我国部分地区送检样品3种常见猪病血清学和病原学检测

为了解我国部分地区猪瘟、猪繁殖与呼吸综合征和猪伪狂犬病3种常见疫病的流行情况,采用ELISA、聚合酶链式反应（PCR）和反转录PCR（RT-PCR）等方法,对2019年11个省（自治区）172个猪场送检的3 550份猪血清样品进行猪瘟病毒（CSFV）、猪繁殖与呼吸综合征病毒（PRRSV）及伪狂犬病野毒（PRV-gE）抗体检测,对9个省（自治区）72个猪场送检的371份病料进行抗原检测。结果显示：CSFV、PRRSV、PRV-gE抗体阳性率分别为82.75%、84.08%、31.86%,抗原阳性率分别为2.62%、26.15%、1.67%。保育猪群CSFV抗体平均阻断率最低,为44.62%,抗体整齐度差,CV为58.26%,且CSFV抗原主要在哺乳仔猪和保育猪病料中被检出,检出率分别为3.57%、3.00%;除育肥猪群外,其他各阶段猪群的PRRSV抗体S/P平均值为1.0~2.0,抗体整齐度整体较差,且平均抗原阳性率较高（26.15%）,其中保育阶段猪群病原检出率最高（37.68%）;育肥猪、母猪、公猪的PRV-gE抗体阳性率分别为36.82%、35.31%、15.65%,病原主要从死胎/木乃伊胎和哺乳仔猪病料中被检出,检出率分别为8.70%和2.99%。结果表明,PRRSV对我国猪场不同阶段猪群均有较大威胁;保育猪群CSFV抗体水平不理想,暴发疫情风险较高;公猪、母猪及育肥猪群普遍存在PRV感染,尤其是母猪。提议各猪场采取免疫、监测、净化等综合措施,加强这3类常见猪群疫病的防控。     

Isolation, expansion, and differentiation of goat adipose-derived stem cells

A goat adipose-derived stem cell (ADSC) line was established and compared to a rat line. Goat ADSC cells had normal diploidy after subculture. Proliferation of goat ADSCs was faster than rat cells in the same conditions. Both rat and goat ADSCs stained positively for vimentin, CD49d, CD44 and CD13, but stained negatively for CD34 and CD106. Bone nodules were apparent, and alizarin staining was positive after osteogenic induction. Cells expressing osteocalcin were positive by alkaline phosphatase (ALP) staining. After osteogenic induction, ossification nodules of goat ADSCs were larger than in rats, with dense ALP staining. Adipogenic induction resulting in lipid droplets and peroxisome proliferator-activated receptor (PPARγ2) expression were observed. Cartilage lacunae were formed and COL2A1 was expressed. More cartilage lacunae with better morphology were seen following differentiation of goat ADSC's using the hang-drop method. For goat ADSCs, results with both adherent-induced and hanging-drop induced cultures were better than for three-dimensional cultures.     

猪源Cathelicidins抗菌肽家族的研究进展

Cathelicidins是一类在哺乳动物中发现的含有保守的cathelin区域的抗菌肽,是宿主防御系统的重要组成部分,具有广谱且强大的抗菌活性。文中综述了猪源Cathelicidins抗菌肽家族的基因组成、结构、抗菌活性、作用机制及应用前景。     

东北豹保护对策研究

通过概述世界上东北豹的种群数量特征和分布状况,分析了东北豹保护面临的各种威胁和问题,如人类过度干扰、乱砍滥伐、乱捕滥猎、近亲繁殖以及人类的开发建设等活动对栖息地造成的严重破坏,使栖息地呈片段化、破碎化发展趋势,直接威胁东北豹种群的生存和繁衍。中国对东北豹的保护没有足够的重视,还没有建立专门的东北豹自然保护区,而俄罗斯对于东北豹的保护非常重视,不但建立了专门的东北豹自然保护区,还完善了东北豹的各项保护措施。最后,针对上述情况,提出了东北豹栖息地保护、猎物种群恢复、东北豹的重引入、建立东北豹跨界保护区等相应的保护对策。     

