Effects of selection on food intake in the adult mouse

SUMMARY: Replicated lines of mice were selected High and Low for adjusted food intake and contemporaneous control lines were maintained. The selection criterion was food intake between 8 and 10 weeks, adjusted by phenotypic regression on mean body weight at 8 and 10 weeks of age to reduce correlated changes in body weight. Responses are given for the first 23 generations of selection, after which adjusted food intake had diverged by a factor of 1.7-1.95. A small correlated response in body weight occurred and mice from the High line were slightly heavier: at 10 weeks of age body weight had diverged by a factor of 1.09-1.11. The realized within-family heritability varied between the replicates from 0.16-0.27 from which a mean estimated mass selection heritability (h(2) = 0.35±0.05) was derived. Mice from the Low line were fatter, however not significantly, because of a High between replicate variance (p > 0.05). Differences in growth over the selection period may account for around 5% of the divergence and increased maintenance costs associated with the larger lean mass of the high lines may explain a further 5%. Mice from the High lines spilled significantly (p < 0.05) more food which accounted for 23% of the divergence in apparent food intake. The heat increment of feeding, brown adipose tissue activity and locomotor activity all appear to be unchanged. ZUSAMMENFASSUNG: Auswirkungen der Selektion auf Futteraufnahme in der adulten Maus In einem Experiment mit Wiederholungen wurden M?uselinien auf hohe und niedrige korrigierte Futteraufnahme selektiert und korrespondierende Konttrollen gehalten. Das Selektionskriterium war die Futteraufnahme im Alter von 8 bis 10 Wochen, die mittels ph?notypischer Regression korrigiert wurde, um die K?rpermasse m?glichst konstant zu halten. Der direkte Selektionserfolg über die ersten 23 Generationen ist beschrieben. Die Linien divergierten zu diesem Zeitpunkt bezüglich des Selektionsmerkmals um 70 bis 95%. In der K?rpermasse trat ein geringfügiger korrelierter Selektionserfolg auf. Die Tiere der 'high'-Linie waren im Alter von 8 bis Wochen ca. um 6 bis 11% schwerer. Die realisierte Intra-Familien-Heritabilit?t variierte zwischen den Wiederholungen zwischen 0.16 und 0.27, woraus sich eine mittlerer Heritabilit?tskoeffizient von h(2) = 0.35±0.05 für die Massenselektion ergab. M?use der 'low'-Linie hatten mit 10 Wochen 2.4% (P > 0.05) und mit 17 Wochen ca. 7% (P < 0.05%) mehr Fett. Mit Unterschieden im Wachstum lassen sich weniger als 5% der Linienunterschiede in der Futteraufnahme erkl?ren. Der h?here Erhaltungsbedarf, der aus einer h?heren fett-freien K?rpermasse in der 'high'-Linie resultiert, k?nnte weitere 5% erkl?ren. Tiere der 'high'-Linie verstreuten deutlich (P < 0.05) mehr Futter, worauf sich 23% der Divergenz in der scheinbaren Futteraufnahme zurückführen lie?en. Die Aktiv?t des braunen Fettgewebes, die lokomotorische Aktivit?t und die fütterungsbedingte Wàrmeproduktion sind scheinbar unver?ndert.     

Chicken anaemia virus—a glimpse of the future?

This paper uses background information about chicken anaemia virus as a guide to how the study and control of virus diseases of poultry may develop in the future. It is predicted that ‘new’ viruses will be discovered in poultry, many of which will be difficult to grow in vitro and whose pathogenicity may appear uncertain. When new diseases/syndromes arise in the future, it should be a priority activity to define their pathology. The limitations of currently available virus diagnostic methods are highlighted. The possibility of vaccinating against economically important subclinical disease is discussed, as is the use of recently developed technologies in differentiating virus strains and in developing new vaccines.     

