Astaxanthin protects against oxidative stress and calcium-induced porcine lens protein degradation

Astaxanthin (ASTX), a carotenoid with potent antioxidant properties, exists naturally in various plants, algae, and seafoods. In this study, we investigated the in vitro ability of ASTX to protect porcine lens crystallins from oxidative damage by iron-mediated hydroxyl radicals or by calcium ion-activated protease (calpain), in addition to the possible underlying biochemical mechanisms. ASTX (1 mM) was capable of protecting lens crystallins from being oxidized, as measured by changes in tryptophan fluorescence, in the presence of a Fenton reaction solution containing 0.2 mM Fe2+ and 2 mM H2O2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis demonstrated that beta(high)-crystallin was the most vulnerable protein under these conditions of free radical exposure. The proteolysis of lens crystallins induced by calcium ion-activated calpain was also inhibited by ASTX (0.03-1 mM) as determined by daily measurement of the light-scattering intensity at 405 nm for five consecutive days. ASTX at 1 mM was as potent as a concentration of 0.1 mM calpain inhibitor E64 in protecting the oxidative damage/hydrolysis of porcine crystallins. At a concentration of 1 mM, ASTX provided better protection than the endogenous antioxidant glutathione in terms of suppressing calcium-induced turbidity of lens proteins. Thin-layer chromatography analysis indicated that ASTX interacted with calcium ions to form complexes, which we believe interfere with the hydrolysis of lens crystallins by calcium-activated calpain. This in vitro study shows that ASTX is capable of protecting porcine lens proteins from oxidative insults and degradation by calcium-induced calpain.     

Characterization of a novel coronavirus associated with severe acute respiratory syndrome

In March 2003, a novel coronavirus (SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS). The sequence of the complete genome of SARS-CoV was determined, and the initial characterization of the viral genome is presented in this report. The genome of SARS-CoV is 29,727 nucleotides in length and has 11 open reading frames, and its genome organization is similar to that of other coronaviruses. Phylogenetic analyses and sequence comparisons showed that SARS-CoV is not closely related to any of the previously characterized coronaviruses.     

An overview of salmon trout aquaculture in Finland

Abstract

In this case study, we describe the evolution of Finnish salmon trout aquaculture; how salmon trout farming grew as a vital industry, how Finland became the world's leading producer of salmon trout, and how the opening of markets to international competition led to a decline of the industry. The focus is on the continuous interaction between the changing market situation and production decisions. The study reveals the impact of national environmental policy on the competitiveness of the industry. At the moment, the industry is undergoing major structural changes; production has moved to neighbouring countries, and the value chain is concentrated. In future, closer interaction and co‐operation between the actors along the entire fish value chain will be the key factors for success.     

Golden Grey Mullet (Liza aurata) Alkaline Proteases: Biochemical Characterization,Application in the Shrimp Wastes Deproteinization,Laundry Commercial Detergents,and Preparation of Antioxidant Protein Hydrolysate

Digestive alkaline proteinases from golden grey mullet (Liza aurata) were extracted and characterized. The crude alkaline protease showed optimum activity at pH 8.0 and 60°C, and it was highly stable over a wide range of pH from 4.0 to 10.0, retaining more than 80% activity after incubation for 1 h at 4°C. The alkaline proteases showed extreme stability toward nonionic and anionic surfactants after preincubation for 1 h at 25°C and relative stability toward oxidizing agents. Additionally, the crude enzyme showed excellent stability and compatibility with various solid and liquid detergents. Further, proteases from golden grey mullet viscera were found to be effective in the deproteinization of shrimp wastes. The protein removal after 3 h at 45°C with an enzyme/substrate (E/S) ratio of 10 U/mg protein was about 76%. The golden grey mullet proteases were also shown to be efficient in the production of antioxidant protein hydrolysate.     

