Use of comparative proteomics to identify the effects of creatine pyruvate on lipid and protein metabolism in broiler chickens

Four hundred male chickens were selected to study the effects of pyruvate (Pyr), creatine pyruvate (CrPyr) and creatine (Cr) on the expression of hepatic mitochondrial and cytoplasm proteins associated with lipid and protein metabolism. Mitochondrial purification was accomplished using the two-step differential centrifugation and density gradient method, and the activities of organelle-specific marker enzymes were determined to assess the purity of the mitochondria. Proteins were extracted and fractionated by two-dimensional electrophoresis and the differential protein spots were assessed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. CrPyr reduced fatty acid accumulation by down-regulating adipose differentiation-related protein, inhibited ATP synthase expression, and reduced cholesteryl ester transfer protein (CETP) expression, thus reducing the levels of high density lipoprotein and triglycerol (TG) levels (thereby lowering fat and cholesterol deposition). CrPyr increased the expression of eukaryotic translation initiation factor (eIF) 2B, calreticulin (CRT) and eIF3a, thus promoting protein synthesis. CrPyr up-regulated the expression of fatty acid-binding proteins, CETP and apolipoprotein A-IV in cytoplasmic extracts, and these proteins accelerated the decomposition of fatty acids and TG, thus reducing fat deposition. In conclusion, CrPyr plays an important role in lipolysis and protein synthesis, and this effect was more pronounced than was the effect of Pyr and Cr.     

频频变脸的中国饲料营销(1)

大饲料集团卖的啥20世纪80年代末和90年代初,这一段时间内中国产生的暴发户之多,真可谓是空前绝后。我国的饲料业就是在这个时候萌芽的,由于饲料行业科技含量低,投资门槛可高可低,营销简单易行,设备可繁可简,最简单时可简单到一把铁锹……一夜之间,一些民企经营高手们逢时而起,像天女散花似的,把饲料厂散遍大江南北,长城内外,造就了不少下辖相当数量子公司的饲料集团公司。在四川,首先是希望集团的崛起。刘氏四兄弟中老三——刘永美(陈育新)是学畜牧专业的,他从养鹌鹑、孵化小鸡开始,然后到为养殖户着想,提供长得快、不生病的饲料,就这样不…     

禽流感(H9)疫苗免疫间隔期探讨

通过在不同日龄对海兰褐蛋鸡进行禽流感(H9)灭活疫苗的免疫,探讨禽流感灭活疫苗的免疫间隔期。结果表明,开产以后不管防疫2次、3次、4次禽流感灭活疫苗,抗体水平均在6log2以上,且鸡的日龄越大,免疫次数越少,免疫抗体持续期越长。因此,建议从育成到鸡群淘汰的饲养全程中,四次时机恰当、搭配合理的禽流感(H9)疫苗免疫基本上能使得95%以上产蛋期抗体维持于7log2以上。     

奶牛环形泰勒焦虫病诊治报告

２０００年６月上旬至７月下旬，我县营子、七贤等乡镇发生一种呈地方性流行或散发的急性奶牛疾病，以高热稽留、体表淋巴结肿大、贫血为主要特征。根据流行病学、临床症状、剖检变化、实验室诊断，确诊为奶牛环形泰勒焦虫（Ｔ．ａｎｎｕｌａｔａ）病，现报告如下：１流行病学从６月上旬开始陆续发病，发病率达２５％，其中死亡２头。该病以一胎母牛多发。经调查，多为带犊由外地购进，本地饲喂时间不足一年。检查牛体，能发现吸饱血或未吸饱血的豆粒大或米粒大牛蜱。２临床症状病牛初期体温上升高达４１℃，稽留热。肩前和鼠蹊淋巴结肿大，…     

由训练记录知美军军犬训练——《美军军犬作战条令》解读系列二

美军军犬训练十分注重过程跟踪和数据积累,每头军犬都建立了详实的训练使用档案,从其出生到死亡或退役,每天都记录军犬的训练使用科目、时间和饮食状况,并填写训练存在的问题和改进措施,每周至少安排24小时执行非训练任务,每月进行一次甚至两次体检。每头美军军犬训练使用记录都包括两类：一类是巡逻训练使用记录;     

Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus

Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found (₁VCKKITEHERCHVNI₁₅), (₂₀AFFGVMPRGTTPRAPVR₃₆), (₄₆RRGLETGWAYTHQGGI₆₁), (₂₈₁EGDMATGITYASYGYFC₂₉₇), (₄₂₆YSGEDPANLRVVTSQSPYVVVATNAIESGV₄₅₅) and (₄₈₁FIVTGLKRMAVTVGEQA₄₉₇) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody–antigen reaction of the non-structural proteins and may aid in the clinical application as well.     

利用BRILL饲料配方软件制定最佳经济效益配方

从事饲料配方的人员都知道,准确的原料数据和适当的饲养标准是饲料配方技术的关键所在。现在众多饲料企业已经注意到,并开始对一些主要原料的重要数据进行化验,如豆粕、鱼粉等的主要氨基酸指标。有经验的专业营养师都知道中国的饲料原料营养成分变化非常大,玉米、豆粕在不同产地不一样,甚至在同一产地变化也很大。所以,要获得效果好的饲料配方,首先要准确掌握原料数据。多数饲料配方是以NRC或品种饲养手册的饲养标准为基础,而配方师经常遇到的问题是能量达不到要求。由于能量是决定配方成本的首要因素,又是其他营养成分如氨基酸参与体内代…     

饲养过渡期奶牛的营养策略

奶牛产犊前后间隔的六周过渡期是一个非常关键的时段。此阶段不好的饲养管理会造成产奶量下降、繁殖效率的低下和增加代谢疾病等一系列问题，这不仅增加每千克牛奶的饲料成本而且也会降低牧场的整体营业收入。对于一个奶牛场而言，为了实现生产利润最大化就必须要求营养师、兽医师和牧场管理者达成共识，紧密配合共同来制定一个可行的饲养管理策略，以便帮助奶牛平稳地渡过产犊前后这个关键时期，以最大限度地发挥出奶牛的生产性能。     

野生动物旅游产品现状初探

野生动物旅游产品在总体的旅游产品中占有很大的市场份额,也有越来越多的旅游爱好者对此拥有购买动机与欲望。根据目前旅游市场现状,本文对野生动物旅游产品的种类进行了归类,提出了野生动物旅游产品可分为观赏型、参与型、工艺品型、食用型和药用型等5种表现形式。结合中国在野生动物旅游产品方面的开发现状,从生态保护、增强体验性与真实性、社区共管、科普教育、法制建设和科学投资方面提出了合理开发的建议。     

春季军犬的饲养管理

春季万物复苏,是仔幼犬生长发育、种犬繁衍后代的最佳时期,也是细菌繁殖、寄生虫复苏的季节。据资料统计,犬的传染病、流行性疾病60﹪都发生在冬春交替的季节,因此加强春季军犬的饲养管理尤其必要,下面就结合我部几年来的工作体会,与大家共同探讨。