Use of remote sensing and geographical information systems to identify environmental features that influence the distribution of paramphistomosis in sheep from the southern Italian Apennines

A geographic information system (GIS) was constructed using remote sensing (RS) and landscape feature data together with Calicophoron daubneyi positive survey records from 197 georeferenced ovine farms with animals pasturing in a 3971 km(2) area of the southern Italian Apennines. The objective was to study the spatial distribution of this rumen fluke, identify environmental features that influence its distribution, and develop a preliminary risk assessment model. The GIS for the study area was constructed utilizing the following environmental variables: normalized difference vegetation index (NDVI), land cover, elevation, slope, aspect, and total length of rivers. These variables were then calculated for "buffer zones" consisting of the areas included in a circle of 3 km diameter centered on 197 farms. The environmental data obtained from GIS and RS and from data taken by the veterinarians on the field (stocking rate and presence of streams, springs and brooks on pasture) were analyzed by univariate (Spearman and ANOVA) and multivariate (discriminant) statistical analyses using the farm coprological status (positive/negative) as the dependent variable. Sheep on 32 of the 197 (16.2%) farms, were positive for C. daubneyi, with an average intensity of 52 epg. A multivariate stepwise discriminant analysis model was developed that included moors and heathland, sclerophyllous and coniferous forest vegetation, autumn-winter NDVI and presence of streams, springs and brooks on pasture. The variables entered in the model were also correlated with C. daubneyi positive farms in the univariate tests and are consistent with the environmental requirements of C. daubneyi and its snail intermediate host.     

Effect of refined soy oil or whole soybeans on intake, methane output, and performance of young bulls

An experiment was conducted to establish the effects of feeding refined soy oil (RSO) or whole soybeans (WSB) containing soy oil on DMI, animal performance, and enteric methane (CH4) emissions in young bulls. Thirty-six Charolais and Limousin cross-bred, young beef bulls (338 +/- 27 kg of BW, 218 +/- 17 d of age at the beginning of the experiment) were blocked by BW, age, and breed before being assigned in a randomized complete block design to 1 of 3 experimental treatments (n = 12). The experimental period lasted for 103 d, with enteric CH4 output recorded for 2 periods of 5 consecutive days on d 37 to 41 and d 79 to 83. The 3 dietary treatments consisted of a barley/soybean meal-based concentrate with 0 g/d of RSO; oil from WSB as 6% of DMI (WSB treatment); and oil from RSO as 6% of DMI (RSO treatment). Each diet had a 10:90 forage:concentrate ratio, using barley straw as the forage source. Diet affected DMI (P < or = 0.001) and GE intake (P < 0.05 during the CH4 measurement periods), with the WSB treatment producing the lowest values. The addition of WSB decreased ADG (P < 0.05) compared with the RSO treatment. The WSB treatment also decreased (P < 0.05) average daily carcass gain (ADCG). Both the RSO and WSB concentrates decreased (P < 0.05 to P < 0.001) daily enteric CH4 output when expressed in terms of liters per day, liters per kilogram of DMI, percentage of GE intake, liters per kilogram of ADG, and liters per kilogram of ADCG. Diet had no effect (P = 0.557) on ruminal protozoal numbers. The reductions in enteric CH4 were achieved at relatively high oil inclusion levels. Such oil levels have previously been reported to decrease DMI of high-forage diets, although no effect on DMI was noted with the low-forage diets fed in this experiment. This impact on DMI of high-forage diets may limit the range of diets for which this CH4 reduction strategy may be applicable. The inclusion level of WSB in the current experiment (27%) was beyond the palatability threshold of the bulls used and resulted in a marked decline in intake and performance. Therefore, WSB may have a role to play in ruminant diets, but only at a reduced inclusion rate.     

Managing chronic arthritis.

Many compounds are being investigated for the control of symptoms of osteoarthritis in people and animals. Ideally, treatment should include analgesia, inflammation control, and chondroprotection. With further progress in this area, combination therapies tailored to the needs of the individual animal should enable us to maximize efficacy and minimize side effects. Only a few of the newer therapies and pharmaceutic agents have been investigated in the horse, however. With more rigorous investigation, they may be determined to be ineffective or unsafe. Meanwhile, as much information should be gathered from manufacturers as possible so as to ensure that appropriate recommendations are made.     

