SOM), clay, sand, silt, pH, and Mehlich-I extractable P, K, Ca, Mg, Fe and Mn concentrations were determined for each trap location. Adult weevil density was 0.023±0.018 weevils m⁻² across the 3 years, and the weevil population grew exponentially (R²=0.81). Each year, the weevil was positively related to SWC, TDR, clay, SOM, and soil Mg, Ca and K concentrations (0.32<r<0.65, P<0.05). High weevil density, SWC, SOM, and P, K, Mg and Ca levels matched the Mollisol boundary. The autocorrelation ranges for the weevil and soil variables were within the soil type unit. Time series moving average forecast of Diaprepes weevil development was related to the 3-year mean weevil density monitored in the field (R²=0.88). The SWC, TDR and SOM-based simple and multivariate linear models explained 45% of the variance in the weevil patterns (P<0.001). Time series and soil characteristics-based simple and multivariate linear models suggest a variable rate and less frequent spray for future management of the weevil in citrus production systems.