Occurrence of <Emphasis Type="Italic">Bursaphelenchus mucronatus</Emphasis> (Nematoda; Aphelenchoididae) in France and association with <Emphasis Type="Italic">Monochamus galloprovincialis</Emphasis> (Coleoptera: Cerambycidae)

As a consequence of the recent introduction of the pine wood nematode Bursaphelenchus xylophilus in Portugal, nematodes of the genus Bursaphelenchus were looked for in various French pine forests, in trees attacked by Monochamus galloprovincialis, the vector insect of B. xylophilus, and in the insects themselves. Trap trees were felled in 12 localities distributed all over the country. Nematodes were extracted from transversal stem discs; insects emerging from the trap trees were studied. B. hellenicus, B. leoni, B. mucronatus and B. sexdentati were isolated, but not B. xylophilus. The presence of B. mucronatus and the absence of B. xylophilus were confirmed by molecular markers. B. mucronatus was isolated from several regions with an average prevalence of 19%. The infestation of M. galloprovincialis by B. mucronatus reached 26.7%. The wide distribution of B. mucronatus in France could have an effect on the extension of B. xylophilus in a case of an introduction.     

Influence of Puroindolines A and B Individually and in Combination on Wheat Milling and Bread Traits

Endosperm texture in wheat (Triticum aestivum L.) is determined by the Pina and Pinb genes located within the Hardness (Ha) locus on chromosome 5D. We have previously shown that Pina and Pinb can act alone to produce intermediate-textured grain or act together to produce soft grain. The objective here was to isolate the role of PINA and PINB individually and in combination on milling and bread traits by analyzing F₃ recombinant lines created by crosses between PINA and PINB null cultivars with Pina-D1a and Pinb-D1a overexpressing transgenic lines. Homozygous lines that contained either the Pina-D1b/Pinb-D1a (Pina null) or Pina-D1a/Pinb-D1e (Pinb null) Ha locus with or lacking transgenically added Pina or Pinb were analyzed for milling and bread traits. Addition of Pina-D1a to Pina-D1b/Pinb-D1a and addition of Pinb-D1a to Pina-D1a/Pinb-D1e Ha locus genotypes gave soft grain with lower flour yield, flour ash, and a higher proportion of small flour particles. Addition of Pinb-D1a produced greater negative effects on loaf volume than addition of Pina-D1a. Grain hardness, flour protein, flour ash, and mixograph water absorption were positively correlated, which is indicative of the complex phenotype conditioned by PINs. The results demonstrate that PIN overexpression leads to a reduction in grain hardness and reduced flour yield, flour ash, and flour particle size. PIN expression also results in reduced loaf volume and flour water absorption.     

Synthesis and fungicidal activity of N-2-(3-methoxy-4-propargyloxy)phenethyl amides. Part II: anti-oomycetic mandelamides

Novel types of anti-oomycetic compounds have been designed and prepared. The synthetic approach to these mandelamides is outlined. Biological data demonstrate their high efficacy against important plant diseases such as tomato and potato late blight (Phytophthora infestans De Bary) and grape downy mildew (Plasmopara viticola Berliner & de Toni). Structure-activity relationship studies are discussed. The new development product mandipropamid is presented.     

Shoot biomass growth is related to the vertical leaf nitrogen gradient in Salix canopies

Plant canopy optimization models predict that leaf nitrogen (N) distribution in the canopy will parallel the vertical light gradient, and numerous studies with many species have confirmed this prediction. Further, it is predicted that for a given canopy leaf area, a low vertical light extinction coefficient will promote rapid growth. Therefore, the ideal canopy of fast-growing plants should combine high leaf area index with a low light extinction coefficient; the latter being reflected in a flat vertical leaf N gradient throughout the canopy. Based on data from an experimental Salix stand (six varieties) grown on agricultural land in central Sweden, we tested the hypothesis that shoot growth is correlated with vertical leaf N gradient in canopies of hybrid willows bred for biomass production, which could have implications for Salix breeding. Tree improvement research requires screening of growth-related traits in large numbers of plants, but assessment of canopy leaf N gradients by chemical analysis is expensive, time-consuming and destructive. An alternative to analytical methods is to estimate leaf N gradients nondestructively with an optical chlorophyll meter (SPAD method). Here we provide a specific calibration for interpreting SPAD data measured in hybrid willows grown in biomass plantations on fertile agricultural land. Based on SPAD measurements, a significant and inverse relationship (r(2) = 0.88) was found between shoot biomass growth and vertical leaf N gradient across canopies of six Salix varieties.     

