紫花苜蓿GDP解离抑制蛋白基因cDNA的克隆、分析及烟草转化

采用RT-PCR方法,克隆得到紫花苜蓿(Medicago sativa L.)GDP解离抑制蛋白基因cDNA核心区域序列,命名为MsGDI1。序列分析结果表明,该cDNA核心区域全长1575 bp,包含一个1332 bp的最大开放阅读框,编码444个氨基酸。半定量RT-PCR分析结果表明,在盐胁迫和铝胁迫条件下,MsGDI1基因的表达随着胁迫时间的增加而上升,该基因可能与紫花苜蓿抗盐耐铝机理有关。借助于新构建的该基因超表达载体,用农杆菌介导方法转化烟草(Nicotiana tabacum L.)。转基因烟草植株PCR检测的结果表明,MsGDI1基因已经成功插入到烟草基因组中,为进一步研究该基因的功能奠定了基础。     

Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus Isolated in Shanxi Provine

In order to investigate the variation in S gene of porcine epidemic diarrhea virus (PEDV), the 4 strains of PEDV S gene nucleotide sequences were obtained, through RT-PCR amplification of tissue samples from Shanxi province. The obtained sequences and the deduced amino acid were analyzed and compared with the other published PEDV strains. Sequence analysis showed that compared with CV777 vaccine, there were 12 nucleotides insertions between 170 to 171 bp, 3 nucleotides insertions between 401 to 402 and 454 to 455 bp, 6 nucleotides deletion between 461 to 468 bp. The nucleotide and amino acid homologies were 99.2% to 99.8% and 98.6% to 99.7% respectively among 4 strains of PEDV S gene; Comparing with the strains isolated from China in 2011 to 2015, CV777 vaccine, attenuated DR13 and CV777, the nucleotide homologies were 95.0% to 98.5%,93.2% to 93.6%,92.1% to 92.9%,93.7% to 94.4%,respectively.The amino acid homology were 96.2% to 98.9%,91.9% to 92.9%,91.9% to 92.6%,92.9% to 94.0%, respectively. Phylogenetic analysis revealed that 4 strains of PEDV S gene belonged to the first group and had high correlative genetic relationship with the PEDV strains which isolated after 2010 in China, and had far correlative genetic relationship with the PEDV strains which isolated before 2010 in China, 2 strains of Japanese, 7 strains of South Korea, 2 vaccine strains. The results suggested that the prevalence of PEDV in Shanxi province had a more obvious variation. Therefore, it was necessary to develop a new vaccine to control the outbreak of PEDV.     

宁南山区土地生态系统服务价值变动对经济发展的影响——以隆德县为例

以宁夏回族自治区隆德县为例,通过最小二乘法的回归分析,对1996-2011年宁南山区土地生态系统服务价值变动对经济发展的影响进行了研究。研究结果表明:(1)水域生态系统服务价值变动对隆德县经济发展的影响最大,应加强对水域生态系统的保护;(2)林地生态系统服务价值变动对隆德县经济发展的影响最小,应在稳定林地建设的基础上,着重发展后续生态产业;(3)农田生态系统服务价值变动对隆德县经济发展为正相关影响,其他地类为负相关影响,应设法增加农田的生态系统服务价值,同时适当控制林地、草地、水域、难利用地的面积;(4)难利用地生态系统服务价值变动对经济发展的影响偏小,说明隆德县的经济发展较为依赖自然资源,现代化程度低,应进一步优化产业结构,提高经济发展水平。     

灌水模式对不同品种冬小麦群体生长特性和产量及蛋白质含量的影响

为筛选推荐节水高产品种提供依据,研究了不同灌水模式对冬小麦群体动态和产量及蛋白质含量的影响.以泰山23、泰山223、山农6号和石家庄8号为试验材料,设置3种灌水方式,印W1底墒水(生育期不浇水)、W2底墒水+春1水(拔节水-孕穗期)和W3底墒水+拔节水+开花水.主要研究了不同灌水方式下4个品种的产量构成因素、叶面积指数、干物质积累及籽粒蛋白质含量性状.结果表明,W1和W2、W3之间产量差异显著,W2和W3之间产量差异不显著,品种之间产量差异显著.叶面积和干物质积累量随着灌水次数的增加而增加,但不同品种之间表现不同,泰山23较其它3个品种变化较小.不同灌溉模式对试验品种均表现为随着灌水次数的增加蛋白质含量减少,W1显著高于W2和W3.根据本研究结果可知,说明泰山23具有较好产量潜力和品种适应性.     

