点对点和集总帧通信下网络化CNC系统的位置同步控制

为了提高网络化数控系统协同运动的同步控制性能,提出了一种将点对点通信模式和集总帧通信模式结合应用在环形网络的周期通信规划,以使主节点能及时获取所有轴的反馈信息。提出了相应的位置同步算法和运动消息预估算法,并对根据算法设计的位置同步控制器进行了稳定性证明。实验表明,该通信规划相应的位置同步控制器具有良好的同步性能。     

The rehabilitation of an irrigation system along the Yellow River

The People's Victory Irrigation System which diverts water from the Yellow River of China covers a total irrigable area of 59 000 ha. The system encountered some serious problems in the first decade of its operation — salinity and waterlogging of irrigated land, siltation of irrigation and drainage channels, as well as a low efficiency of water use. This paper describes a series of structural and functional measures which have been adopted in the past 24 years for the rehabilitation of the system, including: improvement of the existing drainage system and construction of new drainage system on agricultural land, conjunctive use of surface and groundwater supplies, a comprehensive programme to reduce the levels of siltation as well as the implementation of improved water management practices. A model for the optimal operation of the system by using system analysis theory as an aid in reducing the operation and maintenance (O & M) costs has been developed in recent years. The results are that the soil salinity has been controlled and the agricultural production has increased whilst the efficiency of water use has improved and the siltation levels reduced. Experience gained on this system has been successfully used in developing and managing other irrigation projects along the lower reaches of the Yellow River. The proposed procedure of solving the said optimal model has also embodied some benefits from reducing O & M costs in operation.     

Improvement of Appearance Traits of elite Rice Zhenshan 97B by Molecular Introgression of Targeted about 800 kb Interval from Indica Rice Jiafuzhan Genome

Rice appearance trait is mainly mean grain length (GL), grain width (GW), ratio of length and width (L/W), chalkiness (CH) and transparence (TR). Appearance is the first impression, and rice appearance traits effect significantly the value of commodity. Hybrid rice is relatively low quality, especially on rice appearance quality,which restricts partially its extend and badly export of hybrid seed and rice in China.Shanyou 63, the most famous hybrid rice combination of China, could not be also sustainable application because of its poor qualities. In this studies, We attempt to improve appearance quality of the elite rice maintainer of Zhenshan 97B by using molecular approach of genomic interval targeted introgression. Three F2 populations were constructed to study the classical and molecular genetic of rice appearance traits, at the same time, molecular marker-asisted toimprove the main maintainer line, Zhenshan 97B were performed. There some following results:Three F2 populations were constructed based on crosses of Guangluai 4×Jiafuzhan (GJ popu.), Minghui 86×Jiafuzhan (MJ popu.) and Zhenshan 97B×Jiafuzhan (ZJ popu.). ZJ popu and GJ popu were produced to reconstruct the linkage maps related with rice appearance traits based on BSA, and detect QTL of target traits based on IM.In ZJ popu, 9 QTLs of target traits were detected. The interval, RM169～RM516 on Chromosome 5, was genetic locus of GW, L/W, CH and BW, and the explained variances were 10.9%, 14.9%, 12.0% and 14.2%, respectively.RM214 on chromosome 7, RM339 on chromosome 8 were genetic loci if CH and BW, and the explained variances were 9.4%, 10.0%, 11.0% and 12.1%. besides there is a genetic locus, RM347, of G/W on chromosome 3, and the explained varance was 10.3%. In GJ popu, 5 QTLs of rice appearance traits were detected. The interval, RM169-RM516 on Chromosome 5, were also the genetic loci of GW, CH and BW, and the explained variances were 9.0%, 12.5%, and 13.6%, respectively. RM264～RM80 on chromosome 8 was the genetic loci of GW amd L/W,and the explained variances were 14.7% and 13.4%. The genotypes of all QTLs, except RM214, from Jiafuzhan contributed to improving riee appearance traits.The research was to improve the rice appearance quality of Zhenshan 97B by introgressing QTLs of rice appearance traits from Jiafuzhan, a donor parent, by means of MAS in the process of recurrent backcrossing, 4 SSR markers were used to detect the target genotypes from donor parent, and 70 SSR primers to identify the genetic background of recurrent parent. Consequently, there were 19 target individuals in BC3, in which the mean value of GL, GW, L/W, CH and BW were 6.06±0.04mm, 2.50±0.02mm, 2.43±0.02, 29.5±2.7 and 29.4±2.7%, respectively,and the mean homologous degree (HD) to recurrent parent is up to 98.22%. Compared to Zhenshan 97B, GL, CH and BW decreased 0.17 mm, 34.9% and 34.5% respectively and however L/W increased 0.19. which accounted separately for 25.37%, 54.79%, 54.57% and 10.50% of thedifference of every trait between two parents. It is very luckly to find a individual, 13BC2-3BC3 line, whose GL, GW, G/W, CH and BW were 6.04 mm, 2.43 mm, 2.49,2.5% and 2.5% separately, There is very important signification for it to be used in hybrid rice breeding improving appearance traits. In the research, MAS efficiency of 3 loci (with 4 markers ) was 5.68% and coincident degree was 73.08%.     