The role of lectures in veterinary education

OBJECTIVE: To study the role of lectures from the perspective of staff and students involved in the veterinary course at The University of Queensland. METHODS: The Nominal Group Technique of Delbecq et al, which provides the maximum opportunity for group members to put forward points, was used to help develop a questionnaire which was completed by 351 students (a response rate of 84%) and 35 staff (76%) from the five years of the veterinary course, and was analysed using the SAS System for Windows. RESULTS: Almost all the staff and students agreed that lectures should fulfil many roles including stimulating and motivating students and encouraging them to think, as well as presenting ideas and concepts and an indication of the structure and relevance of the material. They should provide a guide for effective deep learning, but not encourage rote (or superficial) learning. A smaller percentage of staff and even fewer students agreed that lectures did fulfil these roles, especially those directed at encouraging students to look beyond simple memorisation of facts. The perceived disparity between reality and the ideal was greater amongst the older, clinical students than amongst their more junior colleagues. CONCLUSIONS: The focus of attention in lectures needs to change from the superficial, rote learning of information to deep, active learning directed at using information to solve problems that are perceived by the students to be relevant. If done in a stimulating and interesting way, this should develop skills in reasoning and critical analysis as well as providing a framework for storage and recall. It should also increase the motivation towards learning both during the veterinary course, and over the professional lifetime. Furthermore, the place of the lecture in veterinary education needs to be reassessed regularly in the face of newly-emerging educational technology.     

Use of skin staples for rapid closure of gastrointestinal incisions in the treatment of canine linear foreign bodies

A technique was developed for closure of gastrotomy and enterotomy incisions using disposable skin staples. The technique was used successfully in three dogs with gastrointestinal linear foreign bodies that required a gastrotomy and one or more enterotomies. The method allows for secure closure of gastrointestinal incisions and minimizes the surgical time in patients requiring multiple gastrointestinal incisions.     

Therapeutics of companion exotic marsupials.

Exotic marsupials are becoming popular companion pets, yet little pharmacologic information is available to the practitioner. Species include sugar gliders (Petarus breviceps), South American (Brazilian) short-tailed opossums (Monodelphis domestica), brushtail possum or "phalanger" (Trichosurus vulpecula), and wallabies: Bennett's (Macropus rufogriseus rufogriseus) and Tammar or Dama (Macropus eugenii). Guidelines for logical choices of medications based on allometric scaling, gastrointestinal tract anatomy and physiology, and liver metabolism are given. An example of scaling and a formulary based on anecdotal, published medications are provided.     

Metabolic and mitogenic activities of insulin-like growth factor-1 in interleukin-1-conditioned equine cartilage

OBJECTIVE: To determine response of interleukin-1alpha (IL-1alpha)-conditioned equine articular cartilage explants to insulin-like growth factor-1 (IGF-1). Sample Population-Cartilage from the trochlea and condyles of the femur of a clinically normal 4-year-old horse. PROCEDURE: Effects of IGF-1 (0 to 500 ng/ml) after addition of IL-1alpha were evaluated by assessing matrix responses, using a sulfated glycosaminoglycan (GAG) assay, matrix 35SO4 GAG incorporation, and release of GAG. Mitogenic response was assessed by 3H-thymidine incorporation into DNA and fluorometric assay of total DNA concentration. RESULTS: Human recombinant IL-1alpha (40 ng/ml) increased the amount of labeled GAG released and decreased labeled and total GAG remaining in explants, and IL-1alpha decreased mitogenic response. Addition of IGF-1 counteracted effects seen with IL-1alpha alone. In general, IGF-1 decreased total and labeled GAG released into the medium, compared with IL-1alpha-treated explants (positive-control sample). Values for these variables did not differ significantly from those for negative-control explants. A significant increase in total and newly synthesized GAG in the explants at termination of the experiment was observed with 500 ng of IGF-1/ml. Labeled GAG remaining in explants was greater with treatment at 50 ng of IGF-1/ml, compared with treatment with IL-1alpha alone. Concentrations of 200 ng of IGF-1/ml abolished actions of IL-1alpha and restored DNA synthesis to values similar to those of negative-control explants. CONCLUSIONS AND CLINICAL RELEVANCE: IGF-1 at 500 ng/ml was best at overcoming detrimental effects associated with IL-1alpha in in vitro explants. These beneficial effects may be useful in horses with osteoarthritis.     