春季解冻期棕壤坡面磷素迁移过程研究

春季解冻期土壤季节性冻融发生最为强烈,极易发生土壤侵蚀,也是磷素流失的关键时期。采用人工模拟降雨的方法,探究春季解冻期不同磷素背景值坡面产流产沙及磷素流失动态过程。结果表明:产流后14 min内径流量和泥沙量均呈现较好的线性分布,径流相关系数为0.969,泥沙相关系数为0.936;14~18 min内径流量缓慢增加,从18 min开始一直到产流结束径流量基本保持在3 100 ml/min,而泥沙则总体呈现先增大再减小的趋势;土壤背景值（APb）越高,径流、泥沙中磷浓度越高;径流中磷素流失比率均值APb₂₀坡面最大,且随着背景值的增大呈减小趋势;而泥沙中磷素流失比率均值变化则与径流不同,APb₄₀的坡面其流失比率最小,其他坡面差异较小;径流中磷素流失量与泥沙中磷素流失量呈线性关系,y=6.751x-0.628（R²=0.958）。     

番茄种质资源黄化曲叶病毒病抗性的鉴定与评价

鉴于目前商业番茄品种黄化曲叶病毒病抗性难以甄别,对86份番茄种质的番茄黄化曲叶病毒(TYLCV)抗性基因进行PCR检测并进行田间抗病性鉴定。结果表明,目前抗TYLCV番茄种质抗性基因主要是Ty-1/ty-1和Ty-1/ty-1+Ty-3a/ty-3,2种类型抗病种质(19份、23份)分别占总抗病种质(61份)的31.15%和37.70%。在仅含有Ty-1基因的25个番茄种质中,有80.00%田间表现抗病,而同时含有Ty-1和Ty-3a 2个基因的26个番茄种质中,田间抗病率达100%,说明抗性基因具有累加效应。通过抗性基因PCR方式进行抗病检测,准确率达到81.40%。同时推荐了M-17号、达丽等25份抗TYLCV番茄优良品种。     

An analysis of formation conditions for DBD transported charges of multineedle-to-cylinder

Dielectric barrier discharge (DBD) can produce homogenous discharge in normal temperature and atomsphere with low energy consumption, so it has broad development prospects in industrial applications. An experimental study is carried out to optimize the needles arrangement and supply parameters aiming at the multineedle-to-cylinder reactor with orthogonal design in combination with Lissajous figures. As it is konwn, the process of discharge is also the process of charge transfer; hence, the more charges are transported, the higher reaction efficiency in plasma is. The orthogonal design is established with transported charges as the goal factor, and supply voltage, supply frequency, needles length(NL), interneedle rotation angles(INRA) as factors. Variance analysis results show that the largest influence factor is supply voltage, followed by supply frequency; the electrode configuration of NL=(3.5+2) mm and INRA=45° matched with f=21 kHz makes transported charges per cycle biggest. The orthogonal experiment results are verified by further experiments, which is significant to improve the DBD plasma reaction efficiency.     

氮肥运筹对玉米干物质及氮素积累转运和产量的影响

2015～2016年进行田间氮肥试验,研究分次施肥和施氮量对玉米干物质、氮素积累特性和产量的影响.供试品种为郑单958,采用二因素裂区设置,主区2种施氮方式,全部基施和50％基施+50％大喇叭口期追施;副区68、113、181、249kg/hm24种施氮量,对照为不施氮肥,测定干物质和氮素积累量以及产量与产量构成.结果...     

爬山虎在阳泉市垂直绿化中的应用

垂直绿化能最大限度地利用有限的空间,提高绿化覆盖率,在最小范围内实现植物的多样性,并改善空气质量,减少热岛效应。在垂直绿化植物中,爬山虎由于其适应性强、成本低、见效快、宜推广,被广泛大量应用。该文就爬山虎在阳泉市垂直绿化中的应用误区及下一步的发展方向进行探讨,以指导爬山虎的合理使用。     

掺沙对盐渍化土壤水盐分布及其蒸发特性的影响

为了探明盐渍土水盐分布和土壤蒸发特性对掺沙的响应特征,以山东省滨州市无棣县"渤海粮仓"科技示范区土壤为研究对象,分析了不同掺沙量(沙-土质量比为0,10％,30％,50％和70％,分别以处理CK,T1,T2,T3和T4表示)对盐渍土水盐运移特征和土壤蒸发的影响.结果表明,0～5000 min,处理T1和T2表层0～1 ...