Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5) gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P<0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P<0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P<0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.     

Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.     

Study on Fans Cooling of Dairy Cowshed with Free Stall in Northern Mountainous Region of Hebei in Summer

The present objective of the study was to investigate the effect of fans cooling on dairy cow in northern mountainous region of Hebei in summer.Two cowsheds with the same structure and feeding technics were used to investigate the effect of fans on indoor temperature and humidity, physiological parameters and milking performance of dairy cow.The results showed that the average ambient temperature in cowshed with fans was 1.5℃ lower than that of the control cowshed, and the average wind velocity was 0.49 m/s higher than that of the control cowshed, reaching to 0.66 m/s.Temperature-humidity index (THI) of cowshed with fans was significantly lower than that of the control shed during the period of fans running (P<0.05).Besides, the rectal temperature and respiration rate of dairy cows in cowshed with fans were significantly lower than of the control cowshed (P<0.05), and the milk yield per cow per day was 17.0% higher than that of the control cowshed (P<0.05), however, the milk fat, milk protein and milk sugar were no significant difference from the control (P>0.05).Overall, fans could decrease indoor environment, moderate heat stress and increase performance and health of dairy cows in northern mountainous region of Hebei.     

不同锌肥施用方式对番茄果实的影响

以番茄为试材,设置底施、随水冲施、叶面喷施锌肥为处理,以不施锌肥为对照,研究了锌肥不同施用方式对番茄生长指标和生理指标的影响。结果表明:叶面喷施锌肥处理番茄果实直径平均值比不施锌肥的CK高6.15mm;同时可以提高番茄果实内IAA、ZR、DHZR、GA3含量,降低ABA含量;增加果实中SOD、POD活性,提高植株的抗逆性,延缓衰老。同时改善番茄果实品质,叶面喷施处理与对照相比,果实糖酸比提高9.5%,维生素C含量提高了近1倍,可溶性固形物含量提高23.4%。施用锌肥处理各项指标优于不施锌肥的处理,其中以叶面喷施效果最佳。     

马铃薯晚疫病的发生、流行规律及其对产量的影响

为探明马铃薯晚疫病发病规律及其对产量的影响,以清水为对照,开展了枯草芽孢杆菌BS-10和银法利(氟菌·霜霉威)防治马铃薯晚疫病效果试验。试验结果表明,马铃薯晚疫病发生和流行速度快,从出现中心病株到全部植株枯死仅17~24 d,病情呈逐渐加重趋势,对产量影响极大,如不防治或防治不当,可造成减产2/3;试验所用药剂均不能完全抑制晚疫病的发生,相对而言,银法利防效优于枯草芽孢杆菌BS-10。     

华南三省(自治区)部分西番莲种植区花叶病病原检测及EAPV多聚蛋白基因序列分析

<正>西番莲(Passiflora edulis Sims.)是热带亚热带地区重要经济作物,我国福建、广东、广西、海南等地广泛种植[1]。调查发现,我国南方多地西番莲花叶病为害严重,症状表现为叶片花叶、黄化、斑驳、畸形,甚至整株褪绿,严重影响西番莲的产量和品质。已报道能引起西番莲花叶症状的病毒有：黄瓜花叶病毒属(Cucumovirus)的黄瓜花叶病毒(cucumber mosaic virus, CMV),     

我国松毛虫发生率的空间分布格局及其驱动因子

了解我国松毛虫发生的空间分布格局及其驱动因子, 对松毛虫暴发的预防?控制和治理具有重要意义?本研究基于我国各行政区2004年-2012年间每年松毛虫发生面积及其寄主植物面积, 使用一般线性模型分析气候因子是否以及如何驱动我国松毛虫发生的空间分布格局?结果表明:在全国尺度上, 最暖季降水量是松毛虫发生的主要气候驱动因子, 松毛虫平均发生率与最暖季降水量显著正相关?在最暖季降水量的作用下, 我国松毛虫的发生呈现明显的空间分布格局, 最暖季降水量较高的南方和北方部分地区松毛虫发生率较高, 而降水量较低的西北和青藏地区松毛虫发生率较低?我们的研究结果可为松毛虫暴发的预防和管理工作提供指导?     

