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排序方式: 共有414条查询结果,搜索用时 15 毫秒
81.
Shiro Fukuta Reiko Takahashi Satoru Kuroyanagi Noriyuki Miyake Hirofumi Nagai Hirofumi Suzuki Fujio Hashizume Tomoko Tsuji Hiromi Taguchi Hideki Watanabe Koji Kageyama 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):689-701
A loop-mediated isothermal amplification (LAMP) reaction with a primer set designed from the rDNA ITS sequence of P. aphanidermatum was developed. Results of a specificity test using 57 strains of Pythium spp. indicated that the LAMP assay gave no cross reactions in other 39 Pythium species, 11 strains of Phytophthora spp. and eight other soil borne pathogens. The detection limit was 10 fg of genomic DNA, which was ten times the sensitivity of the polymerase chain reaction. The LAMP assay was applied to hydroponic solution samples from tomato fields, and the results were compared to those of the conventional plating method. LAMP was observed to be effective for the specific detection of P. aphanidermatum. Furthermore, P. aphanidermatum was detected directly in tomato roots infected with P. aphanidermatum without DNA extraction. The LAMP method established in this study is a simple, sensitive and rapid tool for the detection of P. aphanidermatum. 相似文献
82.
Takeshi Kashiwa Keigo Inami Masashi Fujinaga Hideki Ogiso Takanobu Yoshida Tohru Teraoka Tsutomu Arie 《Journal of General Plant Pathology》2013,79(6):412-421
Six4, a small protein secreted by Fusarium oxysporum f. sp. lycopersici (Fol) in tomato xylem sap during infection, triggers Fol race 1-specific resistance (I) in tomato. SIX4 is regarded as an avirulence gene. Although SIX4 is considered unique to Fol race 1, we detected this gene in the cabbage yellows fungus F. oxysporum f. sp. conglutinans (Foc) by PCR. Because the genes from Foc and SIX4 in Fol were >99 % identical at the nucleotide level, the Foc gene was designated FocSIX4. The expression of FocSIX4 was detected by RT-PCR in stems and roots of cabbage 8 days after infection with Foc. In contrast with Fol, disruption of FocSIX4 in Foc did not increase virulence to Foc-resistant cabbage cvs. Shutoku-SP and Koikaze. On the contrary, the disruptants had reduced virulence not only on Foc-resistant cultivars but also on Foc-susceptible cv. Shikidori. These results suggested that FocSIX4 is involved in virulence, but not in avirulence, in the cabbage yellows fungus. 相似文献
83.
About 20 species of viruses belonging to five genera, Benyvirus, Furovirus, Pecluvirus, Pomovirus and Bymovirus, are known to be transmitted by plasmodiophorids. These viruses have all positive-sense, single-stranded RNA genomes that consist of two to five RNA components. Three species of plasmodiophorids are recognized as vectors: Polymyxa graminis, P. betae, and Spongospora subterranea. The viruses can survive in soil within the long-lived resting spores of the vector. There are biological and genetic variations in both virus and vector species. Many of the viruses are causal agents of important diseases in major crops such as rice, wheat, barley, rye, sugar beet, potato, and groundnut. Control is dependent on the development of resistant cultivars. During the last half century, several virus diseases have rapidly spread worldwide. For six major virus diseases, we address their geographical distribution, diversity, and genetic resistance. 相似文献
84.
85.
Tsuboi M Etoh H Kato K Nakatugawa H Kato H Maejima Y Matsumoto G Mori H Hosokawa M Miyashita K Tokuda H Suzuki N Maoka T 《Journal of agricultural and food chemistry》2011,59(19):10572-10578
The in vitro reactivity of capsanthin (1) and fucoxanthin (2) with peroxynitrite was investigated, and the reaction products produced by scavenging with peroxynitrite were analyzed. (14'Z)-Nitrocapsanthin (3) and 12-nitrocapsanthin (4) were isolated from the products of the reaction of capsanthin with peroxynitrite. Similarly, (14Z)-15-nitrofucoxanthin (5), (11Z)-11-nitrofucoxanthin (6), and (14Z,9'Z)-15-nitrofucoxanthin (7) were obtained from the reaction of peroxynitrite reaction with fucoxanthin. Capsanthin and fucoxanthin inhibited the nitration of tyrosine by peroxynitrite. Furthermore, nitrocapsanthins (3 and 4) and nitrofucoxanthins (5 and 6) exhibited an inhibitory effect on Epstein-Barr virus early antigen activation in Raji cells and an antiproliferative effect on human pancreatic carcinoma. Moreover, nitrocapsanthins (3 and 4) inhibited carcinogensis of mouse skin tumors initiated by 7,12-dimethylbenz[a]anthracene (DMBN). 相似文献
86.
