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41.
    
Imiquimod is an immune response modifier with potent antiviral and antitumour activity. The objective of this pilot study was to evaluate the efficacy of an imiquimod 5% cream (Aldaratrade mark: 3M, Saint Paul, MN, USA) as a topical treatment for equine sarcoids. Fifteen horses with a total of 19 tumours were enrolled, including mixed (7), fibroblastic (5), flat (3), verrucous (2), and nodular (2) types. Baseline data included history, physical examination, tumour location, measurement and digital photography. Imiquimod was applied by the owners three times a week until complete resolution of the tumour or 32 weeks, whichever occurred first. Tumours were measured and photographed every 4 weeks. Treatment efficacy was defined as 75% or greater reduction of tumour size by the end of the trial. Four sarcoids were withdrawn from the study. Twelve of the remaining 15 tumours (80%) showed more than 75% reduction in size and nine (60%) totally resolved between 8 and 32 weeks. The most common adverse effects of exudation, erythema, erosions, depigmentation and alopecia were limited to the tumour and adjacent areas. The results suggest that topical imiquimod is a therapeutic option for the treatment of equine sarcoids, although more detailed studies are required to corroborate these initial findings.  相似文献   
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Water is the most important limiting factor of wheat (Triticum aestivum L.) and maize (Zea mays L.) double cropping systems in the North China Plain (NCP). A two-year experiment with four irrigation levels based on crop growth stages was used to calibrate and validate RZWQM2, a hybrid model that combines the Root Zone Water Quality Model (RZWQM) and DSSAT4.0. The calibrated model was then used to investigate various irrigation strategies for high yield and water use efficiency (WUE) using weather data from 1961 to 1999. The model simulated soil moisture, crop yield, above-ground biomass and WUE in responses to irrigation schedules well, with root mean square errors (RMSEs) of 0.029 cm3 cm−3, 0.59 Mg ha−1, 2.05 Mg ha−1, and 0.19 kg m−3, respectively, for wheat; and 0.027 cm3 cm−3, 0.71 Mg ha−1, 1.51 Mg ha−1 and 0.35 kg m−3, respectively, for maize. WUE increased with the amount of irrigation applied during the dry growing season of 2001-2002, but was less sensitive to irrigation during the wet season of 2002-2003. Long-term simulation using weather data from 1961 to 1999 showed that initial soil water at planting was adequate (at 82% of crop available water) for wheat establishment due to the high rainfall during the previous maize season. Preseason irrigation for wheat commonly practiced by local farmers should be postponed to the most sensitive growth stage (stem extension) for higher yield and WUE in the area. Preseason irrigation for maize is needed in 40% of the years. With limited irrigation available (100, 150, 200, or 250 mm per year), 80% of the water allocated to the critical wheat growth stages and 20% applied at maize planting achieved the highest WUE and the least water drainage overall for the two crops.  相似文献   
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  总被引:1,自引:0,他引:1  
A system correlating climate with the annual risk of fascioliasis in cattle in central Louisiana was developed, using the Thornthwaite water budget and a 6-year data base that included records on herd prevalence rates, transmission to fluke-free sentinel calves, and snail population dynamics. The system developed was compared with modifications of the wet-day and Mt forecasting systems previously developed for use in the oceanic climate zone of western Europe. The wet-day system correlated poorly with transmission data, whereas the Mt and Thornthwaite water budget-derived systems correctly ranked 5 of the 6 years in terms of annual numbers of flukes transmitted to sentinel calves. Compared with the Mt system, the water budget-based system more closely correlated with transmission data, on the basis of statistical regression analysis. The water budget system was calculated by use of a microcomputer software program, and provided a means of measuring the effect of moisture stress and flooding in snail habitats that serve as foci of transmission of Fasciola hepatica to cattle on pastures.  相似文献   
45.
Classical metapopulation theory (CMT) has proven an attractive paradigm for ecologists concerned with the conservation of aquatic-breeding amphibians, given its apparent fit with the population dynamics of these animals, and the opportunities the concept provides to assess alternate management options. Nevertheless, several authors have cautioned against uncritical application of this paradigm. We assessed the application of CMT to the conservation of the endangered growling grass frog (Litoria raniformis) in the urbanising landscapes of Melbourne, Victoria, Australia. Support for five predictions developed from the basic tenets of CMT was assessed using a multi-year occupancy and mark–recapture data-set. There was congruence between all five predictions and data. Wetland occupancy was strongly influenced by the proximity of neighbouring populations (‘connectivity’), but the estimated rate of dispersal between wetlands was low. Wetland occupancy was also temporally dynamic, with only a weak effect of connectivity on the probability of extinction, but a strong positive influence of connectivity on the probability of colonisation. Our work confirms that CMT provides a useful model of the dynamics of L. raniformis in urbanising landscapes, and justifies the application of the paradigm to conservation planning for this species. We argue that CMT may prove relevant to numerous aquatic-breeding amphibians, and encourage assessment of the application of CMT to the conservation of these animals.  相似文献   
46.
    
