“互联网+”视域下农业众筹风险防范机制构建研究

农业众筹作为依托于互联网金融的新型融资工具,是一种新型的服务三农的融资模式,有助于推进农业供给侧结构性改革,为农村经济发展注入新活力。传统的风险防范机制已经不能适应农业众筹在模式、监管等方面发生的变化,为弥补传统农业众筹风险防范的局限,在分析农业众筹运作模式、风险来源的基础上,构建了农业众筹风险防范机制,并在此基础上提出了合理的风险防范措施,以提高农业众筹风险防范的实时化、智能化水平。     

青天葵总黄酮抗氧化及抑菌活性

以青天葵为试材,乙醇为溶剂,对青天葵进行总黄酮提取;采用清除羟自由基、DPPH自由基、超氧阴离子自由基和测定总还原能力的方法来评价青天葵总黄酮抗氧化活性;并通过测定其对6种试验菌的最低抑菌浓度来考察其抑菌活性。结果表明:青天葵黄酮提取液对羟自由基的清除优于同浓度的抗坏血酸溶液,清除DPPH自由基的IC50是17.4μg·mL~(-1),清除超氧阴离子自由基的IC50是95.5μg·mL~(-1),还原能力稍弱于抗坏血酸;对白色念珠球菌、铜绿假单胞菌和肺炎克雷伯氏菌的抑制作用较强,最低抑菌浓度均为0.25mg·mL~(-1)。     

基于自压滴灌工程过滤装置尺寸的确定及应用

由于新疆牧业灌区无电供应,水源无法过滤,实现滴灌工程难,目前采用地面灌溉。地形坡度大、灌水量多、大量水土流失。灌区下游排水不畅,洼地沼泽化面积剧增,地下水位上升导致土壤盐碱化,弃田面积逐渐扩大、种植面积萎缩。为了解决上述问题,充分利用地形落差,研制了自压滴灌工程过滤装置。同时渠道进水流量、调水池的调节流量、集水桶流量及系统流量之间建立了平衡关系。用实例说明不同地形落差与自压滴灌工程系统流量及过滤装置尺寸的计算过程。解决了自压滴灌工程水源的过滤、排沙、冲沙及自行冲洗等问题。为无电牧业灌区实现节水灌溉技术提供依据。     

河西灌区施钾对啤酒大麦产量及品质的影响

采用土壤养分状况系统研究法（ASI）,又称土壤养分状况综合评价法,研究河西灌区施钾对啤酒大麦产量、品质的影响,并确定甘肃河西灌区合理经济的施钾量。结果表明固定N、P及微量元素等养分后,与不施钾比较。施钾的啤酒大麦的产量增加279～1325kg／hm^2,增幅5．9％～27．9％,千粒重提高0．1～2．8g;钾水平在0～150kg／hm^2范围内,随着施钾量的增加,产量、经济效益、千粒重也随之增加,籽粒粗蛋白含量降低。说明施钾提高了啤酒大麦的产量与经济效益,改善并保证了啤酒大麦的品质,合理经济的钾用量为150kg／hm^2.     

高效液相色谱法测定胡椒叶片中6-BA与GA3激素含量

采用Waters nova-pak C18色谱柱(3.9 cm×15 cm,5μm),87双波长紫外检测器,以甲醇∶乙腈∶磷酸缓冲液(pH 3.5)=15∶15∶70(体积比)为流动相,流速0.8 mL/min,柱温35℃,检测波长210 nm的条件下同时分离测定胡椒叶片中GA3、6-BA植物激素。GA3和6-BA各峰分离效果理想,加标回收率分别为90.80%和88.78%。     

Relationship between the ultrasonographic findings of suspected superficial digital flexor tendon injury and the prevalence of subsequent severe superficial digital flexor tendon injuries in Thoroughbred horses: a retrospective study

The onset of severe injury to the superficial digital flexor tendon (SDFT) is extremely difficult to predict from slight changes in ultrasonographic findings in cases with no apparent clinical signs. This study investigated the relationship between an increased cross-sectional area (CSA) or edema in the subcutaneous tissue around the tendon and the subsequent onset of severe SDFT injury in Thoroughbred racehorses. Horses were classified into three groups based on ultrasound diagnosis (USD) findings: Group A included cases with enlarged tendons; Group B included cases with tendons of normal size but with prominent edema in the peritendinous tissue; and Group C (control group) included cases with no abnormal USD findings. The incidence of subsequent severe tendon injury was significantly higher in the horses in Groups A (25.7%, 28/101) and B (28.3%, 65/212) than in those in Group C (4.9%, 2/41). There were no significant differences in the median period and the median number of races from the first examination to the subsequent tendon injury between Groups A (140 days, 1 race) and B (120 days, 1 race). The results of this study revealed that horses with increased CSA and peritendinous edema are likely to suffer a subsequent severe tendon injury. Also, these two USD findings, i.e., increased CSA and peritendinous edema, indicate the risk of onset of severe SDFT injury.     