不同基因型大麦苗期对干旱胁迫的响应及HvPIPs基因表达特征分析

为研究不同基因型大麦苗期对干旱胁迫的响应,挖掘优质抗旱的大麦种质资源,以4份不同基因型大麦(2个高抗品种ZDM5430和ZDM5458,2个干旱敏感型品种7DCADA和IL-12)为材料,利用聚乙二醇(PEG-6000)模拟干旱条件,综合分析不同品种大麦的苗期抗旱性差异;并通过对不同品种根系进行PIPs实时定量分析,研究水通道蛋白AQPs基因与干旱胁迫之间的应答关系。结果表明,持续干旱胁迫会使大麦生长变缓,各处理苗长、根长、叶含水量和根含水量都呈下降趋势,其中,4个品种的大麦叶含水量在干旱胁迫14 d时下降最为显著,与对照相比,其降幅分别为29.14%、52.64%、21.67%和72.15%。干旱胁迫对大麦苗期干物质积累量和根冠比的影响较小,各处理间差异不显著;但持续干旱后,抗旱品种ZDM5430的根冠比呈上升趋势,在胁迫7、14、21 d时较CK分别增加了4.92%、42.86%和21.05%。随着胁迫时间的延长,大麦旗叶叶绿素含量和根系活力也呈下降趋势,其中ZDM5430和ZDM5458较对照组降幅较小,说明抗旱品种的形态指标、叶绿素含量及根系活力受干旱胁迫的影响较小。通过PIP...     

氮磷钾配施对红花产量及其有效成分在器官中分配的影响

于2018—2019年在河南科技学院试验田,以百农红花1号为材料,设计氮、磷、钾三因素和无肥(N0、P0、K0)、低肥(N1、P1、K1)、中肥(N2、P2、K2)、高肥(N3、P3、K3)四水平试验,以T1(N0P0K0)为对照,T2 (N0P2K2)、T3(N1P2K2)、T4(N2P2K2)、T5(N3P2K2)、T6(N2P0K2)、T7(N2P1K2)、T8(N2P3K2)、T9(N2P2K0)、T10(N2P2K1)、T11(N2P2K3)为不同配施水平,共11个处理,研究氮、磷、钾肥用量及配比对红花产量的影响,探讨氮、磷、钾不同用量及配比下红花生育期各器官中有效成分分配规律。结果表明：红花花产量随氮肥用量的增加而增加,以T5最高,与T1相比增加59.44%,随磷肥和钾肥用量的增加呈先上升后下降趋势,均以T4最大,与T1相比增加42.87%;红花种子产量随氮、磷、钾肥用量的增加呈先上升后下降的趋势,均以T4最高,与T1相比增加116.52%。氮、磷、钾肥不同用量及配比对红花生育期各器官中黄酮含量影响有相同规律,从苗期到成熟期,叶和茎黄酮含量均表现为先下降后上升的趋势,根中黄...     

不同杀菌剂对当归主要病害的防效及经济效益比较

针对甘肃当归病害逐年加重态势, 采用田间随机区组试验, 分析比较70%噁霉灵WP?50%氯溴异氰尿酸WP和80%代森锰锌WP对当归主要病害防效及经济效益, 以期筛选出最佳防控药剂?结果表明:3种杀菌剂均能不同程度防治当归病害, 其中以50%氯溴异氰尿酸WP 1.86 g/L对褐斑病?麻口病防效最佳, 分别为70.38%~78.75%?84.48%~91.04%, 70%噁霉灵WP 0.34 g/L和80%代森锰锌WP 7.92 g/L次之, 对褐斑病防效分别为59.14%~63.72%?58.14%~62.84%, 对麻口病防效分别为78.61%~83.57%?78.97%~83.57%?50%氯溴异氰尿酸WP 1.86 g/L能很好地抑制当归抽薹, 抑制率达58.63%~63.15%?3种杀菌剂均能明显提高当归药材产量构成因子, 以80%代森锰锌WP 7.92 g/L处理增产增收效果最好, 两年平均增产量高达5 559.91 kg/hm2, 平均经济效益为194 136.65元/hm2, 但投入产出比最低, 为1∶480.63?50%氯溴异氰尿酸WP 1.86 g/L对当归病害防效及抽薹抑制率最高, 投入产出比为1∶634.02, 在此基础上综合考虑病害防效和经济效益, 50%氯溴异氰尿酸WP 1.86 g/L可作为生产上防控当归主要病害的主推药剂?