Failure of procaine penicillin and zinc bacitracin to modify the response of the fowl to stressors.

1. The effect of either procaine penicillin or zinc bacitracin (25 mg active base/kg food) on the response of young birds to three different stressors - withdrawal of food for 18 h, exposure to cold for 24 h or treatment with ACTH- has been examined. 2. Neither antibiotic had any consistent effect on the stress responses. 3. It is concluded that neither penicillin nor bacitracin has any stress-ameliorating activity at least under the conditions of the test.     

Characterization of the sorption of an anthranilate fungicide in soil using thermal analytical and mineralogical techniques

The sorption of pesticides to soil particles has implications for their distribution and fate in the soil environment. A batch equilibrium technique was used to investigate sorption of the fungicide AEC623892 to intact and hydrogen-peroxide-treated whole soils and several particle-size fractions isolated from them. Sorption of AEC623892 to the soil as a whole was low. K(f oc) values measured in the whole soils were 169.2 and 41.9 ml g(-1) for Soil A and Soil B respectively. The highest values of K(f oc) were measured in soil particle-size fractions <53 microm (266.5 ml g(-1) in the 2-20 microm fraction of Soil A; 471.9 ml g(-1) in the 20-53 microm fraction of Soil B). Sorption was most irreversible in the 2-20 microm fractions. Overall, treatment of soil particle-size fractions with hydrogen peroxide resulted in lower values of K(f oc) (112.3 ml g(-1) in Soil A whole soil and 30.9 ml g(-1) in Soil B whole soil). In both soils, the maximum sorption among hydrogen-peroxide-treated samples was observed in the <2 microm fraction (166.6 and 311.0 ml g(-1) for Soil A and Soil B, respectively). Investigation of the mineralogical composition of the soils suggested that the clay mineralogy (dominated by kaolinite and illite) is less likely to account for the differences in sorption observed than differences in the characteristics of the soil organic matter. Thermal analysis of the different soil fractions indicated that hydrogen peroxide treatment preferentially removed aliphatic fractions of organic matter, but had less effect on lignin-like, aromatic fractions.     

Performance of a Johne's disease enzyme-linked immunosorbent assay adapted for milk samples from goats.

Antibody detection-based tests for paratuberculosis offer speed and economy, 2 diagnostic test attributes important to animal industries with narrow profit margins. Application of such tests to individual milk samples instead of serum samples can further improve testing efficiency and decrease testing cost. Accuracy of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay (ELISA) adapted for use on goat serum and milk samples was determined. Fecal, blood, and milk samples were collected from 159 goats belonging to 2 Wisconsin goat herds with a prior history of paratuberculosis and 1 herd of 50 goats from a paratuberculosis-free Wisconsin herd. Fecal samples were cultured using the modified BACTEC 12B media. Sera were tested according to the manufacturer's instructions for bovine samples. Milk samples were centrifuged and mixed with the ELISA kit's Mycobacterium phlei-containing diluent at a ratio of 1:2. Using fecal culture as the "gold standard," the sensitivity of the ELISA on goat serum was 64% and the sensitivity of the ELISA on goat milk was 48%. The milk ELISA had higher agreement with fecal culture results (kappa = 0.525) than the serum ELISA (kappa = 0.425). ELISA specificity was 100% on both serum and milk. Regression analysis also showed good correlation between serum and milk S/P values (r2 = 0.67). Although less sensitive, the ELISA on goat milk samples appears to offer a useful, low-cost alternative for detection of goats with paratuberculosis that have progressed to the stage of shedding M. paratuberculosis in their feces.     

Dietary aureomycin and the response of the fowl to stressors.

1. Conventional or gnotobiotic chicks, when injected from 1 d to 3 weeks of age with adrenocorticotrophic hormone (120IU/kg, three times weekly), showed a depressed growth rate, adrenal hypertrophy and depletion of cholesterol form the adrenal glands. 2. Feeding a diet supplemented with aureomycin (10 mg/kg) did not have any consistent ameliorating effect on the response of the stressed bird as judged by the above parameters. 3. It was found that treating germfree chicks with five daily injections of sterile milk on days 3 to 7 did not depress growth rate at any time, nor could differences in adrenal size or cholesterol stores be detected at the end of the 21-d experimental period. The responses were not modified by feeding an aureomycin-supplemented diet.     

