Clinical evaluation of detomidine-butorphanol-guaifenesin-ketamine as short term TIVA in Spiti ponies

Veterinarians working under remote field conditions are routinely presented with variety of surgical interventions in equines like castrations, management of wound, traumatic and congenital hernias and musculoskeletal disorders thus necessitating the use of general anaesthesia for management of these conditions. The present study was carried out to evaluate and recommend the suitable short term anaesthetic technique for Spiti ponies under field conditions. Seven clinically healthy male Spiti ponies presented for castration were evaluated for short term Total Intravenous Anaesthesia (TIVA) using detomidine (0.02 mg kg(-1)), butorphanol (0.01 mg kg(-1)), 5% guaifenesin (20 mg kg(-1)) and ketamine (2.0 mg kg(-1)). The studies conducted were open label trials and all the animals received same treatment. After proper tetanus prophylaxis and preanesthetic fasting, detomidine was administered intravenously. Subsequently at head down position the animals received butorphanol intravenously. Thereafter, guaifenesin was administered intravenously. As soon as the signs of ataxia developed, the induction of surgical anaesthesia was achieved by intravenous administration of ketamine hydrochloride. The onset of sedation was observed in 2.43 +/- 0.53 min following detomidine administration and the animals were ataxic in 1.43 +/- 0.43 min after butorphanol and guaifenesin administration when ketamine was injected. The ponies were in surgical plane of anaesthesia within 2.28 +/- 0.42 min following ketamine administration. During recovery the limb/head movement and sternal recumbency were attained in 18.71 +/- 1.98 and 26.14 +/- 1.62 min, respectively whereas standing ataxia and normal gait were seen at 29.42 +/- 3.21 and 71.14 +/- 4.74 min, respectively. There was excellent to good muscle relaxation. The surgical anaesthesia remained for 22.57 +/- 1.48 min. The recovery was smooth. Moderate to good suppression of palpebral and corneal reflexes were observed immediately after induction and during anaesthesia. The analgesia was excellent. A highly significant (p < 0.01) to significant (p < 0.05) decrease in respiration rate was observed after induction, during anaesthesia and after recovery. The mean SpO2 value in equines of this group was 76.50 +/- 4.14 and 83.33 +/- 4.18% after induction and during anaesthesia, respectively. Some of the blood biochemical parameters like plasma alanine amino transferase (ALT), total proteins and glucose showed significant increase without clinical consequence. It was concluded that detomidine (0.02 mg kg(-1)), butorphanol (0.01 mg kg(-1)), guaifenesin 5% (20 mg kg(-1)) and ketamine (2.0 mg kg(-1)) combination can safely be used for short term total intravenous anaesthesia in equines under field conditions where the monitoring facilities are meager.     

Effectiveness of Ethrel as a Male Gametocide in Pearl Millet and its Influence on Ergot

In field and greenhouse experiments Ethrel (2-chloroethyl phosphonic acid) was tested for its male garnetocidal effects on pearl millet (Pennisetum americanum) and its subsequent effects on ergot development. Application of Ethrel at 2000 ppm at late boot or early protogyny was the most effective for inducing male sterility in the hybrid, EJ 104. Female fertility in a male sterile line, however, was not affected by Ethrel treatment. Ethrel at 2030 ppm applied at ihe late boot stage resulted1 in partial paniele exsertion, and reduced plant height anc. panide length. In vitro Ethrel (2000 ppm) completely inhibned pollen germination but did not affect germination of conidia of Claviceps fusiformis, the causal agent of ergot of pearl millet. Ergot resistance or susceptibility in pearl millet lines was not affected., probably because Ethrel could not induce complete male sterility.     

Linkage studies in indica rice,Oryza sativa L.

Summary It has been found that long palea, an easily identifiable trait, is attributable to two duplicate recessive genes designated lp ₁ and lp ₂. A new character, uneven kernel has been described, the espression of which is controlled by two dominant complementary genes, Un _a and Un _b. Two dominant complementary genes, Cl _a and Cl _b have been found controlling clustered habit.One of the complementary genes for clustered habit, Cl _a was linked with one of the complementary genes for uneven kernel, Un _a with a c.o. value of 22.1 per cent. This linkage has been assigned to I linkage group in indica rice.The long glume gene g showed 22.2 per cent linkage with oed rice gene, Rc. It was further found linked with one of the complementary genes for uneven kernel, Un _b (17.8 per cent) and one of the duplicate recessive genes for long palea, lp ₁ (30.2 per cent). The cross-over value between Ub _b and lp ₁ was 11.5 per cent. The gene order appeared to be lp ₁-Un _b-g-Rc. These linked genes have tentatively been assigned to IV linkage group in indica rice.     