Initial Experience with Norepinephrine Infusion in Hypotensive Critically III Foals

Seven critically ill foals that continued to be hypotensive despite fluid resuscitation and the infusion of dobutamine and/or dopamine were treated with an infusion of norepinephrine (noradrenaline). The norepinephrine was administered concurrently with dobutamine, and the combination therapy was titrated by use of indirect mean arterial pressure measurements. The highest dose of norepinephrine used was 1.5 mcg/kg/min. In six foals the administration of norepinephrine was associated with an increase in blood pressure. In one foal the mean arterial pressure did not increase in response to the doses of norepinephrine administered. All of the foals experienced an increase in urine output coincident with the start of the norepinephrine infusion. Three of the foal survived to hospital discharge.     

DUPLEX-ULTRASONOGRAPHIC EVALUATION OF THE COMMON CAROTID ARTERY IN THE RESTING, SEDATED AND ANESTHETIZED HORSE

The aim of this study was to determine the blood flow in the carotid artery during halothane anaesthesia by means of duplex-sonography. Seventeen warm blood horses were evaluated in dorsal and lateral recumbency and the results were compared with the values of the same horses resting and under sedation. The cross sectional area of the vessels, the time-averaged maximal blood velocity, time-averaged maximal blood flow and the resistance index were determined and the flow profile was evaluated. During halothane anesthesia the total blood flow shows a significant increase which is not dependent on the positioning of the horse. Mean blood velocity is decreased by sedation and significantly increased during anesthesia. Reduced peripheral resistance is expressed by a decline of the resistance index. After sedation and during recumbency there is an increase in diameter of the carotid artery.     

Two-Year Weed Seedling Population Responses to a Post-Emergent Method of Site-Specific Weed Management

Field experiments were conducted to determine how a site-specific weed management practice in Zea mays L. influenced the numerical and spatial distribution of a naturally occurring weed infestation in Z. mays and the succeeding Beta vulgaris L. crop. Compared to conventional broadcast herbicide applications, site-specific herbicide applications reduced herbicide load by 11.5 and 98.0% in two separate Z. mays fields. The broad range in outcomes was attributed to the spatial aggregation and density of target weed populations. While herbicide use was successfully reduced at field locations with low weed density, most survivors of multiple control tactics were in locations with the highest initial density. A greater understanding of interactions between weed management and weed density would increase the likelihood that site-specific weed management offers long-term improvements over conventional approaches.     

Effects of pre-treatment, renovation procedure and cultivar on the growth of white clover sown into a permanent pasture under both grazing and mowing regimes

White clover ( Trifolium repens ) is a valuable pasture component that is frequently present in insufficient quantity for optimal animal nutrition. Several methods of reintroducing white clover into a permanent pasture without conventional tillage were investigated. Three seeders (Hunter, Vredo and a conventional seed drill), two white clover cultivars (Sacramento, and Sonja), two pasture pre-treatments (a hard spring grazing or grazing plus light harrowing) and two defoliation regimes (grazing or mowing) were used to determine optimal seedling establishment conditions. Defoliation treatments were used as a method of investigating pasture improvement experiments. Measurements were taken to determine proportion of white clover present and total herbage mass.
Plots renovated using a Hunter drill had the highest white clover content in the months immediately after renovation. Subsequently pre-treatment method appeared to have no significant effect on herbage mass or species composition. The proportion of white clover in plots sown with the cultivar Sacramento was frequently higher than that in plots sown with the cultivar Sonja, but, overall, herbage production of cultivars was not different.
Mowed plots had higher herbage production and tended towards a greater white clover content than grazed plots. Compaction of the surface to a depth of 10 cm in the grazed plots may have been a factor in the observed difference in herbage production. Regardless of management, within two years white clover content was similar among all treatments, including controls.     

Responses of the parthenogenetic isopod, Trichoniscus pusillus (Isopoda: Oniscidea), to changes in food quality

Long-term observations of Trichoniscus pusillus revealed that chemical composition and microbial activity of the leaf litter serving as food influenced the reproductive success of females and the mortality rates of juveniles as well. Of the food sources investigated, only alder litter, exhibiting high microbial activity, allowed the maintenance of a stable population with frequently reproducing females. This study concludes that the food type is a significant factor for population parameters of this parthenogenetic isopod. Our results on T. pusillus, belonging to the taxon Synocheta, are compared with data on terrestrial isopod species of the taxon Crinocheta.