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71.
72.
Effects of high dose of vitamin A on reproduction and egg quality of Japanese flounder Paralichthys olivaceus 总被引:2,自引:0,他引:2
Hirofumi Furuita Hideki Tanaka Takeshi Yamamoto Manabu Shiraishi Toshio Takeuchi 《Fisheries Science》2001,67(4):606-613
ABSTRACT: The present study was conducted to investigate the effects of dietary vitamin A on reproduction and egg quality in Japanese flounder Paralichthys olivaceus . Broodstock were fed experimental pellets containing two levels of vitamin A [11 × 103 IU/100 g (control diet; CD), 337 × 103 IU/100 g (experimental diet; ED)] for approximately 2 months before spawning and during the spawning period. Two groups of five females (average weight 1.4 kg) and 10 males (average weight 0.7 kg) were randomly allocated to two 30 m3 indoor tanks. Total egg production of the CD group was slightly higher than the ED group. Percentage of buoyant eggs and hatching rate of the ED group were significantly higher than the CD. In other egg quality parameters, such as percentage abnormal larvae and starvation tolerance of larvae, no notable difference was found between these two groups. At the end of the experiment, the skin color of broodstock in the ED group was darker than that of the CD group. Vitamin A content in eggs of the ED group was significantly higher than that of the CD group. However, the difference in vitamin A content in eggs between the ED and CD groups was much smaller than that in the liver of the females between the two groups. These results indicate that feeding broodstock a higher level of vitamin A increases the vitamin A content in eggs but does not affect egg quality in Japanese flounder because excess dietary vitamin A was stored mainly in the broodstocks' liver. 相似文献
73.
Anna Kakehashi Akihiro Hagiwara Norio Imai Min Wei Shoji Fukushima Hideki Wanibuchi 《Journal of toxicologic pathology》2015,28(1):27-32
In the present study, in continuation of our previous experiment in order to investigate the mode of action (MOA) of ethyl tertiary-butyl ether (ETBE) hepatotumorigenicity in rats, we aimed to examine alterations in cell proliferation, that are induced by short-term administration of ETBE. F344 rats were administered ETBE at doses of 0, and 1,000 mg/kg body weight twice a day by gavage for 3, 10, 17 and 28 days. It was found that the previously observed significant increase of P450 total content and hydroxyl radical levels after 7 days of ETBE administration, and 8-OHdG formation at day 14, accompanied by accumulation of CYP2B1/2B2, CYP3A1/3A2, CYP2C6, CYP2E1 and CYP1A1 and downregulation of DNA oxoguanine glycosylase 1, was preceded by induction of cell proliferation at day 3. Furthermore, we observed an increase in regenerative cell proliferation as a result of ETBE treatment at day 28, followed by induction of cell cycle arrest and apoptosis by day 14. These results indicated that short-term administration of ETBE led to a significant early increase in cell proliferation activity associated with induction of oxidative stress, and to a regenerative cell proliferation as an adaptive response, which could contribute to the hepatotumorigenicity of ETBE in rats. 相似文献
74.
Kazuhiro HARA Hideki WATABE Shinji SASAZAKI Fumio MUKAI Hideyuki MANNEN 《Animal Science Journal》2010,81(2):152-157
This study describes the development of efficient single nucleotide polymorphism (SNP) markers for individual identification and parentage tests in a Japanese Black cattle population. An amplified fragment length polymorphism method was employed to detect informative candidate markers, and yielded 44 SNP markers from 220 primer combinations. 29 unlinked SNPs were finally selected as diagnostic markers. The allelic frequencies for each marker were estimated by using PCR‐RFLP in the Japanese Black population. Based on the frequency data, the estimated identity power of these markers was 2.73 × 10?12. Parentage exclusion probabilities, when both suspected parents' genotypes were known and when only one suspected parent was genotyped, were estimated as 0.96929 and 0.99693, respectively. This panel of SNP markers is theoretically sufficient for individual identification, and would also be a powerful tool for a parentage test in Japanese Black cattle. The markers could contribute to the management of the beef industry in Japan. 相似文献
75.
Hideki Watanabe Koji Kageyama Yoshihiro Taguchi Hayato Horinouchi Mitsuro Hyakumachi 《Journal of General Plant Pathology》2008,74(6):417-424
Pythium helicoides, P. aphanidermatum and P. myriotylum are important pathogens that cause root rot of several crops in hydroponic culture and in ebb-and-flow irrigation systems.
These species belong to a group of Pythium species that can grow at temperatures higher than 40°C. We developed a method for baiting these high-temperature Pythium species and evaluated its practicality to monitor their presence in nutrient solutions. Seeds of cucumber, tomato, radish,
hemp, perilla and millet and leaves of bent grass and rose were tested as baits in hydroponic systems. Hemp, perilla and radish
seeds and bent grass and rose leaves were more effective than the other baits for Pythium zoospores, and bent grass leaves were the most effective. In a sensitivity test, bent grass leaf traps (BLTs) detected three
Pythium species after only a 1 day exposure to suspensions of 40 zoospores per liter of water, and the frequency of detection increased
with zoospore density and with baiting period. A temperature of 38°C was optimum for the selective reisolation of the high-temperature
Pythium species from the BLTs. The BLT was also tested with inoculated and noninoculated miniature roses that shared a recirculating
nutrient solution. The pathogen was detected in the nutrient solution 23 days before the disease spread to the noninoculated
roses. In addition, P. helicoides was detected 30 days before the disease was evident in a commercial greenhouse. The baiting method described here will be
useful for monitoring high-temperature Pythium species in recirculating hydroponic culture systems. 相似文献
76.
