1株奶牛源松鼠葡萄球菌的分离鉴定及耐药性分析

为确定奶牛垫料中是否含有病原菌并深入了解垫料中优势生长菌的类型、耐药性及致病性等情况,本试验进行了细菌分离培养、革兰染色镜检、生化试验鉴定、16S rDNA序列分析及同源性比对、药敏试验、小鼠致病性试验。结果显示,分离菌在普通营养琼脂平板上形成圆形、表面光滑的白色菌落,血琼脂平板上形成黏稠、较大的白色菌落。革兰染色镜检结果显示,分离菌为革兰阳性,球型,呈葡萄串状排列,或单个散落。生化试验结果显示,分离菌对木糖、葡萄糖、麦芽糖、果糖、乳糖、硝酸盐还原等呈阳性反应,而蜜二糖、木糖醇、山梨醇、尿素、V-P反应等呈阴性反应。16S rDNA序列分析结果显示,扩增的16S rDNA序列长度为1 298 bp,与松鼠葡萄球菌的核苷酸同源性达99.85%~100%;系统进化树结果显示,分离菌与松鼠葡萄球菌处于同一分支。动物试验结果表明,分离菌株对试验小鼠有较强的致病性,以0.2 mL/只（7.9×10⁸ CFU/mL）菌液的剂量接种小鼠,在48 h内死亡率为60%（3/5）。药敏特性分析结果显示,分离菌株对复方新诺明、氨苄西林等7种药物敏感,对克林霉素、头孢噻肟和头孢呋辛中度敏感,对青霉素、红霉素和林可霉素耐药。本研究为奶牛源松鼠葡萄球菌的分离鉴定及防控提供了参考依据。     

Research Advanced on Functions of Nutritional Regulation and Intestinal Barrier of Arginine

Arginine is one of the most important amino acids for animals,and participates in the synthesis and metabolism of many nutrients,plays a role in the development and function of the gastrointestinal tract.Intestinal barrier function is one of the most functions for intestine.The article will summarize the effect of arginine on intestinal barrier.It was explicated the influence on inteseinal immunity of Toll-like receptor.This research provided some references for further study of arginine regulation on animal production.     

广豆根种子生产标准操作规程(SOP)

根据《中药材质量管理规范》(GAP)的指导原则,对药用植物广豆根种子生产的环境条件、种植方法、田间管理、采收加工、贮藏、包装及运输进行了研究和规定,制定了药用植物广豆根种子生产标准操作规程(SOP),这对于保证药用植物广豆根优良品种的纯度和优良种性,提供足够人工栽培种源,促进中药材规范化生产具有重要意义。     

Temporal-spatial Filtering in the Preprocessing of Clinical EEG Signals

EEG spot-recording for clinical patients in the bed is always advocated due to easing patients and making them comfortable. But EEG signals recorded in the ward exposed to various noisesand interference is less effective in the EEG analysis. Atemporal-spatial filter is designed for noise removing of clinical EEG signals, the first filtering is temporal filtering using band-pass filter, and the second filtering is spatial filtering with ICA-based spatial filter. Experimental results indicate that various noises and interferences, such as power interference, blinking, eyes movements, muscle moments, ECG artifacts, etc., are removed effectively, individually or simultaneously.     

Orientation of Aim Client in Real Estate Development Based on EXCEL

In order to subdivide the market during the process of project planning and orient the group of client, then determine the sale price; on the condition that the traditional orientation procedure cant grasp the payment capability of the aim client, the anthors can adapt the powerful finance analysis ability of EXEL to design the procedure of determining the purchasing power and payments ability of the aim client, and shape a set of orientation system based on quantitative analysis method. The result is, the character of the aim client can be grasp accurately depend on this orientation system, and providing a scientific gist for the accurate orientation of price and lowing the risk of investment etc.     

双流道叶轮设计方法研究

双流道叶轮设计采用变宽流道设计法，容易造成高效区向大流量偏移设计点效率偏低，因此需要对流道面积进行控制。对现有文献中双流道叶轮设计方法进行了补充，较为详尽地介绍了流道面积、流道中线分点以及平面流道中线的确定方法。为双流道叶轮设计提供了参考。     

现代玻璃温室智能气动天窗系统设计与研究

为满足现代温室对室内环境越来越高的要求，采用快速、安全、可靠、低成本的气动自动化技术设计气动天窗系统，可以实现天窗系统开闭与开度的自动化调控。为此，讨论了气动天窗系统的原理，得出了驱动气缸负载模型以及各级风速下气缸负载特性，探讨了该系统可行性和特点。与传统技术比较，气动系统完全可以取代机械式开天窗，而且结构简单，操作方便，位置控制精度高，天窗开度和通风面积显著增加，增强了利用自然通风调节温室环境的能力。     

温室地下贮热系统设计研究

为了降低温室环境调控能耗。根据育苗不同阶段对气温与土壤温度的要求，设计了温室地下贮热系统，并阐述了系统的组成和贮热原理；同时，分析了各设计参数对系统贮热性能的影响。这样不仅为系统设计与理论分析奠定了基础，对其它类似地下贮热系统的设计、研究也具有参考意义。     

平地漫灌灌溉水净化与循环利用研究

为提高设施农业的水分利用率，设计了平地漫灌灌溉水净化与循环利用系统；阐述了系统的组成、节水灌溉原理与工作过程。理论分析可知，系统具有较高的输水利用率、灌水利用率和降水利用率，是农业节水灌溉的有效新途径。