Bioremediation of Pulp and Paper Mill Effluent by Tannic Acid Degrading Enterobacter sp.

Wastewaters from pulp and paper mills are highly toxic and around 250 xenobiotic compounds have been reported in the effluents. Tannic acid degrading bacterium, Enterobacter sp. was isolated from soil by tannic acid enrichment. This isolate was used for bioremediation of pulp and paper mill effluents. Parameters like temperature, agitation, inoculum size and treatment duration were optimized by using Qualiteck-4 software. Reduction in lignin 73% and colour up to 82% was also observed. Encouraging results were observed is reduction of COD, BOD with 16-h retention time in batch culture.     

Assessment of soil quality monitoring indicators under long term rice cultivation in hot arid Ghaggar-flood plains of India

ABSTRACT

The evaluation of soil quality is essential in monitoring the long term effects of rice cultivation. Present study investigated the effects of long term rice cultivation on soil properties and organic C pools and identified indicators for monitoring soil quality in Ghaggar-flood plains of hot arid India. Soil samples were collected from fields with 0, 10, 20, 30 and 40 years of rice cultivation. The study revealed that electrical conductivity (EC) and exchangeable sodium percentage (ESP) increased after 30–40 years of rice cultivation. Available nutrients increased with increasing years of rice cultivation. The organic carbon pools namely, total organic carbon (TOC), Walkley Black carbon (WBC) and particulate organic carbon (POC) were increased above 50% in 20 and above years of rice cultivation. The TOC and POC were increased by 40.6 to 132.4% and 31.7% to 104.8% in 10 to 40 years of rice cultivation. Cation exchange capacity, WBC, ESP and CaCO₃ could serve as soil monitoring indicators of long term rice cultivation in arid region. The findings clearly indicated that long term rice cultivation could aggravate soil salinity and have negative impact on soil quality in arid environment.     

Diversity of entomopathogenic bacteria associated with the white grub, Brahmina coriacea

A survey of potato fields located in the south-eastern region of Himachal Pradesh (India) was carried out in order to find out the natural pathogens infecting the white grub, Brahmina coriacea. About 88 % population of the infected grubs were found to exhibit symptoms of natural bacterial infection during the years 2007–2008. Hence, we attempted to isolate and characterize the most potent bacteria for the management of B. coriacea and tested their insecticidal activity. In this study, ten different bacterial isolates belonging to genera Bacillus, Psychrobacter, Paracoccus, Paenibacillus, Mycobacterium, Staphylococcus and Novosphingobium were isolated from B. coriacea. Bacterial species were identified based on morphology, biochemical tests and homologies of 16S rRNA gene sequences. Pathogenicity tests for all isolated bacteria at 1.0 × 10⁸ cfu/ml of broth were performed on late first instar grubs. Among the bacteria tested, Bacillus cereus induced highest mortality level of 51.85 % within 7 days of treatment followed by Psychrobacter pulmonis (33.33 %), Bacillus psychrodurans (25.93 %), Bacillus pumilus (25.93 %), Paenibacillus tylopili (22.22 %) and Novosphingobium capsulatum (18.52 %). Mortality levels were further increased up to 100 % by B. cereus followed by 88.89 % by P. pulmonis after 30 days of treatment. Our results indicate that B. cereus, P. pulmonis, B. psychrodurans, B. pumilus, P. tylopili and N. capsulatum may be valuable biological control agents for white grubs, B. coriacea.     

Taguchi analysis of fabric shrinkage

This article reports on the effects of fabric constructional factors such as fabric sett, warp tension, back rest position, and pick insertion rate, on the warp-way and weft-way shrinkages of a three-up-one-down twill woven fabric using a Taguchi design of experiments. It was observed that the fabric shrinkage in the warp direction was mostly affected by the change in the number of picks per inch, followed by warp tension, number of ends per inch, number of picks per minute, and back rest position, respectively. The fabric shrinkage in the weft direction was found to be mostly affected by the change in number of ends per inch, followed by number of picks per inch, back rest position, number of picks per minute, respectively and least affected by warp tension. It was observed that the aforesaid five factors were able to explain 99.02 % and 82.81 % of the total variation of fabric shrinkage in the warp and weft directions, respectively.