Within-event variability in rainfall intensity may affect pesticide leaching rates in soil, but most laboratory studies of pesticide leaching use a rainfall simulator operating at constant rainfall intensity, or cover the soil with ponded water. This is especially true in experiments where macropores are present--macroporous soils present experimental complexities enough without the added complexity of variable rainfall intensity. One way to get around this difficulty is to use a suitable pesticide transport model, calibrate it to describe accurately a fixed-intensity experiment, and then explore the affects of within-event rainfall intensity variation on pesticide leaching through macropores. We used the Root Zone Water Quality Model (RZWQM) to investigate the effect of variable rainfall intensity on alachlor and atrazine transport through macropores. Data were used from an experiment in which atrazine and alachlor were surface-applied to 30 x 30 x 30 cm undisturbed blocks of two macroporous silt loam soils from glacial till regions. One hour later the blocks were subjected to 30-mm simulated rain with constant intensity for 0.5 h. Percolate was collected and analyzed from 64 square cells at the base of the blocks. RZWQM was calibrated to describe accurately the atrazine and alachlor leaching data, and then a median Mid-west variable-intensity storm, in which the initial intensity was high, was simulated. The variable-intensity storm more than quadrupled alachlor losses and almost doubled atrazine losses in one soil over the constant-intensity storm of the same total depth. Also rainfall intensity may affect percolate-producing macroporosity and consequently pesticide transport through macropores. For example, under variable rainfall intensity RZWQM predicted the alachlor concentration to be 2.7 microg ml(-1) with an effective macroporosity of 2.2 E(-4) cm(3) cm(-3) and 1.4 microg ml(-1) with an effective macroporosity of 4.6 E(-4) cm(3) cm(-3). Percolate-producing macroporosity and herbicide leaching under different rainfall intensity patterns, however, are not well understood. Clearly, further investigation of rainfall intensity variation on pesticide leaching through macropores is needed.  相似文献   
47.
    
The Root Zone Water Quality Model (RZWQM) is a comprehensive, integrated physical, biological and chemical process model that simulates plant growth and movement of water, nutrients and pesticides in a representative area of an agricultural system. We tested the ability of RZWQM to predict surface runoff losses of atrazine, alachlor, fenamiphos and two fenamiphos oxidative degradates against results from a 2-year mesoplot rainfall simulation experiment. Model inputs included site-specific soil properties and weather, but default values were used for most other parameters, including pesticide properties. No attempts were made to calibrate the model except for soil crust/seal hydraulic conductivity and an adjustment of pesticide persistence in near-surface soil. Approximately 2.5 (+/- 0.9), 3.0 (+/- 0.8) and 0.3 (+/- 0.2)% of the applied alachlor, atrazine and fenamiphos were lost in surface water runoff, respectively. Runoff losses in the 'critical' events--those occurring 24 h after pesticide application--were respectively 91 (+/- 5), 86 (+/- 6) and 96 (+/- 3)% of total runoff losses for these pesticides. RZWQM adequately predicted runoff water volumes, giving a predicted/observed ratio of 1.2 (+/- 0.5) for all events. Predicted pesticide concentrations and loads from the 'critical' events were generally within a factor of 2, but atrazine losses from these events were underestimated, which was probably a formulation effect, and fenamiphos losses were overestimated due to rapid oxidation. The ratios of predicted to measured pesticide concentrations in all runoff events varied between 0.2 and 147, with an average of 7. Large over-predictions of pesticide runoff occurred in runoff events later in the season when both loads and concentrations were small. The normalized root mean square error for pesticide runoff concentration predictions varied between 42 and 122%, with an average of 84%. Pesticide runoff loads were predicted with a similar accuracy. These results indicate that the soil-water mixing model used in RZWQM is a robust predictor of pesticide entrainment and runoff.  相似文献   
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In southern Australia, oriental mustard (Sisymbrium orientale) has been controlled successfully by triazine herbicides for several decades. The screening of 40 populations that were collected from the southern grain belt of Australia during 2010 and 2013 for resistance to six different herbicides (glyphosate, diflufenican, imazamox, chlorsulfuron, atrazine and 2,4‐dichlorophenoxyacetic acid) identified two oriental mustard populations as highly resistant to atrazine. Compared to the known oriental mustard‐susceptible populations (S1 and S2), these two resistant populations (P17 and P18) from near Horsham, Victoria, Australia, were 311‐ and 315‐fold resistant to atrazine, as determined by a comparison of the LD50 values. However, there was no resistance to diuron detected in these populations. Sequencing of the chloroplast psbA gene identified a missense mutation of serine 264 to glycine in both herbicide‐resistant oriental mustard populations, which is known to confer high‐level atrazine resistance in other species.  相似文献   
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