Yuya Kumagai Keigo Toji Satoshi Katsukura Rie Morikawa Toshiki Uji Hajime Yasui Takeshi Shimizu Hideki Kishimura 《Marine drugs》2021,19(4)
More than 7000 red algae species have been classified. Although most of them are underused, they are a protein-rich marine resource. The hydrolysates of red algal proteins are good candidates for the inhibition of the angiotensin-I-converting enzyme (ACE). The ACE is one of the key factors for cardiovascular disease, and the inhibition of ACE activity is related to the prevention of high blood pressure. To better understand the relationship between the hydrolysates of red algal proteins and the inhibition of ACE activity, we attempted to identify novel ACE inhibitory peptides from Pyropia pseudolinearis. We prepared water soluble proteins (WSP) containing phycoerythrin, phycocyanin, allophycocyanin, and ribulose 1,5-bisphosphate carboxylase/oxygenase. In vitro analysis showed that the thermolysin hydrolysate of the WSP had high ACE inhibitory activity compared to that of WSP. We then identified 42 peptides in the hydrolysate by high-performance liquid chromatography and mass spectrometry. Among 42 peptides, 23 peptides were found in chloroplast proteins. We then synthesized the uncharacterized peptides ARY, YLR, and LRM and measured the ACE inhibitory activity. LRM showed a low IC50 value (0.15 μmol) compared to ARY and YLR (1.3 and 5.8 μmol). In silico analysis revealed that the LRM sequence was conserved in cpcA from Bangiales and Florideophyceae, indicating that the novel ACE inhibitory peptide LRM was highly conserved in red algae. 相似文献
87.
Taiichiro Hattori Kaori Sonobe Hideki Araki Shinobu Inanaga Ping An Shigenori Morita 《Journal of plant nutrition》2013,36(8):1482-1495
In this study, it was verified whether silicon (Si) affected plant hydraulic resistance, which was one of the significant factors affecting water uptake. Sorghum bicolor (L.) Moench. was grown hydroponically under varying silicon levels and exposed to osmotic stresses. Under osmotic stress, reduction in growth, photosynthesis, and transpiration were alleviated as supplied silicon levels increased. These alleviative effects were ascribed to enhancement of water uptake. Although shoot/root ratio was not affected by silicon, estimated apparent hydraulic resistance was lower in silicon-supplied sorghum than silicon-deficient one under osmotic stress. Simultaneous measurement of transpiration and water uptake rates indicated that under osmotic stress silicon-deficient sorghums showed unbalanced water relation that transpiration rate exceeded water uptake rate, while they were balanced in silicon-supplied sorghums. The results indicated that silicon improved hydraulic resistance, allowing sorghum to avoid from decrease in water uptake rate that happens to silicon-deficient sorghum under water stress. 相似文献
88.
Hidehiro Inagaki Toshiyuki Imaizumi Guang-Xi Wang Tohru Tominaga Kimihiko Kato Hiroyuki Iyozumi Hideki Nukui 《Pesticide biochemistry and physiology》2009,95(3):117-120
All living organisms spontaneously generate ultraweak photon emissions, which originate from biochemical reactions in cells. Current research uses the ultraweak photon emission from organisms as a novel indicator in nondestructive analyses of an organisms living state. This study indicates that ultraweak photon emissions from Monochoria vaginalis are different between resistant biotypes (R) to sulfonylurea (SU) and susceptible biotypes (S). In SU-R biotypes, distinct increases in photon emissions were observed, but there was little increase in SU-S biotypes. In addition, photon emissions from the resistant biotypes of M. vaginalis were suppressed by treatment with P450 inhibitors. This suggests that cytochrome P450 monooxygenase, which plays a crucial role in the metabolic detoxification of SUs, could be associated with the generation of ultraweak photon emissions. Ultraweak photon emissions have a potential use in a novel diagnosis system as an indicator in a nondestructive testing of weeds resistant to SUs. 相似文献
89.
90.
ABSTRACT Two begomoviruses (Java virus-1 and Java virus-2), two satellite DNAs (DNAbeta01 and DNAbeta02), and a recombinant DNA (recDNA) were cloned from a single tomato plant from Indonesia with leaf curl symptoms, and the role of these satellite DNAs in the etiology of begomovirus disease was investigated. The genome organizations of the two viruses were similar to those of other Old World monopartite begomoviruses. Comparison of the sequences with other begomoviruses revealed that Java virus-1 was a newly described virus for which the name Tomato leaf curl Java virus (ToLCJAV) is proposed. Java virus-2 was a strain of Ageratum yellow vein virus (AYVV) (AYVV-[Java]). ToLCJAV or AYVV-[Java] alone did not induce leaf curl symptoms in tomato plants. However, in the presence of DNAbeta02, both ToLCJAV and AYVV-[Java] induced leaf curl symptoms in tomato plants. In the presence of DNAbeta01, these viruses induced mild leaf curl symptoms in tomato plants. The recDNA had a chimeric sequence, which arose from recombination among ToLCJAV, AYVV-[Java], DNAbeta01, and DNAbeta02; it was replicated only in the presence of AYVV-[Java] in tomato plants. 相似文献