Ovine obstetrics

Sir,—There has recently been some correspondence in the Veterinary Record (Vol. 69, No. 15 et seq.) on the subject of Ring Womb in sheep. Whether or not one should leave the ewes to lamb in 2 or 3 days or more, as suggested by Mr. Straiton, remains debatable and can be left to those who are willing to try. However, for those who prefer to take prompt action, I would like to describe a method I have adopted in my practice. r have found that caesarian section, which is recommended for this condition, is time-consuming and is done preferably at the surgery for best results. My method can be performed quickly in the field and the results are excellent, although it necessitates culling the ewe from the breeding flock. This is not realy a disadvantage, as the condition tends to recur and thus the ewe should be culled anyway.     

Oestrogen,Progesterone and Oxytocin Receptors and COX‐2 Expression in Endometrial Biopsy Samples from the Induction of Ovulation to Luteolysis in Llamas (Lama glama)

Endometrial expression of oestrogen (ERα), progesterone (PR) and oxytocin receptor (OR) and cyclooxygenase‐2 (COX‐2) was evaluated from the induction of ovulation to luteolysis in llamas. Ovarian activity was daily assessed by ultrasonography in five females. Ovulation was induced immediately after the detection of an ovulatory follicle by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left and right horns on day 0 and days 4, 8, 10 and 12 post‐GnRH. Blood samples were collected daily for progesterone and estradiol‐17β determinations by RIA. An immunohistochemical technique was used to study receptors population and COX‐2 expression which were then evaluated by two independent observers. The expression of ERα and PR was highest on day 0 in the luminal epithelium and stroma in association with high plasma estradiol‐17β concentrations. Thereafter, a decrease in ERα population was registered on day 4 and a new increase of its expression was observed between days 8 and 12 in those cell types. Conversely, PR population was gradually down‐regulated until its lowest expression was reached on day 10 post‐GnRH in the luminal epithelium. Content of OR was similar throughout the study in all cell types. The expression of COX‐2 was highest from day 8 to 12 post‐GnRH in the luminal epithelium, in relation to the time of maximal PGF_2α release. Both steroid receptors populations and COX‐2 expression were similar between horns. Meanwhile, OR expression was higher in the right than in the left uterine horn. In summary, this study showed that the loss of endometrium sensitivity to progesterone by days 8–10 post‐induction of ovulation and the concomitant increase of COX‐2 expression could play a key role in the mechanism of luteolysis and somehow be related to the short corpus luteum lifespan of llamas.     

Lateral and Vertical Variations of Soil Organic and Inorganic Carbon Content in Aridisols and Entisols of a Rangeland

Eurasian Soil Science - The influence of biological and physicochemical soil properties on the variations in soil organic and inorganic carbon (OC and IC) contents at the soil surface was studied....     

Effects of nitrogen fertilization and in-row seedpiece spacing on yield of three potato cultivars in Prince Edward Island

AC Novachip and NorWis were recently introduced as new potato (Solanum tuberosum L.) chip cultivars on Prince Edward Island (P.E.I.), Canada. Variability of nitrogen application and in-row seedpiece spacing, required for maximum yield, exist among cultivars presently grown in P.E.I. This study was conducted to determine the N rate and in-row seedpiece spacing required for optimum production of AC Novachip and NorWis compared to the standard cultivar Norchip, and also, to determine the effects of treatments and harvest dates on AC Novachip. Treatments included N applied at 90, 134, and 179 kg/ha with in-row seedpiece spacings at 20.3, 25.4 and 30.5 cm. In addition to the 120 day harvest for all three cultivars, treatments for AC Novachip were also harvested at 80 and 100 days after planting. For the 120 day harvest, yield of Canada No. 1 size tubers was higher for NorWis compared to Norchip while yields for AC Novachip and Norchip were similar. Nitrogen application had no effect on yields of AC Novachip. Similar to Norchip, increasing the rate of applied N more than 134 kg/ha resulted in little or no increase in yields of NorWis. Yields of Canada No. 1 size tubers decreased as in-row spacing increased for AC Novachip and were greater at 25.4 compared to 30.5 cm spacing for Norchip. In-row seed-piece spacing had no affect on Canada No. 1 size tuber yields of NorWis. Increasing the days to harvest increased the yields of AC Novachip. Nitrogen rate or in-row spacing had no effect on Canada No. 1 yields of AC Novachip at the 80 and 100 day harvests. The results of this study suggest that little gain in yield of Canada No. 1 size tubers for NorWis, similar to the standard cultivar Norchip, can be obtained with N application more than 134 kg/ha or for AC Novachip by increasing the rate of N from 90 to 179 kg/ha. Unlike NorWis yields of AC Novachip and NorWis are reduced when in-row seedpiece spacing is increased from 25.4 to 30.5 cm.