中药饲料添加剂对仔猪生长性能和免疫功能的影响

通过在基础日粮中添加2种不同精制中药饲料添加剂,研究其对仔猪生长性能和免疫功能的影响.选用45头平均体重为10kg左右的杜长大三元杂交断奶仔猪进行试验,试验分3组,分别为中药Ⅰ组(中药添加剂Ⅰ),中药Ⅱ组(中药添加剂Ⅱ),对照组(抗生素、化学合成药物组),全程试验分保育Ⅰ阶段(23天)和保育Ⅱ阶段(30天),在每一阶段末期,分别采集血样测定血清免疫球蛋白(IgG、IgM、IgA)、补体(C3、C4)和白细胞介素-2(IL-2)的含量.结果如下:中药Ⅰ组、中药Ⅱ组平均体增重和日增重提高,料重比及腹泻率下降;血清免疫球蛋白、补体和白细胞介素-2的含量明显升高.以上结果表明,中药饲料添加剂Ⅰ和Ⅱ能有效地防止保育阶段的仔猪发生腹泻,改善生长性能,并能促进免疫球蛋白(IgA、IgM、IgG)、补体(C3、C4)和白细胞介素-2的生成,提高机体免疫功能.     

狗年侃犬

狗年侃犬,犬即是狗,狗即是犬。就字而言,狗是口头语为多,犬是书面语为多。犬与人类的交流与融合,源远流长,这是一个说不完、道不尽的话题。犬的文化也是人们津津乐道的话题,也是一个国家一个民族的文化。我国养犬的历史有几千年,对犬也说法众多,褒贬不一。狗、犬从生物科学来讲都是表述同样的动物,但从中国人文化的内含却有很大的差异。中国人对狗这个字的理解大都含有贬意,民间通俗用语,比较粗糙。对犬的理解似乎有些褒奖与赞美之意,是学术用语,有文化内涵。狗常常是用来形容不好的人或事,是没有教养与没有学问的,人类对它是要进行限制管理…     

Building phenotype networks to improve QTL detection: a comparative analysis of fatty acid and fat traits in pigs

Models in QTL mapping can be improved by considering all potential variables, i.e. we can use remaining traits other than the trait under study as potential predictors. QTL mapping is often conducted by correcting for a few fixed effects or covariates (e.g. sex, age), although many traits with potential causal relationships between them are recorded. In this work, we evaluate by simulation several procedures to identify optimum models in QTL scans: forward selection, undirected dependency graph and QTL-directed dependency graph (QDG). The latter, QDG, performed better in terms of power and false discovery rate and was applied to fatty acid (FA) composition and fat deposition traits in two pig F2 crosses from China and Spain. Compared with the typical QTL mapping, QDG approach revealed several new QTL. To the contrary, several FA QTL on chromosome 4 (e.g. Palmitic, C16:0; Stearic, C18:0) detected by typical mapping vanished after adjusting for phenotypic covariates in QDG mapping. This suggests that the QTL detected in typical mapping could be indirect. When a QTL is supported by both approaches, there is an increased confidence that the QTL have a primary effect on the corresponding trait. An example is a QTL for C16:1 on chromosome 8. In conclusion, mapping QTL based on causal phenotypic networks can increase power and help to make more biologically sound hypothesis on the genetic architecture of complex traits.     

冷冻干燥法制备速溶植酸酶的研究

采用毕赤酵母发酵制备酶液,膜超滤浓缩、硫酸铵盐析法沉淀酶蛋白,结合冷冻干燥法制得速溶型植酸酶。结果表明,对酶活力达19 500U/ml的发酵液进行浓缩后,以70%饱和度的硫酸铵进行盐析,分离后的酶蛋白经过冷冻干燥得到活力为500 000U/g的植酸酶,速溶性好,溶解后清亮透明。酶的冻干粉50℃保存90d内酶活力仍能维持96%以上的活力。为高酶活速溶植酸酶的工业化生产提供了一条可行的思路。     

The phylogeographic system survey of native sheep breeds in the eastern and southern Central Asia

The genetic diversity and phylogenetic survey of native sheep breeds in the eastern and southern Central Asia were assessed in the present study. The clustering, principal components, structure and F statistics all demonstrate that the native sheep breeds in these regions be classified into two genetic groups: Mongolia‐Tibetan sheep group and South‐Southeast Asia sheep group. The Mongolia sheep group and the Tibetan sheep group had a certain degree of gene communication from the ancient times. In the present study we demonstrated that the Chinese native sheep populations belonged to Mongolia‐Tibetan sheep group. However, the relationships among the sheep populations in Mongolia sheep group in China were not closely related to the geographical distance among sheep populations.     

制备奶牛子宫内膜炎中药栓剂的优选方案研究

以中药制剂栓的色泽、不溶物、融变时限为标准,运用正交试验的方法优选治疗奶牛子宫内膜炎中药栓的制备方案.结果优选出的方案为聚乙二醇(PEG)1500 5g、PEG6000 25g,中药混合提取物6g,此方案稳定可行,并且由此制得的中药栓各项指标均符合药典规定.