Aurasperone A Inhibits SARS CoV-2 In Vitro: An Integrated In Vitro and In Silico Study

Several natural products recovered from a marine-derived Aspergillus niger were tested for their inhibitory activity against SARS CoV-2 in vitro. Aurasperone A (3) was found to inhibit SARS CoV-2 efficiently (IC₅₀ = 12.25 µM) with comparable activity with the positive control remdesivir (IC₅₀ = 10.11 µM). Aurasperone A exerted minimal cytotoxicity on Vero E6 cells (CC₅₀ = 32.36 mM, SI = 2641.5) and it was found to be much safer than remdesivir (CC₅₀ = 415.22 µM, SI = 41.07). To putatively highlight its molecular target, aurasperone A was subjected to molecular docking against several key-viral protein targets followed by a series of molecular dynamics-based in silico experiments that suggested M^pro to be its primary viral protein target. More potent anti-SARS CoV-2 M^pro inhibitors can be developed according to our findings presented in the present investigation.     

伪狂犬病病毒在NF-κB家族p65基因敲除细胞系的复制规律研究

试验旨在研究伪狂犬病病毒（PRV）在NF-κB家族p65基因敲除细胞系中的复制规律。利用慢病毒介导的CRISPR/Cas9基因定点修饰技术构建猪肺泡巨噬细胞（3D4/21）p65基因稳定敲除细胞系。通过构建p65-sgRNA重组质粒,转染至HEK293T/17细胞,收取慢病毒,感染3D4/21细胞后利用嘌呤霉素筛选获得多克隆细胞系,T7核酸酶检测敲除效率,再通过有限稀释法获得3D4/21-p65^-/-的稳定细胞系。CCK-8试剂盒检测3D4/21细胞中敲除p65基因后对细胞增殖的影响;流式细胞术检测PRV-GFP感染3D4/21及3D4/21-p65^-/-细胞后病毒增殖的差异;实时定量PCR检测PRV感染3D4/21及3D4/21-p65^-/-细胞后PRV gB、TK基因mRNA表达水平及PRV感染细胞诱导的IL-1β和IL-6基因mRNA水平表达的变化;Western blotting检测PRV-QXX感染3D4/21及3D4/21-p65^-/-细胞后PRV gB、gE蛋白的表达;滴度测定检测PRV-QXX感染3D4/21及3D4/21-p65^-/-细胞后子代病毒滴度。结果表明,sgRNA2和sgRNA3的基因编辑效率较高,对其进行克隆化培养进而获得敲除p65基因的稳定表达细胞系;CCK-8试剂盒检测细胞活力表明,p65基因敲除对细胞活力无影响;流式细胞仪检测表明,同一时间点PRV-GFP在3D4/21-p65^-/-中的增殖显著高于对照细胞;实时荧光定量PCR表明在3D4/21细胞中敲除p65基因促进了PRV gB、TK基因的mRNA表达水平,而抑制了IL-1β、IL-6基因的mRNA表达;Western blotting结果表明,在3D4/21细胞中敲除p65基因促进了PRV gB、gE蛋白的表达;滴度测定结果表明,同一时间点PRV-QXX在3D4/21-p65^-/-细胞中子代病毒的复制显著高于对照细胞。以上结果均表明,p65基因敲除可促进PRV在3D4/21细胞中复制。     

添加番茄渣单贮和混贮对奶牛生产性能和经济效益的影响研究

研究番茄渣单贮或混贮替代部分全株玉米青贮对奶牛生产性能和经济效益的影响,为今后在奶牛科学养殖中开发和利用非常规饲料资源提供理论依据和技术支持。试验选择体重、泌乳期、胎次和产奶量相近且健康无疾病的30头泌乳前中期荷斯坦奶牛于内蒙古巴彦淖尔市临河区双河镇嘉益奶牛养殖合作社进行为期50 d的饲养试验。结果表明,番茄渣经过单贮或混贮(分别与梨渣、玉米秸秆、小麦秸秆混贮)后用于奶牛日粮中替代部分全株玉米青贮,可有效的降低玉米青贮的使用,降低生产成本,显著提高乳蛋白率(P<0.05)、乳脂率(P<0.05)和奶牛产奶量(P<0.05),提高养殖效益。番茄渣单贮或混贮可提高奶牛生产性能和经济效益,并可作为一种非常规饲料资源在奶牛业中进行推广应用。     

从地下的角度分析膜下滴灌棉花发生早衰的可能机制

在膜下滴灌条件下,棉花早衰问题严重。在对国内外关于棉花早衰研究成果及膜下滴灌条件特殊根区微环境深入分析的基础上,提出了膜下滴灌棉花早衰原因的合理假设,认为不合理的根系构型及其高蕾铃负荷是造成膜下滴灌棉花早衰的根本原因：膜下滴灌条件下棉花根系生长和构型分布发生的明显变化不利于棉花根系吸收土壤深层的水分和养分,抗逆性减弱,对环境的改变无法做出及时的反应,加之覆膜增温及优越的水肥供应,棉花地上部生长良好,蕾铃负担增加,一旦遇到逆境条件,在高蕾铃负荷前提下,即使对根系养分吸收功能最轻微的损害或者暂时的养分供应短缺均有可能造成对地上部养分供应的不足并发生早衰。因此,如何通过调控构建与地上部生长更为匹配的、构型分布更加合理的、抗逆性更强的棉花根系就成为解决膜下滴灌棉花早衰问题的关键。