Ogasawara H Ohwada S Nagai Y Taketa Y Matsuzaki M Tanaka S Watanabe K Aso H Yamaguchi T 《Domestic animal endocrinology》2008,35(1):8-15
The present study was carried out to detail the cellular localization of leptin (Lep) and the leptin receptor (LepR) in the bovine adenohypophysis. Lep immunoreactivity (Lep-ir) was found in about 30% of adenohypophysial cells in the gland. Immunochemistry of Lep and specific hormones using serial sections revealed that Lep-ir was present in 60.4% of somatotrophs, 15.9% of gonadotrophs, 6.5% of mammotrophs, 6.5% of thyrotrophs and 2.4% of corticotrophs. Both the common short isoform (OBRa) and the long isoform (OBRb) of LepR mRNA were expressed in the bovine adenohypophysis. LepR immunoreactivity (LepR-ir) was found in only 2.8% of the adenohypophysial cells and over 50% of LepR-ir cells were gonadotrophs, in which most of the cells were distributed in the zona tuberalis. The findings on Lep and LepR in the adenohypophysial cells indicate that Lep may regulate gonadotroph function through autocrine/paracrine pathway in the bovine adenohypophysis. 相似文献
77.
Yasushi Ishiguro Kayoko Otsubo Hideki Watanabe Mikihiko Suzuki Kiichi Nakayama Takashi Fukuda Masashi Fujinaga Haruhisa Suga Koji Kageyama 《Journal of General Plant Pathology》2014,80(5):423-429
Pythium species were isolated from seedlings of strawberry with root and crown rot. The isolates were identified as P. helicoides on the basis of morphological characteristics and sequences of the ribosomal DNA internal transcribed spacer regions. In pathogenicity tests, the isolates caused root and crown rot similar to the original disease symptoms. Multiplex PCR was used to survey pathogen occurrence in strawberry production areas of Japan. Pythium helicoides was detected in 11 of 82 fields. The pathogen is distributed over six prefectures. 相似文献
78.
Masuda T Odaka Y Ogawa N Nakamoto K Kuninaga H 《Journal of agricultural and food chemistry》2008,56(2):597-601
Lemongrass is a popular Asian herb having a lemon-like flavor. Very recently, potent tyrosinase inhibitory activity has been found in lemongrass in addition to various biological activities reported in the literature. The aim of the present study is to identify the active compounds in the lemongrass. An assay-guided purification revealed that one of the active substances was geranic acid. Geranic acid has two stereoisomers, which are responsible for the trans and cis geometry on the conjugated double bond. Both isomers are present in the active ethyl acetate-soluble extract of the lemongrass, and their IC50 values were calculated to be 0.14 and 2.3 mM, respectively. The structure requirement of geranic acid for the potent tyrosinase inhibitory activity was investigated using geranic acid-related compounds. 相似文献
79.
Effects of a high milk intake during the pre‐weaning period on nutrient metabolism and growth rate in Japanese Black cattle 下载免费PDF全文
Atsuko Matsubara Hideyuki Takahashi Akira Saito Aoi Nomura Khounsaknalath Sithyphone Christopher D. Mcmahon Ryoichi Fujino Yuji Shiotsuka Tetsuji Etoh Mitsuhiro Furuse Takafumi Gotoh 《Animal Science Journal》2016,87(9):1130-1136
This study aimed to determine the effects of feeding an increased volume of high‐fat milk during the early post‐natal life on metabolite concentrations in the blood, the expression of key genes regulating intermediary metabolism in the skeletal muscles, and the rate of growth of Japanese Black cattle. All calves were fed a high‐fat milk replacer (crude protein, 26%; crude fat, 25.5%; total dissolved nitrogen, 116%). Control calves (n = 4) were nursed with 500 g milk replacer until 3 months of age, whereas calves in the experimental group (n = 4) were nursed with 1800 g milk replacer until 3 months, and then the volume was gradually reduced until 5 months. Body weight was significantly higher in the experimental group than in the control group at 7 months. Plasma glucose concentrations were significantly lower in the experimental group. Expression of glucose‐transporter‐4 messenger RNA (mRNA) was lower, whereas that of glucose transporter 1, cluster of differentiation 36, and carnitine palmitoyltransferase‐1b mRNA was significantly higher in the Longissimus thoracis of the experimental group. Nutritional status during early post‐natal life appears to strongly influence the growth rate and glucose and lipid metabolism in Japanese Black cattle. 相似文献
80.
Ayano OMURA Wataru ANZAI Daisuke KOYABU Hideki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1043-1048
Clarification of the trunk structure in Urodela is important in understanding the
locomotive evolution of basal tetrapods. The components of the muscular trunk wall among
Urodela using different modes of locomotion were compared. Since the whole trunk may be
used for swimming and the effect of limbs may be small in the more aquatic species, they
showed smaller differences in the trunk muscles among anterior, middle and posterior
sections of the trunk. By contrast, in the more terrestrial species, the dorsal and
abdominal muscles are larger in the middle section than those in the anterior and
posterior sections. High compressive stresses occur in the supporting limbs and their
insertion at the trunk on the ventral side, and spread from the forelimbs along the back
to the supporting hindlimbs on the dorsal side. Tensile stresses occur in the middle
ventral part. The components of the trunk muscles among the three sections may reflect
differences in stresses occurring in the trunk of the more terrestrial species. The
findings also suggest that in the middle section, larger dorsal muscles for stiffening the
back to maintain posture and larger abdominal muscles are responsible for balancing the
body weight while it is supported by the limbs in the more terrestrial species. 相似文献