Cloning and Sequence Analysis of Lipoxygenase Gene cDNA from Cucumber Fruit （Cucumis sativus L.）

Lipoxygenases are nonheme-iron-containing dioxygenases that catalyze the hydroperoxidation of unsatrated fatty acids containing a cis, cis-1,4-pentadiene structure producing hydroperoxy acids with conjugated dienes. LOX activity has been found in a wide range of plants. Typical substrates for LOX in plants are linoleic acid and linilenic acid fatty acids. The function of various LOXs in plants is unknown, but their participation in all stages of plant growth and development has been suggested （Hildebrand, 1989; Siedow, 1991）. Some of the physiological processes in whicn lipoxygenses have been implicated include wounding （Saravitz and Siedow, 1996）, pathogen attack （Melan et al., 1993）, seed germination （Kato et al., 1992）, fruit ripening （Ferrie et al., 1994）, plant senescence （Paliyath and Droillard, 1992）. The study on the role of lipoxygenase in ripening and senescence fruit focused on tomato and strawberry. Cloning LOX gene of cucumber fruit will make us further understand the molecularaction mode of this enzyme during fxuit ripening and senescence. In this paper we isolated the partial nucleotide sequences of cucumber fiuit lipoxygenase gene and discuss the characterization of it.     

A Simple and Reliable Assay for Detecting Specific Nucleotide Sequences in Plants Using Optical Thin-film Biosensor Chips

Here we report the adaptation and optimization of an efficient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-film biosensor chips to detect unique transgenes in genetically modified （GM） crops and SN-P markers in model plant genomes. Briefly, aldehyde-attached sequence-specific singlestranded oligonucleotide probes are arrayed and covalently attached to a hydrazine-derivatized biosensor chip surface. Unique DNA sequences （or genes） are detected by hybridizing biotinylated PCR amplicons of the DNA sequences to probes on the chip surface. In the SN-P assay, target sequences （PCR amplicons） are hybridized in the presence of a mixture of biotinylated detector probes and a thermostable DNA ligase. Only perfect matches between the probe and target sequences, but not those with even a single nucleotide mismatch, can be covalently fixed on the chip surface. In both cases, the presence of specific target sequences is siL, nified by a color change on the chip surface （gold to blue/purple） after brief incubation with an anti-biotin IgG horseradish peroxidase （HRP） to generate a precipitable product from an HRP substrate.     

双充种室大蒜单粒取种装置设计与试验

针对当前大蒜机械化播种单粒合格率低、漏充率高的问题,该研究设计了一种双充种室大蒜单粒取种装置。通过分析与计算,确定了取种装置关键部件参数,阐述了双充种室结构可降低蒜种漏充的作业机理。采用EDEM软件进行了单因素仿真试验,分析了一级取种勺速度、种层厚度对充种性能及种群规律的影响,得到第二充种室内充入蒜种与被清掉蒜种的速度变化关系,证明了装置设计合理性。运用Box-Behnken中心组合试验方法,以一级取种勺速度、取种速比、种层厚度作为试验因素,以单粒合格率和漏充率作为评价指标,开展了三因素三水平正交试验。利用Design-Export8.0.6数据分析软件,建立各试验因素与评价指标的数学回归模型,并对试验因素进行了参数优化。圆整后一级取种勺速度为0.12 m/s、取种速比为0.75的条件下,种层厚度范围为360～390 mm开展室内验证试验,单粒合格率、漏充率、重播率均值分别为95.38%、1.18%、3.44%,变异系数分别为0.32%、6.11%和4.15%,验证试验结果与模型预测值相对误差小于5%。品种适应性试验试验结果表明取种装置对3类供试大蒜Ⅱ级蒜种适应性较优,蒜种机械损伤率为0.52%,符合标准要求。田间试验结果与优化结果一致,作业效果优于现有爪式循环、勺链式、轮勺式大蒜单粒取种装置,该研究可为解决大蒜播种机取种漏充问题提供指导参考。     