Evaluation of the effect of ranitidine on gastroduodenal contractile activity and gastric emptying in horses

Objective-To determine the effect of ranitidine on gastric emptying in horses. Animals-11 adult horses. Procedures-In vitro, isolated muscle strips from the pyloric antrum and duodenum of 5 horses were suspended in baths and attached to isometric force transducers. Once stable spontaneous contractions were observed, ranitidine or diluent was added at cumulative increasing concentrations. Isometric stress responses were compared. In vivo, 6 horses were assigned to a group in a prospective randomized crossover study design with a wash-out period of 2 weeks between trials. Ranitidine (2.2 mg/kg) or saline (0.9% NaCl) solution was administered IV, and 15 minutes later, acetaminophen (20 mg/kg), diluted in 400 mL of water, was administered via nasogastric tube to evaluate the liquid phase of gastric emptying. Serum acetaminophen concentration was measured at several time points for 3 hours by use of liquid chromatography tandem mass spectrometry. Frequency of defecation was recorded during the 3 hours of the study. Results-Ranitidine increased the contractile activity of the pyloric antrum smooth muscle at a concentration of 10(4) M. No significant effect of ranitidine on plasma kinetics of acetaminophen was identified. Frequency of defecation did not differ between groups. Conclusions and Clinical Relevance-Ranitidine did increase gastric motility in vitro, but no effect on liquid phase gastric emptying was identified in healthy horses by use of the acetaminophen absorption model. Results do not support the use of ranitidine to promote gastric emptying.     

Climate-induced overwintering challenges for wheat and rye in northern agriculture

Abstract

Winters are typically harsh in the northernmost agricultural areas of Europe, and winter rye (Secale cereale L.) and wheat (Triticum aestivum L.) are the only winter grain crops that can be grown. However, climate change is projected to result in milder winters, which may enable cultivation of winter crops to a greater extent in the future than is possible today. In this study we aimed at identifying main temperature, precipitation events and characteristics that have resulted in past poor overwintering of rye and wheat in their current production areas in Finland. Using long-term (1970–2006), multi-location datasets, we compared our findings with the projected major changes attributable to climate change. Mixed models were used to estimate mutually comparable overwintering damage to all experiments and logistic regression was used to determine whether climatic parameters are related to high levels of overwintering damage. Severity of overwintering damage, and associated yield penalties, fluctuate considerably on a year-to-year basis and no consistent reduction in variability was recorded during the study period. Particularly for wheat, severity of winter damage in any one year was associated negatively with area sown in the following year. There was no evidence of consistent genetic improvements in winter hardiness, but rye was more winter hardy than wheat. Current risks associated with rye production related to low temperatures could be alleviated in the future, although overwintering damage currently enhanced by high autumn precipitation could increase due to climate change. For wheat, fluctuating conditions hampered overwintering, which may be an even harder challenge in future when weather variation is projected to increase and extreme weather events are projected to become more common.     

Rheological behavior of WPI dispersion as a function of pH and protein concentration

Physical and flow properties of proteins can provide information necessary for the optimal design of unit processes and quality control of the manufacturing process and final products. Therefore, the purpose of this investigation was to characterize the rheological behavior of a whey protein isolate (WPI) (BiPRO) dispersion as a function of pH and protein concentration. A rotational viscometer was used to determine the apparent viscosity, shear rate, and shear stress of WPI dispersions. Both the consistency index (k) and the flow behavior index (n) were sensitive to changes in pH and protein concentration. Mathematical relations obtained from experimental values of k and n allowed the determination of a model for apparent viscosity (eta) of WPI dispersions as a function of pH and protein concentration. At 5 and 10% BiPRO, whatever the pH, the rheological behavior appeared to be a newtonian fluid, while at 20% BiPRO, the rheological behavior appeared to be a nonnewtonian pseudoplastic fluid. Furthermore, at 20% Bipro, the apparent viscosity presented an increase in viscosity from 5.6 to 5.4, followed by a decrease from pH 5.4 to 5.0 at all shear rates. The highest viscosity was obtained at 20% pH 5.4, with an approximate value of 0.25 Pa.s, 10 times higher than the one obtained at 5 and 10% BiPRO.