Anaerobic metabolism of DDT analogs by pigeon liver preparations

The amounts of p,p′-DDT and of five other trichloroethane derivatives decreased upon incubation under anaerobic conditions with 12,000g × 30 min pigeon liver supernatant fraction. The addition of an exogenous NADPH-generating system sometimes, but not invariably, increased the rate of metabolism. Only one hexane-soluble metabolite was detected in the postincubation reaction medium of each of the six trichloroethane derivatives. After isolation by tlc the six metabolites were shown by mass spectrometry to have molecular weights 34 units less than their parent compounds. Comparison of the isotope patterns in the spectra of each substrate and its metabolite reveals that in each case the metabolism involves the loss of a chlorine atom. From these data it is concluded that several substituted trichloroethanes undergo reductive dechlorination when they are incubated with liver preparations in an atomosphere of nitrogen. Two dichloroethane derivatives, tested in a similar manner, were unchanged and were recovered quantitatively. Mass spectrometric and chromatographic data of reactants and products are recorded.     

The response of the immature fowl to multiple injections of adrenocorticotrophic hormone.

1. Treating chicks, from 1 d or 5 weeks of age with ACTH three times weekly for 3 weeks depresses growth and causes adrenal hypertrophy at dose rates of 30 IU/kg or more, and depletion of adrenal cholesterol (greater than 10 IU/kg). 2. Treating chicks five times weekly at a dose rate of 30 IU/kg was as effective as a dose of 120 IU/kg three times weekly. 3. Plasma glucose and FFA concentrations of chicks treated thrice weekly with 120 IU ACTH/kg for 3 weeks were within the normal range. 4. Rhode Island Reds were more sensitive to ACTH than Light Sussex. 5. Adrenal cholesterol stores in normal chicks show significant variations with season.     

Parotid duct transposition in dogs: a retrospective review of 92 eyes from 1999 to 2009

Objective The aim of this study was to perform a retrospective review of parotid duct transposition (PDT) in the dog to determine the rate and nature of complications, the success and failure rates and to evaluate owner satisfaction. Methods Medical records of 56 dogs (92 eyes) that underwent PDT and subsequent follow‐up by a veterinary ophthalmologist were reviewed. Forty owners (40 dogs/66 eyes) were contacted by telephone and 37 owners (37 dogs/60 eyes) also completed a visual analog scale questionnaire. Statistical evaluation included Wilcoxon Signed Rank tests, one‐way analysis of variance and Kaplan–Meier survival analysis with Wilcoxon and Log‐rank tests. Results The mean follow‐up was 38.7 months (range 1–109 months). The surgical success rate was 92% (85/92 eyes). Total failures (8%, 7/92 eyes) were because of severe saliva intolerance (n = 5 eyes) and PDT failure (n = 2 eyes). The complication rate was 50% (46/92 eyes) of which 61% (28/46 eyes) were managed medically and 39% (18/46 eyes) required further surgery. Ninety percent (36/40) of owners indicated that they would proceed with surgery again. Statistically significant improvements in owner perception of ocular comfort, number of daily topical treatments, ocular wetness, and postoperative vision were identified. Conclusions This study shows that PDT is a successful procedure based on clinical findings and in terms of owner perception. It has also demonstrated that PDT improves ocular comfort and vision in medically refractive cases of keratoconjunctivitis sicca, and that a low level of on‐going management is required in 33% of cases.     

Use of pyrolysis/GC–MS combined with thermal analysis to monitor C and N changes in soil organic matter from a Mediterranean fire affected forest

In Mediterranean ecosystems, forest fires are a common phenomenon. They involve the transformation of vegetation and litter, leaving charred residues and so influencing the carbon cycle by changing (a) the amounts of soil organic matter and (b) the proportions within it of pools with differing stability. In addition to affecting C cycles, fires also affect the amounts of N within soil organic matter, and its availability.