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41.
Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein 总被引:29,自引:0,他引:29
W R Schafer C E Trueblood C C Yang M P Mayer S Rosenberg C D Poulter S H Kim J Rine 《Science (New York, N.Y.)》1990,249(4973):1133-1139
The post-translational processing of the yeast a-mating pheromone precursor, Ras proteins, nuclear lamins, and some subunits of trimeric G proteins requires a set of complex modifications at their carboxyl termini. This processing includes three steps: prenylation of a cysteine residue, proteolytic processing, and carboxymethylation. In the yeast Saccharomyces cerevisiae, the product of the DPR1-RAM1 gene participates in this type of processing. Through the use of an in vitro assay with peptide substrates modeled after a presumptive a-mating pheromone precursor, it was discovered that mutations in DPR1-RAM1 cause a defect in the prenylation reaction. It was further shown that DPR1-RAM1 encodes an essential and limiting component of a protein prenyltransferase. These studies also implied a fixed order of the three processing steps shared by prenylated proteins: prenylation, proteolysis, then carboxymethylation. Because the yeast protein prenyltransferase could also prenylate human H-ras p21 precursor, the human DPR1-RAM1 analogue may be a useful target for anticancer chemotherapy. 相似文献
42.
Tarduno JA Sliter WV Kroenke L Leckie M Mayer H Mahoney JJ Musgrave R Storey M Winterer EL 《Science (New York, N.Y.)》1991,254(5030):399-403
The timing of flood basalt volcanism associated with formation of the Ontong Java Plateau (OJP) is estimated from paleomagnetic and paleontologic data. Much of OJP formed rapidly in less than 3 million years during the early Aptian, at the beginning of the Cretaceous Normal Polarity Superchron. Crustal emplacement rates are inferred to have been several times those of the Deccan Traps. These estimates are consistent with an origin of the OJP by impingement at the base of the oceanic lithosphere by the head of a large mantle plume. Formation of the OJP may have led to a rise in sea level that induced global oceanic anoxia. Carbon dioxide emissions likely contributed to the mid-Cretaceous greenhouse climate but did not provoke major biologic extinctions. 相似文献
43.
Intercontinental spread of a new antibiotic resistance gene on an epidemic plasmid 总被引:10,自引:0,他引:10
T F O'Brien M P Pla K H Mayer H Kishi E Gilleece M Syvanen J D Hopkins 《Science (New York, N.Y.)》1985,230(4721):87-88
Bacteria of different genera isolated at nine medical centers in different parts of the United States and at one center in Venezuela during the first decade of gentamicin usage carried the gentamicin resistance gene 2"-aminoglycoside nucleotidyltransferase on the same transferable plasmid. Such widespread dissemination of a newly observed resistance gene on one plasmid suggests that a new resistance gene may emerge once on a single plasmid, which then carries it to other centers and other plasmids. The resistance gene might, therefore, be contained if detected early. 相似文献
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D A Carlson M S Mayer D L Silhacek J D James M Beroza B A Bierl 《Science (New York, N.Y.)》1971,174(4):76-78
A sex pheromone isolated from the cuticle and feces of the female house fly attracts the male fly; it has been identified as (Z)-9-tricosene. Chemical and biological comparisons of the natural and synthesized compounds show that they are identical. 相似文献
46.
Scanning electron microscopy and single unit recordings of male Trichoplusia ni antennae reveal at least two classes of pheromone-sensitive sensilla trichodea. The longer sensillum contains two receptor neurons each with small amounts of spontaneous activity. One neuron responds to large (10-microgram) doses of (Z)-7-dodecenyl acetate, a component of the female sex pheromone. The shorter sensillum contains two receptor neurons both with larger amounts of spontaneous activity and increased sensitivity to low (0.01-microgram) doses of pheromone. 相似文献
47.
Recent current velocity measurements across the lower continental rise of Nova Scotia show a deep equatorwardflow with speeds (maximum, 73 centimeters per second) among the highest recorded for the deep sea. Silicate measurements indicate that this flow usually consists of southern-source (Antarctic) bottom water. These measurements confirm the existence of a second and deeper western boundary flow that was earlier inferredfrom geological observations. 相似文献
48.
Radio spectral line radiation of water molecules at a wavelength of 1.35 centimeters has been measured from eight sources in the galaxy. The sources are less than I arc minute in diameter, have extremely high brightness temperatures, and show many spectral features. Some spectral features are slightly polarized and some have changed greatly in intensity in a few weeks time. 相似文献
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