一株鸭新城疫强毒的分离鉴定和分子特性分析

从山东地区发病鸭中分离到一株新城疫病毒(SDLP09),经测定,其鸡胚半数致死量(ELD50)、鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)、6周龄鸡静脉接种致病指数(IVPI)分别为107.84/0.1 mL、48.5 h、1.80、2.36,表明该新城疫病毒为强毒.通过分析其融合蛋白(F)发现,F蛋白多肽裂解位点为112RRQKRF117,符合NDV强毒株裂解位点氨基酸序列.F基因分型及氨基酸同源性比较发现,SDLP09株属于基因Ⅶ型,与野鸭源强毒NDV同源性更高,提示着该毒株可能来源于野鸭.     

蔬菜高效施药装置药量专家系统测试

为了满足蔬菜高效施药的精确性和便捷性,借助信息技术手段将专家知识库存贮在单片机芯片中,通过快捷的选择调用达到蔬菜高效施药的目的。本文介绍了高效施药装置专家系统的工作原理,并通过试验验证了其理论施药量和实际控制值具有较好的一致性。     

银耳α–微管蛋白基因的克隆及序列分析

 根据GenBank上已发表的其他真菌α–微管蛋白基因序列中的保守区域设计简并引物,扩增出银耳α–微管蛋白基因的cDNA部分序列。分别利用SEFA-PCR方法和RACE技术,克隆了银耳α–微管蛋白基因的DNA和cDNA全长序列。银耳α–微管蛋白基因DNA全长1 962 bp,含有9个内含子,cDNA全长1 347 bp,编码448个氨基酸。生物信息学分析结果表明：银耳α–微管蛋白基因的cDNA序列与新型隐球酵母有80%相似性,编码的蛋白质属于Tubulin_FtsZ超家族中的α–微管蛋白亚家族,氨基酸序列中存在保守的GTP结合区域GGGTGSG,系统发育树显示银耳与新型隐球酵母距离最近。     

甘南牦牛繁育技术

通过对甘南牦牛品种选育、选配、杂交改良、配种、饲养管理等关键技术的规范,提高甘南牦牛繁殖率与供种能力,为甘南牦牛的科学繁育提供技术支撑,推动甘南牦牛产业高质量发展。     

In Vitro Fertilization (IVF) in Straws and a Short Gamete Coincubation Time Improves the Efficiency of Porcine IVF

The present study was designed to evaluate three different in vitro fertilization (IVF) systems: a straw‐IVF system with 10 min of coincubation, a straw‐IVF system with 6‐h coincubation and the microdrop‐IVF system with 6‐h coincubation (the traditional IVF system used routinely in most of IVF laboratories) in an attempt to reduce polyspermic penetration ( Experiment 1 ). When the straw‐IVF system was tested in combination with two coincubation times, the use of 10 min of coincubation significantly increased (p < 0.001) the penetration rate and the efficiency of fertilization (67.7 ± 6.4% vs 31.9 ± 6.5% and 41.5 ± 2.5% vs 17.6 ± 2.5% for 10 min and 6 h, respectively), while there were no significant differences in the incidence of monospermy between both systems (64.3 ± 5.1% and 67.7 ± 3.4%, for 10 min and 6 h, respectively). The penetration rate in the 6‐h microdrop‐IVF system was higher (93.8 ± 3.6%; p < 0.001) compared with the 10‐min straw‐IVF system (67.7 ± 6.4%), however, monospermy was severely reduced (25.0 ± 4.3% vs 67.7 ± 3.4%, for the 6‐h microdrop‐IVF system and 10‐min straw‐IVF system, respectively). The efficiency of the IVF showed similar values between microdrop and 6‐h straw‐IVF systems, but efficiency was significantly improved (p < 0.05) when the 10‐min straw‐IVF system was used. Experiment 2 was designed to compare porcine in vitro embryo production in two IVF systems, the 6‐h microdrop‐IVF system (1000 sperm per oocyte) and 10‐min straw‐IVF system (30 000 sperm per oocyte). The blastocyst formation rates tended (p = 0.06) to be higher when the 10‐min straw‐IVF system was used compared with the 6‐h microdrop‐IVF system. In addition, the number of total cells per blastocyst increased significantly (p < 0.05) in the 10‐min straw‐IVF system. These results showed that the 10‐min straw‐IVF system is an effective way to decrease polyspermic penetration, and improve the efficiency of fertilization and the quality of blastocysts in terms of cell number per embryo.     

春兰菌根的显微结构观察

应用石蜡切片法,借助于光学显微镜观察春兰无菌组培苗、接菌组培苗的根及野生状态下的新生营养根、老根,结果表明:接菌组培苗的根及野生状态下的新生营养根、老根,其皮层组织细胞内有大量的菌丝结等兰科菌根的典型结构,而无菌组培苗的根则无菌丝、菌丝结等结构。菌根真菌自外皮层薄壁通道细胞侵入根的皮层细胞,春兰菌根是典型的地生兰菌根。     

一株白僵菌的分离鉴定及其对微小牛蜱的致病力试验

为了得到用于防控微小牛蜱的高毒力菌株,本试验从野外微小牛蜱饱血雌蜱体表分离出一株白僵菌,通过形态学和分子生物学方法进行了鉴定,确认该分离株为球孢白僵菌.致病力研究表明,当孢子浓度达到108孢子/mL,该菌株对微小牛蜱饱血成蜱的致死率即可达到100%,为微小牛蜱的生物防控试剂的研究及应用奠定了基础.     

干旱胁迫条件下不同抗旱性苹果砧木内源激素含量的变化

以抗旱性差异显著的两种苹果砧木楸子(Malus prunifolia)和平邑甜茶(Malus hupehensis Reld)为材料,研究了干旱胁迫对两种砧木根和叶中脱落酸(ABA)、生长素(IAA)、赤霉素(GAs)和玉米素核苷(ZR)含量的影响.结果表明:干旱胁迫下,与非抗旱砧木平邑甜茶相比,抗旱砧木楸子中抑制生长...     

北京地区规模化奶牛场牛病毒性腹泻病血清学调查

对北京郊区6个未进行牛病毒性腹泻病（BVD）免疫牛场的546份奶牛血清样品,使用牛病毒性腹泻抗体ELISA试剂盒进行检测,并对其中3个牛群进行牛病毒性腹泻病毒（BVDV）血清抗原筛查。共检出阳性血清514份,总阳性率94.1%,其中5个牛场的场内血清抗体阳性率在95%以上。牛病毒性腹泻持续性感染牛（PI牛）筛查的3个牛群均有阳性牛检出。结果表明,北京地区规模化奶牛场存在牛病毒性腹泻感染和接触史,应采取净化措施进行控制。