Epitope-tagged insulin-like growth factor-I expression in muscle

Development of a recombinant insulin like growth factor I (IGF-I) that is distinguishable from its endogenous counterpart would provide a powerful tool for delineating the role of IGF in myogenesis. Therefore, the objective of this study was to create an epitope-tagged IGF-I that retains biological activity and determine whether expression of this construct is possible in muscle tissue following direct DNA injection. Expression vectors were created that encoded porcine IGF-I containing a T7 (11-amino acid) epitope-tag (TIGF). Immunoreactivity of the purified recombinant TIGF was confirmed using monoclonal antibodies. Biological activity was evaluated by examining differentiation of myoblasts cultured with TIGF or transfected with TIGF plasmid DNA. Addition of purified TIGF to myoblast cultures stimulated (P < 0.05) muscle creatine kinase levels similar to insulin (10(-5) M). Likewise, transfection of L6A1 with TIGF DNA hastened (P < 0.01) differentiation compared to control pcDNA-transfected myoblasts. The integrity of the recombinant protein was confirmed using a sandwich-configured enzyme linked immunosorbent assay. Finally, recombinant TIGF DNA was injected in porcine muscle and the ability to detect TIGF protein was evaluated. TIGF expression was detected in muscle fibers of injected porcine muscle. These data show that a T7 amino acid tag placed on the amino terminus of the IGF-I protein remains intact during processing and does not interfere with the biological activity of the molecule. Use of this DNA construct is an excellent tool for investigating the role of IGFs in control muscle development and provides a model to investigate other regulators of animal growth.     

Evaluation of intratesticular injection of glycerol for nonsurgical sterilization of dogs

OBJECTIVE: To determine efficacy of intratesticular injection of glycerol as a means to sterilize dogs. ANIMALS: Six 2- to 4-year-old sexually intact male dogs. PROCEDURE: Testicular volume, serum testosterone concentration, and number of sperm in ejaculate were determined before and at weekly intervals for 12 weeks following intratesticular injection of a 70% glycerol solution (1 ml/testis). Testicular tissue specimens for histologic evaluation were obtained 4, 8, and 12 weeks following injection. RESULTS: Number of sperm in ejaculate following glycerol injection was not significantly different from number before injection. Compared with preinjection values, serum testosterone concentrations were significantly less 5, 9, and 10 weeks following injection, although libido and ejaculate characteristics were unchanged. Testicular swelling was evident 24 to 48 hours following injection, and testicular volume decreased starting on week 5 following treatment. Seminiferous tubules and evidence of normal spermatogenesis were detected in testicular biopsy specimens obtained from all dogs 12 weeks following injection. CONCLUSION AND CLINICAL RELEVANCE: Intratesticular injection of a 70% glycerol solution did not result in azoospermia and sterility in dogs.     

Dose dependency of prednisolone on the establishment of Echinococcus multilocularis infection in an alternative definitive host, Mongolian gerbil

This study revealed the dose dependency of prednisolone tertiary-butylacetate (PTBA) treatment on the establishment of Echinococcus multilocularis in the small intestine of Mongolian gerbil (Meriones unguiculatus) and that some of the physiological parameters of host were correlated with the doses of PTBA and establishment of the worm. Twenty Mongolian gerbils were divided into 5 groups, according to the doses of PTBA; 0 mg, 0.5 mg, 2 mg, 5 mg and 10 mg per head. All animals were injected intraperitoneally with PTBA every other day from 6 days before to 6 days after infection. Doses of PTBA and the number of worms recovered at 7 days post-infection showed a positive correlation (r = 0.929, P < 0.0001). The increase of total protein (TP) and the decrease of the percentage of lymphocytes in the peripheral leukocytes were dependent on doses of PTBA (TP: r = 0.811, P < 0.0001, percentage of lymphocyte: r = -0.92, P < 0.0001). The TP and the percentage of lymphocyte also correlated with the number of worms recovered (TP: r = 0.617, P = 0.0049; percentage of lymphocyte: r = -0.800, P < 0.0001).