甘蓝SSR标记在近缘种青花菜的通用性及其应用

本研究对50条甘蓝SSR引物在其近缘种青花菜中的通用性进行了分析,结果表明,38对引物在青花菜上可有效扩增,扩增产物分子量在100～1500bp,有效扩增比率为76%,其中18%具有较好的多态性,揭示了两种作物基因组间存在一定的相似性。同时利用获得的多态性较好的9对引物对青花菜进行基因型鉴定和遗传多样性分析,20份青花菜基因型中共检测到51个基因位点,平均每个引物组合可扩增出5.67个条带,多态性为66.7%。多引物组合可鉴别出所有青花菜基因型。聚类分析结果表明同一来源的基因型间具有相近的遗传基础,且聚类与熟性具有一定的相关性。本研究为今后青花菜种质资源的收集、利用及分子标记辅助育种提供了新的SSR标记和参考。     

Seasonal Changes of Mineral Nutrients in Fruit and Leaves of ‘Newhall’ and ‘Skagg's Bonanza’ Navel Oranges

In southern Jiangxi province of China, ‘Newhall’(Citrus sinensis Osbeck) navel orange presented a conspicuous symptom of boron (B) deficiency in mature leaves, whereas B deficiency symptoms were not manifested on ‘Skagg's Bonanza’(C. sinensis Osbeck) navel orange. In this study, changes in concentrations of B, calcium (Ca), potassium (K), magnesium (Mg), manganese (Mn), and zinc (Zn) were comparatively investigated in the structural parts of the fruit (rind and pulp) and leaves (old leaves from last season and spring-flush leaves from current year) of ‘Newhall’ and ‘Skagg's Bonanza’ navel orange during the growing season. Two peaks of B concentrations were observed in the rind of the two cultivars during fruitlet growth and fruit enlargement, respectively. Boron concentrations were relatively high in the rind during fruitlet growth, and then decreased in both rind and pulp, whereas, during middle and late fruit enlargement significant increases were found for B in both rind and pulp of the two cultivars. Boron concentrations in old leaves of ‘Newhall’ decreased progressively and remained relatively low, whereas that of ‘Skagg's Bonanza’ was relatively high and changed slightly as the season progresses. Both Ca and K concentrations were above the critical threshold values, while their dynamics were reverse to that of B in fruit and leaves during certain times. Old leaf Mg concentrations of samples at 140 days after full bloom from the two cultivars and spring-flush leaves from ‘Newhall’ were below the threshold limit for sufficiency. In addition, Mg in old leaves was much lower from ‘Newhall’ than from ‘Skagg's Bonanza’. Spring-flush leaf concentrations of Mn and Zn and Mn concentrations in old leaves from ‘Newhall’ were relatively lacking during middle and late season, which accelerated the occurrence of B deficiency symptoms on mature leaves of ‘Newhall.’     

Impacts of soil erosion on organic carbon and nutrient dynamics in an alpine grassland soil

The impact of soil erosion on the nutrient dynamics in alpine grassland soils is still an essential problem. Selecting a grass-covered hillslope in eastern Tibet Plateau, the cesium-137 (¹³⁷Cs) technique was used to determine the impacts of soil erosion on soil organic carbon (SOC), total nitrogen (TN), total phosphorus (TP), and total potassium (TK). The ¹³⁷Cs data revealed that there were distinct soil redistribution patterns in different hillslope positions because of the influences of slope runoff, plant coverage and grazing activity. For the upper slope, soil erosion first decreased downward, followed by soil deposition in its lower part. In contrast, for middle and toe slopes, there was an increasing soil erosion along a downslope transect. Across the lower slope, soil erosion showed an irregular variation. Influenced by the selective transport of water erosion, SOC, TN and TP storage decreased with increasing soil erosion in upper, middle and toe slopes. In contrast, SOC, TN and TP storage varied little with soil erosion in the lower slope. On the whole hillslope, TK storage also varied little with soil erosion due to the large amount of potassium elements derived from soil parent materials. Particularly noteworthy was the greatest storage of SOC, TN and TP in the lower slope where most obvious net soil erosion occurred, which is closely related to the humus accumulation combined with gravel separation as well as weathering and pedogenesis of parent rocks induced by soil freeze-thaw.